Vol.3 , No. 4, Publication Date: Jul. 19, 2016, Page: 40-45
[1] | Sawsan Mohammed Ali Hadi, Nutrition Research Institute, Baghdad, Iraq. |
Accurate laboratory testing requires an understanding of the interactions between collection tubes and blood specimens which can adversely affect the accuracy of laboratory test results. The blood collection tubes like plain tube (containing no gel) used for collection of serum for selected chemistry tests, and serum gel tube contains a clot activator and gel separator used for various laboratory tests. Gel blood collection tube is influenced by a number of variables, some of which are controlled by the tube manufacturer, tube material, centrifugation speed and temperature; separator gel may release materials that interfere with analytical assays. This study is an investigation of the effect of gel blood collection tubes on vitamin A retinol, 25-OH vitamin D assays by HPLC method.
Keywords
Blood Collection Tubes, Serum Separator Tubes (SST), Gel Tube, Vitamin A, Vitamin D
Reference
[01] | Becton Dickinson; BD Vacutainer Blood and Urine Collection- FAQs. www.bd.com/vacutainer/faqs/. |
[02] | Raffick A. R. Bowen, Glen L. Hortin, Gyorgy Csako, Oscar H. Otañez, Alan T. Remaley; Impact of blood collection devices on clinical chemistry assays, Clinical Biochemistry 2010; 43 4–25. http://www.avapezeshk.com/portalimages/publishimages/impact_of_blood_collection_devices_on_clinical_chemistry_assays.pdf |
[03] | Grace Mathew, Sara R. Zwart, Smith; Stability of blood analytes after storage in BD SST tubes for 12 mo, Clinically significant changes occurred only in 1,25-dihydroxyvitamin D and retinol-binding protein. Clinical Biochemistry, 2009; 42, 1732–1734. |
[04] | Bowen RA, Hortin GL, Csako G, Otañez OH, Remaley AT; Impact of blood collection devices on clinical chemistry assays. Clin Biochem. 2010; 43 (1-2): 4-25. http://www.ncbi.nlm.nih.gov/pubmed/19822139 |
[05] | RAR Bowen; Interferences www.ncbi.nlm.nih.gov/p.... National Center for Biotechnology Information. 2014. |
[06] | Ji SQ, Evenson MA. Effects of contaminants in blood-collection devices on measurement of therapeutic drugs. Clin Chem. 1983; 29: 456–61. |
[07] | Shi RZ, van Rossum HH, Bowen RA. Serum testosterone quantitation by liquid chromatography-tandem mass spectrometry: Interference from blood collection tubes. Clin Biochem. 2012; 45: 1706–9. |
[08] | A. Sujatha Rani; Confusions in Vitamin D Estimation and Interpretation. OSR Journal of Pharmacy and Biological Sciences. 2014; 9 (3): 14-20.www.iosrjournals.org |
[09] | Lensmeyer GL, Wiebe DA, Binkley B, Drezner MK; HPLC Method for 25-hydroxyvitamin D measurement: comparison with contemporary assay. Clin Chem 2006; 52: 1120-26. |
[10] | J. Gabler1, K. Lembright2, D. Payto2, Gel Separator in Blood Collection Tubes may Cause Interference with 25-Hydroxyvitamin D Measurement by Liquid Chromatography-Tandem Mass Spectrometry Method, American Association for Clinical Chemistry 2015. Poster Session. https://www.aacc.org/~/media/files/annual-meeting/2015/abstracts-2/aacc15_a127_a176.pdf?la=en |
[11] | Takayuki Ishige, Asuka Mochizuki1, Yoshio Kodera 3, 4, etc, Effects of Preanalytical Factors on Serum 25-hydroxyvitamin D3and 25-hydroxyvitamin D2 Measurements Using LC-MS/MS for the Clinical Laboratory Testing. 2013, Japan. https://www.msacl.org/2013_posters/201302052333_91710_poster_Ishige.pdf |
[12] | Yu S, Cheng X, Fang H; 25OHD analogues and vacuum blood collection tubes dramatically affect the accuracy of automated immunoassays. China Sci Rep. 2015; 5: 14636. http://www.ncbi.nlm.nih.gov/pubmed/26420221 |
[13] | Johannes M. W. van den Ouweland1, Stephan Church; High Total Protein Impairs Appropriate Gel Barrier Formation in BD Vacutainer Blood Collection Tubes Clinical Chemistry 2007. 53. 2 364-365 www.clinchem.org/content/53/2/364.full.pdf |
[14] | Evacuated Blood Collection System - Greiner Bio One, EIntended Use: VACUETTE® Blood Collection Tubes, Vitamin D3 determination by HPLC cannot be carried out with all gel tubes without some restrictions. https://www.gbo.com/.../980200_IFU_VenousBloodCollection_rev15 |
[15] | Elder, Peter A.; Lewis, John G.; King, Richard I.; Florkowski, Chris M, An anomalous result from gel tubes for vitamin D, Clin Chim Acta. 2009; 410 (1-2): 95. http://www.ncbi.nlm.nih.gov/pubmed/19804769 |
[16] | Raffick Bowen; Blood collection tube interference on clinical chemistry assays Troponin I, Cystatin C, Vitamin A and E, LDH. http://static.preanalytical-phase.org/zagreb 2013/06_Bowen. |
[17] | J. D. Buse, D. J. Orton, J. Boyd, H. Sadrzadeh; The influence of analytical interference introduced by separator gel blood collection tubes on steroid molecules analysis, Poster Session 2015. |
[18] | Raffick A. R. Bowen, Yung Chan, Joshua Cohen’ Effect of Blood Collection Tubes on Total Triiodothyronine and Other Laboratory Assays Clinical Chemistry 51: 2 424–433 (2005) |
[19] | Quest Diagnostics; Specimen Collection Tubes www.questdiagnostics.com/.../specimen_collection_tub” |
[20] | UC Irvine Medical Center, Department of Pathology, 101 The City Drive, Orange, CA 92868. http://www.pathology.uci.edu/services/specimen-containers.asp |
[21] | Eagle Biosciences, Inc. Catalog Number: VD331-H100. Using gel vials for blood collection might cause disturbed chromatograms caused http://www.eaglebio.com/content/VD331-H100.pdf |