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Vol.2 , No. 6, Publication Date: Oct. 29, 2015, Page: 95-100
 column followed by evaporative light scattering detection to quantify the glucosylceramide content. The method was validated for selectivity, precision, and accuracy. The recovery of glucosylceramides from spiked matrix samples was approximately 100%, and the limits of detection and quantification were 0.03 mg/mL and 0.1 mg/mL, respectively. The electron light scattering detector response fits well into a power equation for calibration. The assay precision (relative standard deviation values) was less than 3% for repeatability and less than 4% for reproducibility.&picture=http://www.aascit.org/aascit/decorators/img/journal/907.jpg)
| [1] | Xun Yan, Analytical Sciences, Amway Corporation, Ada, MI, USA. |
| [2] | Douglas J. Vredeveld, Analytical Sciences, Amway Corporation, Ada, MI, USA. |
| [3] | Stephen R. Missler, Analytical Sciences, Amway Corporation, Ada, MI, USA. |
In this report, we describe a simple and rapid HPLC method to analyze glucosylceramide content in wheat extracts and product formulations containing wheat extracts. The HPLC separation of glucosylceramides from other glycolipids was performed on a normal-phase (diol) column followed by evaporative light scattering detection to quantify the glucosylceramide content. The method was validated for selectivity, precision, and accuracy. The recovery of glucosylceramides from spiked matrix samples was approximately 100%, and the limits of detection and quantification were 0.03 mg/mL and 0.1 mg/mL, respectively. The electron light scattering detector response fits well into a power equation for calibration. The assay precision (relative standard deviation values) was less than 3% for repeatability and less than 4% for reproducibility.
Keywords
Evaporative Light Scattering Detector (ELSD), Glucosylceramides, Normal-Phase Chromatography, Wheat Extracts
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