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Vol.3 , No. 3, Publication Date: Oct. 18, 2016, Page: 18-21
 on <i>In Vitro</i> Rooting of Sugarcane (<i>Saccharum officinarum</i> L.) Genotypes&description=Profuse rooting of <i>in vitro</i> grown shoots is an essential step for the establishment of the plantlets in the field, similarly hardening is a vital process prior to transplantation of rooted shoots to the soil. Hence, the experiment was conducted to optimize a protocol for profuse <i>in vitro</i> rooting of two sugarcane genotypes (B4906 and Pr1013). It was carried out in completely randomized design (CRD) with 2x5 factorial treatment arrangements of genotypes and NAA (2.0, 3.0, 4.0, 5.0 and 6.0 mgl<sup>-1</sup>) in combination. Separated shoots were cultured on ½ MS media supplemented with NAA. The results showed that the interaction effects of NAA and genotypes significantly influenced <i>in vitro</i> rooting. Half MS medium with 2 mgl<sup>-1</sup> NAA resulted in 91.67% rooted shoots with 12.58±0.23 roots and 2.54±0.04 cm root length in B4906 whereas 4 mgl<sup>-1</sup> resulted in 66.67% rooted shoots with 7.83±0.70 roots and 2.60±0.05 cm root length in Pr1013. Rooted plantlets acclimatized in greenhouse and 96.1% of plantlets survived successfully using soil, compost and sand (1:1:1) as potting mixture.&picture=http://www.aascit.org/aascit/decorators/img/journal/923.jpg)
| [1] | Belete Getnet, Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, Wonji, Ethiopia. |
| [2] | Kassahun Bantte, Department of Horticulture and Plant Science, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia. |
| [3] | Mulugeta Diro, Capacity Building for Scaling up of Evidence - Based Best Practices in Agricultural Production in Ethiopia (CASCAPE) Project, Addis Ababa, Ethiopia. |
Profuse rooting of in vitro grown shoots is an essential step for the establishment of the plantlets in the field, similarly hardening is a vital process prior to transplantation of rooted shoots to the soil. Hence, the experiment was conducted to optimize a protocol for profuse in vitro rooting of two sugarcane genotypes (B4906 and Pr1013). It was carried out in completely randomized design (CRD) with 2x5 factorial treatment arrangements of genotypes and NAA (2.0, 3.0, 4.0, 5.0 and 6.0 mgl-1) in combination. Separated shoots were cultured on ½ MS media supplemented with NAA. The results showed that the interaction effects of NAA and genotypes significantly influenced in vitro rooting. Half MS medium with 2 mgl-1 NAA resulted in 91.67% rooted shoots with 12.58±0.23 roots and 2.54±0.04 cm root length in B4906 whereas 4 mgl-1 resulted in 66.67% rooted shoots with 7.83±0.70 roots and 2.60±0.05 cm root length in Pr1013. Rooted plantlets acclimatized in greenhouse and 96.1% of plantlets survived successfully using soil, compost and sand (1:1:1) as potting mixture.
Keywords
Genotypes, NAA, Rooting, Acclimatization
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