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Vol.1 , No. 3, Publication Date: Sep. 25, 2014, Page: 28-31
 gastro-esophageal reflux disease and gastric ulceration duodenal ulcer, stress ulcer. A simple, efficient spectrophotometric method for the assay of famotidine has been developed for estimation of famotidine formulations. Comparision of three different brands of famotidine (nocid, famulcer and optifam) has also been done. The assay is carried out in lambda max at about 260 nm using water as solvent. Different formulations of drug was dissolved in water to prepare solutions containing famotidine 20 mg. Similarly, a sample of ground tablets of different brand were dissolved in water and dilutions were made in the range of 200-12.5 μgmL-1. The absorbance of sample preparation was measured at 260 nm against the water (blank solvent) and the assay was determined by the absorbance of each brand. Our results reveals that among all the three brands of famotidine (nocid, famulcer and optifam) famulcer shows highest percentage assay 111.44%. Calibration curves were linear over the range of 12.5-200 µg mL-1with a correlation coefficient ±0.92. Intra and inter-run precision and accuracy results were 98 to 102%. Proposed method was selective, accurate and precise therefore we can use for routine assay as well as quality control and clinical study.&picture=http://www.aascit.org/aascit/decorators/img/journal/922.jpg)
| [1] | Safila Naveed, Faculty of Pharmacy Jinnah University for women, Karachi, Pakistan. |
| [2] | Farya Zafar, Faculty of Pharmacy, Ziauddin University Karachi, Pakistan. |
Famotidine belongs to H2-receptor antagonist. It is used widely for the treatment of treatment of (GERD) gastro-esophageal reflux disease and gastric ulceration duodenal ulcer, stress ulcer. A simple, efficient spectrophotometric method for the assay of famotidine has been developed for estimation of famotidine formulations. Comparision of three different brands of famotidine (nocid, famulcer and optifam) has also been done. The assay is carried out in lambda max at about 260 nm using water as solvent. Different formulations of drug was dissolved in water to prepare solutions containing famotidine 20 mg. Similarly, a sample of ground tablets of different brand were dissolved in water and dilutions were made in the range of 200-12.5 μgmL-1. The absorbance of sample preparation was measured at 260 nm against the water (blank solvent) and the assay was determined by the absorbance of each brand. Our results reveals that among all the three brands of famotidine (nocid, famulcer and optifam) famulcer shows highest percentage assay 111.44%. Calibration curves were linear over the range of 12.5-200 µg mL-1with a correlation coefficient ±0.92. Intra and inter-run precision and accuracy results were 98 to 102%. Proposed method was selective, accurate and precise therefore we can use for routine assay as well as quality control and clinical study.
Keywords
Famotidine, Formulations, Spectrophotometry
Reference
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| [08] | Sultana N, Arayne MS and Safila Naveed and Shamshad Hina (2009) An RP-HPLC Method for Simultaneous Analysis and Interaction Studies on Enalapril Maleate and H2-Receptor Antagonists Acta Chromatographica 21(4),547–558.DOI:.1556/AChrom.21.2009.4.3 |
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| [11] | Huma Dilshad, Safila Naveed and Baqir Naqvi (2013) Assay of new formulations of isosorbide mononitrate by using uv spectrophotometer : BPJ0000115 - World Research Journal of Medicine Volume : 1 Issue : 1, pg9-10 |
| [12] | Huma Dilshad, Safila Naveed and Ghulam Sarwar (2014) Simple spectrophotometric assay of available brands of Acetaminophen tablets and their comparative study Journal of pharmacy and pharmaceutical sciences Volume 2, Issue 1,1-4 |
| [13] | Safila Naveed, Fatima Qamar, Ghulam Sarwer., (2014) Percentage assay of metformin in different medium using UV- spectrophotometer BPJ0000130 - World Research Journal of Organic Chemistry Volume : 2 Issue : 1, 12-14 ISSN: 2320-3374 & E-ISSN: 2320-5679, http://www.bioinfopublication.org/jouarchive.php?opt=&jouid=BPJ0000130 |
| [14] | Safila Naveed (2014) Simple UV spectrophotometric assay of Atorvastatin API formulation and their comparative study, Global Journal of Medical Research .14(2):35-38. https://globaljournals.org/GJMR_Volume14/4-Simple-UV-Spectrophotometric-Assay.pdf |
| [15] | Safila Naveed and Fatima Qamar (2014) A simple assay of Esomeprazole Using UV spectrophotometer The Global Journal of Pharmaceutical Research (TGJPR) 3(2); 1921-25, http://www.tgjpr.com/view-article.php?id=3433 |
| [16] | Safila Naveed and Fatima Qamar (2014) Simple UV spectrophotometric assay of Mefenamic acid International Journal of Pharma Sciences and Research (IJPSR) ,5(7):364-366 .http://www.ijpsr.info/ijpsr-v5n7.php |
| [17] | Safila Naveed, Shabana Naz Shah, Fatima Qamar , Nimra Waheed, and Safeena Nazeer, (2014) simple uv spectrophotometric assay of new formulation gentamycin J App Pharm Vol. 6; Issue 4: 407-410 |
| [18] | Safila Naveed, Shabana Naz Shah, Fatima Qamar , Nimra Waheed, and Safeena Nazeer, (2014) Simple UV spectrophotometric assay of Lincomycin, IJPRDD, 1 (2),10-12. |
| [19] | Safila Naveed* and Amber Nawab (2014) Assay of LVFX (levofloxacin) in different formulation by UV spectroscopy IJPRDD, 1 (2), 13-16. |
| [20] | Safila Naveed and Fatima Qamar (2014) Simple UV spectrophotometric assay of Metronidazole Open Access Library Journal, 1: e615:1-4. http://dx.doi.org/10.4236/oalib.1100615. |
