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ISSN: 2375-3005

American Journal of Microbiology and Biotechnology

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Molecular Detection of Heavy Metals Tolerant and Hydrocarbonoclastic Enzymes in Some Bacteria from Refinery Effluent

American Journal of Microbiology and Biotechnology
Vol.5 , No. 2, Publication Date: May 16, 2018, Page: 18-24

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Authors

[1]	Dangmwan Samuel Denwe, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
[2]	Enimie Endurance Oaikhena, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
[3]	Muhammad Muktar Namadi, Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria.
[4]	Ali Ahmed Haroun, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
[5]	Omolola Elizabeth Oladapo, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
[6]	Alhaji Isyaku Abdullahi, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
[7]	Sherifat Bukola Ayuba-Buhari, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.

Abstract

Petroleum refinery transforms crude oil into more refined end products such as gasoline, kerosene, aviation fuel, diesel, and lubricating oils with effluent release as by product. The study was intended at isolation, characterization and identification of some bacteria isolates from refinery drains with molecular capabilities for removal of heavy metals and with hydrocarbonoclastic enzymes for reclamation of some petroleum liquid waste polluted sites. Five bacteria were isolated each, for their potentials to remove heavy metals and exert hydrocabonoclastic effect on refinery hydrocarbons. Phenol chloroform method was employed in extracting DNA from the bacteria and gene bands for heavy metals and hydrocarbon depletion were separated by agarose gel electrophoresis sequel to PCR amplification. The selected bacteria from the effluent for heavy metals removal were S. aureus, E. coli, P. aeruginosa, Proteus vulgaris and K. puemoniae which were screen for Ncc A, Chr B and Czc A gene bands while those that were screened for hydrocarbonoclastic gene bands such as Alk B and C2,3O were Serretiamarcescens, Streptomyces sp., P. aeruginosa, Proteus vulgaris and K. puemoniae. Three of the bacteria that had both potentials for heavy metals tolerance and hydrocarbon degradation like P. aeruginosa, P. vulgaris and K. puemoniae were thought to be the most ideal for further biodegradative studies under different environmental conditions.

Keywords

Hydrocarbonoclastic, Refinery, Bacteria, Degradation, Heavy Metal

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