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Vol.4 , No. 4, Publication Date: Aug. 31, 2017, Page: 17-20
 on blood sugar and serum protein levels. Methods: Thirty-five (35) male Wistar rats were randomly allotted into seven (7) groups and each group had five rats. Each group was administered orally varying amounts of <i>Datura metel</i> for one week consecutively according to their body weights. The normal control (group1) received normal saline, groups two and three received leaf extract at low and high doses (300mg/kg bw and 600mg/kg bw) respectively. Groups four and five received seed extract at low and high doses respectively, while groups six and seven received fruit extract at low and high doses respectively. Blood samples were taken at the eighth day from the animals through the heart by cardiac puncture for biochemical analysis. Results: Generally, lower levels of blood sugar were observed in virtually all the groups when compared to the control (71.75±4.57mg/dl), however, group 2 had higher value (76.25±3.86 mg/dl) compared to the control. There were no significant differences (P˃0.05) among the groups in the levels of protein, albumin and globulins. Lower values were observed for globulins and total protein levels within treatments when high doses of the extracts (leaf, seed and fruits) were administered to the rats. However, the highest level of globulin was recorded for normal control as 30.85±3.75g/l, whereas the highest level of protein was obtained in group 4 (62.60± 3.63). Conclusion: The study showed that there were no deleterious effects on the administration of <i>Datura metel</i> on the blood/serum biochemical parameters analysed. Recommendation: Further work should be carried out on Datura metel with the view of revealing more information on its nutritional effects and metabolomics.&picture=http://www.aascit.org/aascit/decorators/img/journal/908.jpg)
| [1] | Arowora Kayode Adebisi, Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Nigeria. |
| [2] | Imo Chinedu, Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Nigeria. |
| [3] | Ezeonu Chukwuma Stephen, Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Nigeria. |
| [4] | Isa Abubakar Bawa, Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Nigeria. |
Purpose: This study investigated the effects of orally administered extracts of Datura metel (leaf, seed and fruit) on blood sugar and serum protein levels. Methods: Thirty-five (35) male Wistar rats were randomly allotted into seven (7) groups and each group had five rats. Each group was administered orally varying amounts of Datura metel for one week consecutively according to their body weights. The normal control (group1) received normal saline, groups two and three received leaf extract at low and high doses (300mg/kg bw and 600mg/kg bw) respectively. Groups four and five received seed extract at low and high doses respectively, while groups six and seven received fruit extract at low and high doses respectively. Blood samples were taken at the eighth day from the animals through the heart by cardiac puncture for biochemical analysis. Results: Generally, lower levels of blood sugar were observed in virtually all the groups when compared to the control (71.75±4.57mg/dl), however, group 2 had higher value (76.25±3.86 mg/dl) compared to the control. There were no significant differences (P˃0.05) among the groups in the levels of protein, albumin and globulins. Lower values were observed for globulins and total protein levels within treatments when high doses of the extracts (leaf, seed and fruits) were administered to the rats. However, the highest level of globulin was recorded for normal control as 30.85±3.75g/l, whereas the highest level of protein was obtained in group 4 (62.60± 3.63). Conclusion: The study showed that there were no deleterious effects on the administration of Datura metel on the blood/serum biochemical parameters analysed. Recommendation: Further work should be carried out on Datura metel with the view of revealing more information on its nutritional effects and metabolomics.
Keywords
Biochemical Analysis, Blood Sugar, Ethanolic Extract, Protein Levels, Serum, Wistar Rats
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