ISSN: 2375-3005
American Journal of Microbiology and Biotechnology  
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The Influence of Different Challenge Doses of Highly Pathogenic Avian Influenza on the Efficacy of Different Avian Influenza Vaccine
American Journal of Microbiology and Biotechnology
Vol.3 , No. 3, Publication Date: May 10, 2016, Page: 18-22
2303 Views Since May 10, 2016, 600 Downloads Since May 10, 2016

Nourhanne M. Said, The Central Laboratory for Evaluation of Veterinary Biologics, Cairo, Egypt.


Lamiaa M. Omar, The Central Laboratory for Evaluation of Veterinary Biologics, Cairo, Egypt.


Ahmed A. El-Sanousi, The Virology Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.


This study aim to investigate the influence of different challenge doses of HPAIV on the efficacy of different AI vaccine was studied by use of two different types of inactivated AI vaccines H5N1, and H5N2 and their potency were evaluated by using different doses of HPAI challenge virus. Groups of specific pathogen free chicken (SPF) were vaccinated with the recommended dose of the two vaccines according to the manufacturer, and another group was kept as control. Four weeks post vaccination (DPV) both vaccinated and control chicken groups were bled for the detection of Ab titer in response to vaccination using HI (haemagglutination inhibition test). The vaccinated chicken with the two vaccines were subdivided into 4 subgroups to be challenged by the doses (104, 105, 106, 107) EID50 of HPAI challenge virus correspondingly. The results revealed that the Ab titer produced with H5N1 vaccine was 8 log-2 and 7 log2 for H5N2 vaccine. The protection percent of the vaccinated chickens was calculated for each challenge dose for each vaccine. The protection percent of the chicken vaccinated with H5N1 vaccine was 100% in case of all AIv challenge doses while it was 90% for the chicken groups vaccinated with H5N2 in cases of (104, 105, 106) challenge doses and 85% in case of (107) challenge doses. These results demonstrate that chicken vaccinated with good quality inactivated AI vaccines under good condition were protected from clinical signs (morbidity) and deaths (mortality) caused by AIv infection even with high doses.


HPAIV, Challenge Dose, Immune Response, Haemagglutination Test, H5N1 and H5N2 Vaccines, Efficacy and Potency


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