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International Journal of Agricultural Sciences and Natural Resources

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A PCR-Based Analysis of Genetic Diversity of Rhizoctonia Oryzae-Sativae in Bangladesh

International Journal of Agricultural Sciences and Natural Resources
Vol.4 , No. 3, Publication Date: Aug. 8, 2017, Page: 15-21

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Authors

[1]	Mohammod Hossain, Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh.
[2]	Surapareddy Sreenivasaprasad, School of Life Sciences, University of Bedfordshire, Bedfordshire, UK.
[3]	Muthu Meena, Warwick Crop Centre, University of Warwick, Warwick, UK.
[4]	Md Abu Taher Mia, Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh.

Abstract

Experiments were conducted to know the genetic diversity of Rhizoctoniaoryzae-sativae. Aggregate sheath spot infected disease samples were collected from four locations viz. Bogra, Comilla, Gazipur and Rajshahi districts of Bangladesh. Sixty AFLP and nineteen SSR markers were screened in PCR- based analysis to generate polymorphisms in R. oryzae-sativae. Out of 60 AFLP primer combinations only 6 combinations viz. EcoR1-CT & Mse1-CG, EcoR1-GA & Mse1-CC, EcoR1-GT & Mse1-CG, EcoR1-TA & Mse1-CC, EcoR1-TC & Mse1-CG and EcoR1-TG & Mse1-CG were found better for generating polymorphisms. On the other hand, out of 19 SSR primers only 8 primers viz. ACA5, GCAGA3, GCAG4, GACG4, CAC5, GAC5, GCA5 and MR produced more polymorphisms in R. oryzae-sativae of which GACG4 was better as comparable with others. A dendogram, resulted from SSR-PCR using GACG4 primer, was constructed where 68 isolates of R. oryzae-sativae grouped into nine different fingerprinting types (FPTs) or cluster groups at 90% similarity level. Diverse populations of R. oryzae-sativae were suggested to use in screening for resistant genes and for selecting effective fungicide to manage aggregate sheath spot disease.

Keywords

Rice, Rhizoctoniaoryzae-sativae , SSR, AFLP, Genetic Diversity, Resistance

Reference

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