ISSN: 2375-3773
International Journal of Agricultural Sciences and Natural Resources  
Manuscript Information
 
 
Alleviation Drought Stress of Mungbean (Vigna radiata L.) Plants by using Arbuscular Mycorrhizal Fungi
International Journal of Agricultural Sciences and Natural Resources
Vol.1 , No. 1, Publication Date: Jul. 7, 2014, Page: 1-6
1871 Views Since July 7, 2014, 1106 Downloads Since Apr. 14, 2015
 
 
Authors
 
[1]    

Yagoob Habibzadeh, Agricultural Research Center of West Azarbaijan Province, Urmia- Iran.

[2]    

Ali Reza Evazi, Agricultural Research Center of West Azarbaijan Province, Urmia- Iran.

[3]    

Mahyar Abedi, Agricultural Research and Head of Vegetable and Irrigated Pulses Research Department Seed and Plant Improvement Institute, Karaj – Iran.

 
Abstract
 

In order to evaluate drought stress and arbuscular mycorrhiza on related root traits and grain yield of mungbean, a factorial experiment was carried out based on a randomized completely design in pot culture. Irrigation after 25, 50, 75, and 100 mm of evaporation from a Class A pan possessed irrigation’s levels as the first factor. At the second factor arranged Glomus mosseae, G. intraradices of mycorrhiza species and non-inoculum as a control with three replications were conducted in Urmia University in 2009. Results showed that grain yield of inoculated mungbean at both species with 4.29 and 4.31g/plant had the highest values. Both species had more root dry weight, root length and root to shoot ratio of dry weight than control. Root volume of inoculated mungbean with G. mosseae was not significant differences with control. Colonization of G. intraradices and G. mosseae, with 53.37 and 46.29% had the most values from plants irrigated after 25 mm of evaporation. At both species had the most leaf phosphorus with 260.2 and 258.3 mg/g of leaf dry weight. Mycorrhizae Colonization (r=0.73**), leaf phosphorus (r = 0.45**), root dry weight (r = 0.71**) and root volume (r = 0.68**) had positive correlation coefficients with grain yield. Variations of leaf proline accumulations from plants irrigated after 75 and 100 mm of evaporation were between 4.48 and 5.17 µmol/g leaf fresh weights. Although drought stress reduced grain yield, but inoculated it reduced the severity of stresses. Inoculated plants increased 65% of potential yield than control.


Keywords
 

Drought Stress, Grain Yield, Mungbean, Mycorrhiza, Root


Reference
 
[01]    

Abd-Alla, M. H., Omar, S. A. and Karanxha, S. 2000. The impact of pesticides on arbuscular mycorrhizal and nitrogen-fixing symbiosis in legumes. Appl Soil Ecol 14:191–200.

[02]    

Al-Karaki, G. N. and Al-Raddad, A. 1997. Effects of arbuscular mycorrhizal fungi and drought stress on growth and nutrient uptake of two wheat genotypes differing in drought resistance. Mycorrhiza 7:83–88.

[03]    

Al-Karaki, G. N. and Clark, R. B. 1998. Growth, mineral acquisition and water use by mycorrhizal wheat grown under water stress. J Plant Nutr 21:263–276.

[04]    

Al-Karaki, G. N., McMichael, B. and Zak, J. 2004. Field response of wheat to arbuscular mycorrhizal fungi and drought stress. Mycorrhiza 14:263–269.

[05]    

Allen, M. F., Smith, W. K., Moore, T. S. and Christensen, M. 1981. Comparative water relations and photosynthesis of mycorrhizal and non-mycorrhizal Bouteloua gracilis H.B.K. Lag ex Steud. New Phytol 88: 683–693.

[06]    

Auge, R. M. 2004. Arbuscular mycorrhizae and soil/plant water relations. Can J Soil Sci 84:373–381.

[07]    

Azcَn, R., Gomez, M. and Tobar, R. M. 1996. Physiological and nutritional responses by Lactuca sativa L. to nitrogen sources and mycorrhizal fungi under drought conditions. Biol Fertil Soils 22:156–161.

[08]    

Bates, L. S. , Waldren, R. P. and Teare, I. D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39: 205-207.

[09]    

Benami, A., and A. Ofen. 1984. Irrigation engineering—Sprinkler, trickle and surface irrigation: Principles, design and agricultural practices. Irrig. Eng. Sci. Publ., Haifa, Israel.

[10]    

Boomsma, C. R. and Vyn, T. J. 2008. Mize drought tolerance: potential importants through arbuscular mycorrhizal symbiosis. Field Crops Research 108: 14-31.

[11]    

Boureima, S., Diouf, M., Diop, T. A., Diatta, M., Leye, E. M., Ndiaye, F. and Seck, D. 2007. Effects of arbuscular mycorrhizal inocolation on the growth and the development of sesame (Sesamum indicum L.). African J Agri Res 3(3): 234-238.

[12]    

Demir, S. 2004. Influence of arbuscular mycorrhiza on some physiological‚ growth parameters of pepper. Turkish J Bio 28: 85-90.

[13]    

Giovannetti, M. and Mosse, B. 1980. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84:489–500.

[14]    

Graca, M. A. S., Barlocher, F. and Gessner, M. O. 2005. Methods to study litter decomposition: A practical guide. Springer-Verlag, Dordrecht, the Netherlands. 329 p.

[15]    

Goicoechea, N., Szalai, G., Antolon, M. C., Sonchez-Doaz, M. and Paldi, E. 1998. Influence of arbuscular mycorrhizae and Rhizobium on free polyamines and proline levels in water stressed alfalfa. J Plant Physiol153: 706–711.

[16]    

Habibzadeh, Y., Pirzad, A., Zardashti, M. R., Jalilian, J. and Eini, O. 2013. Effects of Arbuscular Mycorrhizal Fungi on Seed and Protein Yield under Water-Deficit Stress in MungBean. Agron J105:79–84.

[17]    

Huang, R. S., Smith, W. K. and Yost, R. S. 1985. Influence of vesicular-arbuscular mycorrhiza on growth, water relations, and leaf orientation in Leucaena leucocephala Lam. De Wit. New Phytol 99: 229–243.

[18]    

Faisal, E. A., Samia, O. Y., Elsiddig, A. E. E. 2000. Effects of Mycorrhizal inoculation and phosphorus application on the nodulation, mycorrhizal infection and yield components of Faba Bean grown under two different watering regimes. University of Khartoum J Agric Sci 8(2): 107-116.

[19]    

Kaya, C., Higgs, D., Kirnak, H. and Tas, I. 2003. Mycorrhizal colonization improves fruit yield and water use efficiency in Watermelon (Citrullus lanatus Thunb.) grown under well-watered and water-stressed conditions. Plant and Soil 253(2): 287-292.

[20]    

LU, J., LIU, M., Mao, Y. and Shen, L. 2007. Effects of vesicular-arbuscular mycorrhizae on the drought resistance of wild jujube (Zizyphs spinosus HU) seedlings. Front Agri China 1(4): 468-471.

[21]    

Marulanda, A., Porcel, R., Barea, M. and Azcon, R. 2007. Drought tolerance and antioxidant activities in laventies in lavender plants colonized by native drought-tolerant or drought-sensitive Glomus species. Micro Eco 54: 543-552.

[22]    

Ohnishi, T., Gall, R. S. and Mayer, M. L. 1975. An improved assay of inorganic phosphate in the presence of extralabile phosphate compounds: Application to the ATPase assay in the presence of phosphocreatine. Anal Biochem 69:261–267.

[23]    

Pelletier, S. and Dionne, J. 2004. Inoculation rate of Arbuscular Mycorrhizal Fungi Glomus intraradices and Glomus etunicatum affects establishment of landscape Turf with no irrigation or fertilizer inputs. Crop Sci 44: 335-338.

[24]    

Phillips, J. M., and Hayman, D. S. 1970. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapidassessment of infection. Trans Br Mycol Soc 55:158–161.

[25]    

Robert, M. 2001. Water relations, drought and vesicular arbuscular mycorrhizal symbiosis. Springer-Verlag. Mycorrhiza 11: 3-42.

[26]    

Ruiz-Lozano, J. M. 2003. Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress. New perspectives for molecular studies. Mycorrhiza 13: 309–317.

[27]    

Ruiz-Lozano, J. M., Azon, R. and Gomez, M. 1995. Effects of arbuscular- mycorrhizal Glomus species on drought tolerance: physiological and nutritional plant responses. App and Environ Micro 61: 456–460.

[28]    

Ruiz-Lozano, J. M., Gomez, M. and Azcَn, R. 1995. Influence of diff erent Glomus species on the time-course of physiological plant responses of lettuce to progressive drought stress periods. Plant Sci 110:37–44.

[29]    

Sanchez-Blanco, M. J., Ferrandez, T., Morales, M. A., Morte, A. and Alarcon, J. J. 2004. Variations in water status, gas exchange, and growth in Rosmarinus officinalis plants infected with Glomusdeserticola under drought conditions. J Plant Physiol 161:675–682.

[30]    

Sawers, R. J. H., Gutjahr, C. and Paszkowski, U. 2008. Cereal mycorrhiza: an ancient symbiosis in modern agriculture. Trend Plant Sci 13: 93–97.

[31]    

Smith, S. E. and Read, D. J. 2008. Mycorrhizal symbiosis. 3rd ed. Academic Press, London

[32]    

Sylvia, D. M. and Williams, S. E. 1992. Vesicular-arbuscular mycorrhizal and environmental stress. pp.101-124, In: mycorrhizae in sustainable agriculture. ASA Special Publication no. 54, ASA, CSSA, SSSA, Madison, WI.

[33]    

Watanabe, F. S. and Olsen, S. R. 1965. Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Sci Soc Am Proc 29:677–678.

[34]    

Wu, Q. and Xia, R. 2004. The relation between vesicular arbuscular mycorrhizae and water metabolism in plants. Chinese Agri Sci Bullet 20: 188-192.





 
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