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Vol.1 , No. 4, Publication Date: Sep. 16, 2014, Page: 58-67
. Materials and Methods. Investigation comprises 86 patients from 0 to 3 years of age with clinical and laboratory signs of IDA. 38 children-patients are of the University Hospital, Medical University – Pleven, Bulgaria – I group, and 48 are patients of the Sumy Regional Child’s Clinical Hospital, Sumy, Ukraine – II group. Comparison group includes 25 healthy children at the same age. Haematological parameters and the red cell indices were examined by analyzer MICROS – 18 (ABX). The serum erythropoietin (sEPO) and ferritin (sFR) levels were determined by ELISA. Serum content of trace elements was determined spectrophotometrically (I group) or by atomic absorption spectrophotometry (II group). To evaluate a relationship between markers of iron metabolism and haematological parameters we have used Spearman's correlation coefficients. Results. Formation of IDA is caused not only by violation of iron metabolism, but also other trace elements that directly or indirectly may affect both iron exchange and erythropoiesis regulation through the erythropoietin production. We found significant correlations between the level of chromium and transferrin saturation (r = -0.382, p = 0.018), zinc – with sEPO (r = 0.543, p = 0.036), copper – with sFR (r = -0.561, p = 0.029), and sEPO (r = -0.739, p = 0.0016), and cobalt – with sEPO (r = 0.769, p = 0.0021), that indicate the role of trace elements in the pathogenesis of IDA. Thus, if the routine therapy of IDA is ineffective, a concomitant micronutrient disorder should be considered, with an evaluation of trace elements level, and, if necessary, a correction should be carried out.&picture=http://www.aascit.org/aascit/decorators/img/journal/908.jpg)
| [1] | Andrii Nikolaevich Loboda, Department of Pediatrics with Medical Genetics, Sumy State University, Medical Institute, 31 Sanatornaya Street, Sumy 40007, Ukraine. |
| [2] | Maksym Vladimirovich Pogorielov, Department of Hygiene and Ecology, Sumy State University, Medical Institute, 31 Sanatornaya Street, Sumy 40007, Ukraine. |
| [3] | Pencho Tonchev, Department of Propaedeutics of Surgical Diseases, Medical University-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria. |
| [4] | Maria Angelova, Department of Chemistry and Biochemistry & Physics and Biophysics, Medical University-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria. |
| [5] | Tsvetelina Petkova-Marinova, Department of Physiology and Pathological Physiology, Medical University-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria. |
| [6] | Atanaska Bozhinova, Department of Chemistry and Biochemistry & Physics and Biophysics, Medical University-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria. |
| [7] | Vania Nedkova, Department of Pediatrics, University of Medicine-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria. |
Iron deficiency causes disbalance of other micro- and macroelements that leads to disruption of the exchange of most micronutrients and development of characteristic clinical symptoms. Interaction and correlation between trace elements and haematological parameters is still not clear. Aim. To investigate the relationship between haematological parameters, biochemical markers of iron metabolism and trace elements in children under 3 years with Iron-deficiency anaemia (IDA). Materials and Methods. Investigation comprises 86 patients from 0 to 3 years of age with clinical and laboratory signs of IDA. 38 children-patients are of the University Hospital, Medical University – Pleven, Bulgaria – I group, and 48 are patients of the Sumy Regional Child’s Clinical Hospital, Sumy, Ukraine – II group. Comparison group includes 25 healthy children at the same age. Haematological parameters and the red cell indices were examined by analyzer MICROS – 18 (ABX). The serum erythropoietin (sEPO) and ferritin (sFR) levels were determined by ELISA. Serum content of trace elements was determined spectrophotometrically (I group) or by atomic absorption spectrophotometry (II group). To evaluate a relationship between markers of iron metabolism and haematological parameters we have used Spearman's correlation coefficients. Results. Formation of IDA is caused not only by violation of iron metabolism, but also other trace elements that directly or indirectly may affect both iron exchange and erythropoiesis regulation through the erythropoietin production. We found significant correlations between the level of chromium and transferrin saturation (r = -0.382, p = 0.018), zinc – with sEPO (r = 0.543, p = 0.036), copper – with sFR (r = -0.561, p = 0.029), and sEPO (r = -0.739, p = 0.0016), and cobalt – with sEPO (r = 0.769, p = 0.0021), that indicate the role of trace elements in the pathogenesis of IDA. Thus, if the routine therapy of IDA is ineffective, a concomitant micronutrient disorder should be considered, with an evaluation of trace elements level, and, if necessary, a correction should be carried out.
Keywords
Iron Deficiency Anaemia, Haematological Parameters, Iron, Trace Elements
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