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Vol.5 , No. 2, Publication Date: Aug. 2, 2018, Page: 29-36
. The fungus spores were treated with nitrous acid (HNO<sub>2</sub>) and N-methyl-N’nitro-N-nitroso guanidine (NTG) for strain improvement. The mutated strains were evaluated for lipase production via fermentation of POME. Fungal growth, free fatty acid percentage, lipase activity and pH were analyzed in the course of fermentation using standard procedures. The result of the mutants and their lipase activity showed that Nitrous acid mutant (NA50) had the highest fungal growth and lipase activity to be 16.07 ± 0.03 x 10<sup>6</sup> cfu/mL and 11.69 ± 0.07 U/mL respectively. The lipase yield of the mutant NA50 was 39.00% improvement than the wild strain. Statistically, comparing lipase activity of NA50 strain to others showed a significant difference (p< 0.05). Subsequently, NTG mutants showed that strain NN120 had a 22.65% improvement in lipase activity over Nitrous acid mutant (NA50) strain and a 70.63% improvement over the wild strain <i>A. niger</i>. The results indicated that POME can be utilized for lipase production and the process can be enhanced through strain improvement using nitrous acid and N-methyl-N’nitro-N-nitroso guanidine&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Cyprian Erumiseli Oshoma, Department of Microbiology, University of Benin, Benin City, Nigeria. |
| [2] | Elvis Eseosa Osawaru, Biochemistry Division, Nigerian Institute for Oil Palm Research, Benin City, Nigeria. |
| [3] | Osayi Brenda Isichei-Ukah, Department of Microbiology, University of Benin, Benin City, Nigeria. |
| [4] | Henry Uzo Oshilonyah, Department of Medical Microbiology, Central Hospital Laboratory, Agbor, Nigeria. |
Lipases of microbial origin represent the most widely used class of enzymes in biotechnology. This study was aimed at production of lipase by using chemically altered strains of Aspergillus niger isolated from Palm Oil Mill Effluent (POME). The fungus spores were treated with nitrous acid (HNO2) and N-methyl-N’nitro-N-nitroso guanidine (NTG) for strain improvement. The mutated strains were evaluated for lipase production via fermentation of POME. Fungal growth, free fatty acid percentage, lipase activity and pH were analyzed in the course of fermentation using standard procedures. The result of the mutants and their lipase activity showed that Nitrous acid mutant (NA50) had the highest fungal growth and lipase activity to be 16.07 ± 0.03 x 106 cfu/mL and 11.69 ± 0.07 U/mL respectively. The lipase yield of the mutant NA50 was 39.00% improvement than the wild strain. Statistically, comparing lipase activity of NA50 strain to others showed a significant difference (p< 0.05). Subsequently, NTG mutants showed that strain NN120 had a 22.65% improvement in lipase activity over Nitrous acid mutant (NA50) strain and a 70.63% improvement over the wild strain A. niger. The results indicated that POME can be utilized for lipase production and the process can be enhanced through strain improvement using nitrous acid and N-methyl-N’nitro-N-nitroso guanidine
Keywords
Lipases, Palm Oil Mill Effluent (POME), Mutagenic Chemicals, Strain, Fermentation
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