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Efficacy of Aqueous and Ethanolic Leaf Extracts of Ocimum gratissimum L. and Eucalyptus camaldulensis Dehnh. on AEDES Larvae

AASCIT Journal of Biology
Vol.4 , No. 3, Publication Date: Dec. 21, 2018, Page: 47-52

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Authors

[1]	Jacqueline Azumi Badaki, Department of Biological Sciences, Federal University, Lokoja, Nigeria.
[2]	Emmanuel Obu Ngwoke, Department of Biological Sciences, Federal University, Lokoja, Nigeria.
[3]	idemi Benjamin Atteh, Department of Biological Sciences, Federal University, Lokoja, Nigeria.

Abstract

AedesAedes mosquitoes transmit diseases such as Yellow fever that had resulted in millions of human death. Outbreaks of Yellow fever has been encountered in a few states in Nigeria since December 2017. Synthetic commercial larvicides used in the control of the vector mosquitoes are non-biodegradable, resulting in negative environmental consequences. This has led to a shift to exploration of plant phyto-chemicals as better alternatives because of the advantage of being eco-friendly. The efficacy of aqueous and ethanolic extracts of Ocimum gratissimum and Euclyptus camaldulensis on Aedes larvae was investigated. The qualitative phytochemical screening of the plant extracts showed more presence of phytochemicals such as tannin, saponin, flavonoids, glycosides and alkaloids in the ethanolic extracts than aqueous extracts. The ethanolic extracts of the plant recorded higher percentage of larvae mortality than the aqueous extracts. Ethanolic plant extracts also recorded very low LC₅₀ and LC₉₀ values unlike the aqueous extracts, indicating high larvae toxicity. The findings indicated that ethanolic plant extracts had very high lethal effect on Aedes larvae compared to their aqueous extracts. Although, the ethanolic extract of O. gratissimum alone was more potent than E. camaldulensis and their combined formulation [O.gratissimum: LC₅₀ = 731ppm; E. camaldulensis: LC₅₀=773ppm; O. G+E. C: LC₅₀ = 766ppm]. This infers that the performance of the ethanolic plant extracts did not improve when they were combined. The mortality of Aedes larvae was dose and time-dependent with increase in larvae mortality being directly proportional to time period of exposure of the larvae to the plant extracts. Further studies will be required to isolate the active ingredients responsible for the larvicidal effect in O. gratissimum.

Keywords

Eucalyptus Camaldulensis, Ocimum Gratissimum, Lethal Concentration, Aedes Larvae

Reference

[01]	Mohomed, A. A., Tarek, I. A. A. M., Zarrag, I. A. A. (2014). Larvicidal and repellent effect of some Tribulus terrestris L., (Zygophyllaceae) extracts against the dengue fever mosquito, Aedes aegypti (Diptera: Culicidae). Journal of the Saudi Society of Agricultural Sciences, 20:13-16.
[02]	World Health Organization (2014). A Global Brief on Vector-Borne Diseases. http://apps.who.int/iris/bitstream/10665/111008/1/WHO_DCO_WHD_2014.1_eng.pdf.
[03]	Service, M. W. (1974). Survey of relative prevalence of potential Yellow fever vectors in North-West Nigeria. Bulletin World Health Organization, 50:487-494.
[04]	Adeleke M. A. (2008). Mosquito species breeding in artificial containers in Ikenne farm settlement, Ogun State, Nigeria. BSc. Dissertation submitted to the University of Agriculture, Abeokuta, 35p.
[05]	Aigbodion FI. and Uyi OO. (2013). Temporal distribution of and habitat diversification by some mosquitoes (Diptera: Culicidae) Species in Benin City. Nigeria. Journal of Entomology, 10: 13-23.
[06]	Chukwuekezie, O. C., Nwangwu, U. C., Ogudu E. O., Okoronkwo, A. I., Okoye, C. K., Onuora, E. N., Obisi, N. V., Anielozie, C., Orizu F. U. and Igu, P. O. (2016). A cross sectional survey of Yellow fever and Dengue virus vectors in four communities of Ayamelum Local Government Area (L. G. A), Anambra State, Southeast Nigeria. New York Science Journal, 9 (3):1- 6.
[07]	Braks, M. A. H., Honorio, N. A., Lourenco-de-Oliveira, R., Juliano, S. A. (2003). Convergent Habitat Segregation of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Southeastern Brazil and Florida. Journal of Medical Entomology, 40 (6): 785-794.
[08]	Maciel-de-Freitas, R., Codeço, C. T., Lourenço-de-Oliveira, R. (2007). Daily survival rates and dispersal of Aedes aegypti females in Rio de Janeiro, Brazil. American Journal of Tropical Medicine and Hygiene, 76: 659-665.
[09]	Knio, K. M., Usta, J., Dagher, S., Zournajian, H., Kreydiyyeh, S. (2008). Larvicidal activity of essential oils extracted from commonly used herbs in Lebanon against the seaside mosquito. Ochlerotatus caspius. Bioresource Technology, 99:763–768.
[10]	World Health Organization (2009). WHO Recommended Insecticides for Indoor Residual Spraying Against Malaria. WHO Publication.
[11]	Jirakanjanakit, N. P., Rongnoparut, S., Saengtharatip, T., Chareonviriyaphap, S., Duchon C. B. and Yoksan, S. (2007). Insecticide susceptible/resistance status in Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus (Diptera: Culicidae) in Thailand during 2003 - 2005. Journal of Economic Entomology, 100 (2): 545-550.
[12]	Sarwar, M., Ahmad, N. and Toufiq, M. (2009). Host plant resistance relationships in chick pea (Cicer arietinum L.) against gram pod borer (Helicoverpa armigera Hubner). Pakistan Journal of Botany, 3047-3052.
[13]	Zhu, J., Zeng, X., O’neal, M., Schultz, G., Tucker, B., Coats, J., Bartholomay, L. and Xue, R. D. (2008). Mosquito larvicidal activity of botanical-based mosquito repellents. Journal of the American Mosquito Control Association, 24 (1): 161-168.
[14]	Rahuman, A. A. and Venketesan, P. (2008). Larvicidal efficacy of five cucurbitaceous plant leaf extracts against mosquito species. Parasitology Research, 103: 133-139.
[15]	Kamaraj, C., Bagavan, A., Rahuman, A. A., Zahir, A. A., Elango, G., Pandiyan, G. (2009). Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitology Research, 104: 1163-1171.
[16]	Anupam, G., Nandita, C. and Goutam, C. (2012). Plant extracts as potential mosquito larvicides. Indian Journal of Medical Research, 135: 581-598.
[17]	Orwa, C., Mutua, A., Kindt, R., Jamnadass, R., Anthony, S. (2009). Agroforestree Database: a tree reference and selection guide version 4.0. World Agroforestry Centre Publication, Kenya, 4p.
[18]	Badaki, J. A. Awulu E. A., Alade O. M. (2018) Preliminary Study on Bioactivity of Anopheles Mosquito Exposed to Ocimum gratissimum Formulated Candles. American Association of Science and Technology Journal of Biology, 4 (2):21-24.
[19]	Sedaghat, M. M., SaneiAli, R., Khnavi, M., Abai, M. R., Hadjiakhoondi, A., Mohtarami, F., Vatandoost, H. (2010). Phytochemistry and larvicidal activity of Eucalyptus camaldulensis against malaria vector, Anopheles stephensi. Asian Pacific Journal of Tropical Medicine, (2010):841-845.
[20]	World Health Organization (2005). Guidelines for Laboratory and Field Testing of Mosquito Larvicides. World Health Organization Pesticide Evaluation Scheme (WHOPES) Publication, 41p.
[21]	Harborne, J. B. (1973). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman and Hall, London, 279p.
[22]	Trease, G. E. and Evans, W. C. (1989). Pharmacognosy. 11^th (ed). ELBS/Bailliere Tindall Ltd, London, pp: 60-75.
[23]	Suwannee, P., Amara, N., Maleeya, K. and Usavadee, T. (2006). Evaluations of larvicidal activity of medicinal plant extracts to Aedes aegypti (Diptera: Culicidae) and other effects on a non-target fish. Insect Science, 13 (3):179-188.
[24]	Nzelibe, H. C., Chintem, D. G. W. (2015). Larvicidal Potential of Leaf Extracts and Purified Fraction Ocimum Gratissimum against Culex Quinquefasciatus Mosquito Larva. International Journal of Science and Research, 4 (2): 2255-2256.
[25]	Kalaivani, K., Senthilnathan, S. and Ganesan, A. (2012). Biological activity of selected Lamiaceae and Zingiberaceae plant essential oils against the dengue vector Aedes aegypti L. (Diptera: Culicidae). Parasitology Research, 3 (110): 1261-1268.
[26]	Sosan, M. B., Adewoyin, F. B. and Adewunmi, C. O. (2001). Larvicidal properties of three indigenous plant oils on the mosquito Aedes aegypti. Nigerian Journal of Natural Products Medicine, 5: 30–33.
[27]	Sujatha, C. H., Vasuki, V., Mariappan, T., Kalyanasundaram, M., Das, P. K. (1998). Evaluation of plant Extracts for biological activity against mosquitoes. International Pest Control, 30:122–124.
[28]	Ghosh, A., Chowdhury, N and Chandra, G. (2012). Plant extracts as potential mosquito larvicides. Indian Journal of Medical Research, 135: 581-598.
[29]	Sumroiphon, S., Yuwaree, C., Arunlertaree, C., Komalamisra, N. and Rongsriyam, Y. (2006). Bioactivity of citrus seed for mosquito-borne diseases larval control. Southeast Asian Journal of Tropical Medical Public Health, 37 (3): 123-7.
[30]	Okigbo, R. N., Ogbonnanya, O. U. (2006). Antifungal effects of two tropical plants extracts Ocimum gratissimum and Afromaomum melegueta on post-harvest yam Discorea spp rot. African Journal of Biotechnology, 5 (9): 727-731.
[31]	Amadioha, A. C, Obi, V. I. (1999). Control of anthracnose disease of cowpea by Cymbopogon citratus and Ocimum gratissimum. Journal of Applied Entomology and phytopathology, 34 (1-2): 85-89.
[32]	Okigbo, R. N., Ajale, A. N. (2005). Inhibition of some human pathogens with the tropical plant extracts Chromolineena odorata and Citrus aurantifolia and some antibiotics. Interational Journal of Molecular Medicine, 1:34-40.
[33]	Usunobun, U. and Uwadiae, E. (2016). In vitro Medicinal Studies on Ocimum gratissimum Leaves. ARC Journal of Pharmaceutical Sciences, 2 (2): 1-5.
[34]	Sani, I., Abdulhamid, A., Bello, F. (2014). Eucalyptus camaldulensis: Phytochemical composition of ethanolic and aqueous extracts of the leaves, stem-bark, root, fruits and seeds. Journal of Scientific and Innovative Research, 3 (5): 523-526.
[35]	Milugo, T. K., Omosa, L. K., Ochanda, J. O., Owuor, B. O., Wamunyokoli, F. A., Oyugi, J. O. and Ochieng, J. W. (2013). Antagonistic effect of alkaloids and saponins on bioactivity in the quinine tree (Rauvolfia caffra sond.): further evidence to support biotechnology in traditional medicinal plants. BMC Complementary and Alternative Medicine, 13:285.