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American Journal of Microbiology and Biotechnology

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Identification and Characterization of Two Lipolytic and Thermophilic Bacterial Strains Isolated from Saudi Arabia Environment

American Journal of Microbiology and Biotechnology
Vol.5 , No. 3, Publication Date: Nov. 5, 2018, Page: 50-60

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Authors

[1]	Mohammed Abdullah Al-Omair, Department of Chemistry, Faculty of Science, King Faisal University (KFU), Hofuf, Saudi Arabia.

Abstract

Lipases are one of the most valuable classes of enzymes of high economic importance. Bacterial lipases vary widely in enzymatic properties and substrate specificities. Consequently, they are currently receiving much attention because of their potential applications in various industrial processes and biotechnological applications as in fat, food ingredients, detergents, surfactants, textile industries and oil processing. Microbial lipases have wide application in the processing of leather, domestic, industrial wastes and pharmaceutical industries. The need for thermostable lipase enzymes is steadily rising and the isolation of lipases from thermostable microorganisms is highly requisite. In a screening program for isolation of thermophilic lipase-producing bacteria, a number of thermophilic bacteria were isolated from Al- Hassa region, Saudi Arabia. Among 93 isolates, potent bacterial candidates were identified based on biochemical characteristics, RAPD-PCR, and 16S rRNA gene sequencing. Phylogenetic analysis revealed their closeness to the thermophilic Burkholderia pseudomalllei (B. pseudomalllei) and Staphylococcus pasteuri (S. pasteuri) with optimal growth at 50°C for both strains and pH 8.0 and 7.5, respectively. An inducible nature of lipolytic enzyme synthesis using oils was demonstrated. Salt stress studies revealed that S. pasteuri and B. pseudomalllei have the ability to tolerate NaCl salt up to 2% and 2.5%, respectively. Both S. pasteuri and B. pseudomalllei are the highest thermophilic bacteria generating lipase.

Keywords

Thermophilic, Lipase, Rapid PCR, 16S rRNA, Burkholderia pseudomalllei, Staphylococcus pasteuri

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