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Vol.2 , No. 6, Publication Date: Jan. 4, 2016, Page: 140-144
 and aquaporin 3 (AQP3) are highly expressed in rat bladder endothelial and epithelial cells. Interestingly, <i>polyporus umbellate</i> effectively inhibits bladder carcinoma development in rats. In this study, we aim to examine how <i>polyporus umbellate</i> regulates AQP1 and AQP3 expression inbladder carcinoma model. Depending on the applied treatment, rats exhibiting bladder carcinoma were divided into the following five groups: saline (control), bladder carcinoma model, low dose (50 mg/kg), medium dose (250 mg/kg) and high dose (500 mg/kg) <i>polyporus umbellate</i>. Immunohistochemistry and RT-PCR were used to detect AQP1 and AQP3 expression after administration of <i>polyporus umbellate</i>. Bladder carcinoma was induced by N-butyl-n-(4-hydroxybutyl) nitrous amide (BBN). <i>Polyporus umbellate</i> inhibited bladder carcinoma development in rats. Compared with the model group, AQP1 and AQP3 protein expression decreased in <i>polyporus umbellate</i> treated rats. Moreover, with increasing dosage, ranging from250 to 500 mg/kg, <i>polyporus umbellate</i> decreased AQP1 and AQP3 mRNA expression (P<0.05, P<0.01, respectively). In conclusion，the <i>Polyporus umbellate</i> decreased bladder carcinoma growth and inhibited AQP1 and AQP3 expression.&picture=http://www.aascit.org/aascit/decorators/img/journal/923.jpg)
| [1] | Cheng Shao-yun, Clinical Laboratory, The Third People’s Hospital of Qingdao, Qingdao, Shandong Province, China. |
| [2] | Liu Zong-bao, Songshan Hospital of Qingdao University, Qingdao, Shandong Province, China. |
| [3] | Zhou Jing-yang, Beijing University of Chinese Medicine, Beijing, China. |
| [4] | Zhang Guo-wei, College of Chinese Medicine, Hebei University, Baoding, Hebei Province, China. |
Bladder carcinoma is a common malignancy. Aquaporin 1 (AQP1) and aquaporin 3 (AQP3) are highly expressed in rat bladder endothelial and epithelial cells. Interestingly, polyporus umbellate effectively inhibits bladder carcinoma development in rats. In this study, we aim to examine how polyporus umbellate regulates AQP1 and AQP3 expression inbladder carcinoma model. Depending on the applied treatment, rats exhibiting bladder carcinoma were divided into the following five groups: saline (control), bladder carcinoma model, low dose (50 mg/kg), medium dose (250 mg/kg) and high dose (500 mg/kg) polyporus umbellate. Immunohistochemistry and RT-PCR were used to detect AQP1 and AQP3 expression after administration of polyporus umbellate. Bladder carcinoma was induced by N-butyl-n-(4-hydroxybutyl) nitrous amide (BBN). Polyporus umbellate inhibited bladder carcinoma development in rats. Compared with the model group, AQP1 and AQP3 protein expression decreased in polyporus umbellate treated rats. Moreover, with increasing dosage, ranging from250 to 500 mg/kg, polyporus umbellate decreased AQP1 and AQP3 mRNA expression (P<0.05, P<0.01, respectively). In conclusion，the Polyporus umbellate decreased bladder carcinoma growth and inhibited AQP1 and AQP3 expression.
Keywords
Polyporus umbellate, Bladder Carcinoma, Aquaporin 1, Aquaporin 3
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