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Vol.5 , No. 4, Publication Date: Dec. 26, 2018, Page: 59-64
. In vitro shoots of sour jujube were selected as materials. Expanding young leaves were selected as explants. The effect of basal medium, thidiazuron concentration and culture protocol on shoot regeneration was examined. Results showed that two-step culture was better than one-step culture. First, leaf explants were cultured on the basal medium supplemented with thidiazuron (TDZ) and indole-3- acetic acid (IAA) for callus induction and adventitious buds initiation under dark culture, and then subcultured on the basal medium supplemented with IAA and gibberellic acid (GA3) for shoot buds elongation. The optimal media for shoot buds formation and elongation were WPM medium containing 0.5 - 1mg l-1 TDZ and 0.1 or 0.5 mg l-1 IAA and WPM medium containing 0.1 mg l-1 IAA and 0.5 mg l-1 GA3 separately. The effect of light on shoot regeneration was also examined. The adventitious buds regeneration was observed when leaf explants were cultured in the light, but in this case, the buds were smaller buds directly formed from leaf explants, and couldn’t further develop and elongate to form shoots. The two-step regeneration protocol may be applicable to the improvement of this crop by genetic engineering in the future.&picture=http://www.aascit.org/aascit/decorators/img/journal/923.jpg)
| [1] | Qingrong Sun, Shandong Institute of Pomology, Taian, China. |
| [2] | Qiuzhu Guan, Shandong Institute of Pomology, Taian, China. |
| [3] | Hongyan Sun, Shandong Institute of Pomology, Taian, China. |
A highly efficient two-step culture protocol was developed for adventitious shoot regeneration from in vitro leaf explants of sour jujube (Zizyphus spinosus Hu.). In vitro shoots of sour jujube were selected as materials. Expanding young leaves were selected as explants. The effect of basal medium, thidiazuron concentration and culture protocol on shoot regeneration was examined. Results showed that two-step culture was better than one-step culture. First, leaf explants were cultured on the basal medium supplemented with thidiazuron (TDZ) and indole-3- acetic acid (IAA) for callus induction and adventitious buds initiation under dark culture, and then subcultured on the basal medium supplemented with IAA and gibberellic acid (GA3) for shoot buds elongation. The optimal media for shoot buds formation and elongation were WPM medium containing 0.5 - 1mg l-1 TDZ and 0.1 or 0.5 mg l-1 IAA and WPM medium containing 0.1 mg l-1 IAA and 0.5 mg l-1 GA3 separately. The effect of light on shoot regeneration was also examined. The adventitious buds regeneration was observed when leaf explants were cultured in the light, but in this case, the buds were smaller buds directly formed from leaf explants, and couldn’t further develop and elongate to form shoots. The two-step regeneration protocol may be applicable to the improvement of this crop by genetic engineering in the future.
Keywords
Adventitious Buds, Bud Elongation; Direct Regeneration, Sour Jujube
Reference
| [01] | Amutha S, Muruganantham M, Ganapathi A (2006) Thidiazuron-induced high-frequency axillary and adventitious shoot regeneration in vigna radiata (L) Wilczek. In Vitro Cellular Developmental Biology - Plant 42:26-30. |
| [02] | Brand MH (1993) Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviceberry (Amelanchier arborea) shoots in vitro. Plant Cell, Tissue and Organ Culture 35: 203-209. |
| [03] | Dhaka N, Kothari SL (2002) Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in helianthus annuus L. Plant Cell Reports 21:29-34. |
| [04] | Gao Q, Wang B, Saimaiti T, Xu M, Luo S (2016). Agrobacreium-mediated CBE genetic transformation of Hami Zizyphus jujube. Northern Horticulturae, (18):94-98. |
| [05] | Gu XF, Zhang JR (2005) An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants. Plant Cell Reports 23:775–779. |
| [06] | Huang S, Xie S, Huang C, Zhang Z (2002) Studies on tranquillizing effect of jujuboside. Journal of Dalian university 23:90-92. |
| [07] | Kim MK, Sommer HE, Bongarten BC, Merkle SA (1997) High-frequency induction of adventitious shoots from hypocotyl segments of liquidambar styraciflua L. by thidiazuron. Plant Cell Reports 16:536-540. |
| [08] | Liu C, Zhang X, Ma H (1995) Studies of techniques of miniature propagation in wild jujube. Acta Bot Boreal-Occient Sin 15:301-306. |
| [09] | Liu X, Ren Y, Ren X (2004) Study on the relation of tissue structure and drought resistance on Zizyphus spinosus (Bunge) Hu. Leaf. J of Henan Vocation-Technical Teachers College 32:45-47. |
| [10] | Li A, Zhang G, Wang X, Li S, Shi D (2000) Preliminary report on biological characteristics and reproduction techniques of medical plant – Zizyphus jujuba var. spinosa Hu. Inner Mongolia Forestry Science & Technology 2:29-32. |
| [11] | Lloyd GB, McCown BH (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot culture. Combined Proceedings – International Plant Propagator's Society 30:421-427. |
| [12] | Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cellular Development Biology-Plant 29:92-96. |
| [13] | Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant 15:473-497. |
| [14] | Nie H, Ye J, Liu K, Zhang Y, Han D (2005) A study of sour jujube as rootstock for jujube. Northern Fruits 3:34-35. |
| [15] | Pan Q (2002) Advances of jujue witches’ broom research and control methods. Beijing Agricultural Science 3:4-8. |
| [16] | Peng Z, Zhang H, Cheng S, Guo W (1995) Protective effect of semen Ziziphi Spinosae on SOD reduction in mice with endotoxin fever. J of Chinese herb 20:369-370. |
| [17] | Rai VR (2002) Rapid clonal propagation of Nothapodytes foetida (Wight) Sleumer – A threatened medicinal tree. In Vitro Cellular Development Biology-Plant 38:347-351. |
| [18] | Reyes-Vera I, Potenza C, Barrow J (2008) Hyperhydricity reversal and clonal propagation of four-wing saltbush (Atriplex canescens, Chenopodiaceae) cultivated in vitro. Australian Journal of Botany 56: 358–362. |
| [19] | Rikiishi K, Takakazu M, Masahiko M, Kazuyoshi T (2008) Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos. Breeding Science 58:129-135. |
| [20] | Shao X, Wang Z, Zhang J, Feng S, Gao F, Zheng J (2005) Afforestation technology of jujube trees based on wild jujube in Taihang mountain area. J of Mountain Science 23:381-384. |
| [21] | Sun Q, Sun H, Zheng H, Shi Y (2000) Study on the plant regeneration from leaves of sour jujube (Zizyphus spinosus Hu.). Journal of Friut Science 17:48-51. |
| [22] | Sun Q, Liu Q, Sun H (2002) Effects of culture conditions on shoot regeneration from leaves of sour jujube (Zizyphus spinosus Hu.). Journal of Friut Science 19:24-26. |
| [23] | Sun Q, Sun H., Zhou G (2016). Study of adventitious bud regeneration from stem segments of jujube cultivar Luzao3. Shandong Agricultural Sciences 48 (2): 12-14. |
| [24] | Wang B, Peng D, Liu L, Sun Z, Zhang N, Gao S (2007a) An effficient adventitious shoot regeneration system for ramie (Boehmeria nivea Gaud) using thidiazuron. Botanical Studies 48:173-180. |
| [25] | Wang H, Wu Y, An F, Gu P, Zhang Z (2007b) The homology analysis for sequences of 16S rDNA and tuf gene of phytoplasma from jujube witches′ broom and wild jujube witches′ broom. Scientia Agricultura Sinica 40:2200-2205. |
| [26] | Xu M, Dai P, Luo Sh. Gao Q, Ayidingguli H (2014). Studies on adventitious bud regeneration system of Zizyphus jujuba Hami Leaves. Journal of Xinjiang Agricultural University 37 (3):185-190. |
| [27] | Zheng Y, Qian S, You Z (2006) Advances in study on pharmacological effects of semen Zizyphus spinosae. Sichuan Journal of Physiology Science 28:35-37. |
| [28] | Zhou J, Liu M, Hou B (1998) Advances in research on witch-broom disease of Chinese jujube. Journal of Friut Science 15:354-359. |
