Vol.1 , No. 1, Publication Date: Aug. 29, 2014, Page: 9-20
[1] | Ogunleye, Akin O., Department of Microbiology, Lead City University, Ibadan, Nigeria, P.O. Box 23101 U.I. Postoffice, Ibadan, Nigeria. |
[2] | Ayansola, Oyeronke T., Department of Microbiology, Lead City University, Ibadan, Nigeria. |
This study was carried out on the storage rot of yam tubers Dioscorea spp in Ibadan, Nigeria. The Dioscorea spp examined were Dioscorea rotundata (white yam) and Dioscorea alata (water yam) which were the common species available at the time of study. The study area was visited six times in three months for collection of rotted yams which were selected randomly at a particular barn. The symptoms of the diseases were described and their associated organisms isolated and identified. The diseases include dry rot and soft rot which accounted for 54.22% and 45.78% of post-harvest diseases of the yams respectively. Out of 60 yam samples examined 52 yam samples had fungi isolates.The fungi isolated include Aspergillus niger, Lasiodiplodia theobromae, Rhizopus stolonifer, Fusarium oxysporum, Aspergillus flavus, Aspergillus ochraceus, Aspergillus lilacinum, Penicillium sp, Pythium sp., Sclerotium rolfsii, Allomyces arbuscula and Rhodotorula sp. The fungal isolates had the following percentage frequencies of occurrences respectively: 19.29%, 12.14%, 14.29%, 12.85%, 13.57%, 2.14%, 2.86%, 6.43%, 3.57%, 7.86%, 3.57%, and 1.43%. Pathogenicity test carried out confirmed the following organisms as the pathological agents of the yam rot: Lasiodiplodia theobromae, Aspergillus niger, Sclerotum rolfsii, Rhizopus stolonifer, Alloymyces arbuscular, Fusarium oxysporum, Penicillium sp. and Aspergillus flavus. Aflatoxin levels of all Aspergillus isolates were determined. The potential hazards posed by the aflatoxins are discussed.
Keywords
Aflatoxin, Mycoflora, Rot, Pathogenicity, Dioscorea Spp., Fungi
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