AASCIT - Journal - All Issues

ISSN: 2375-379X

Health Sciences Research

The Journal

Manuscript Information

Protective Effects of Ethanolic Leaf Extract of Gongronema latifolium on Antioxidant Indices of Acetaminophen-Induced Toxicity in Albino Rats

Health Sciences Research
Vol.4 , No. 2, Publication Date: Aug. 3, 2017, Page: 6-9

286 Views Since August 3, 2017, 825 Downloads Since Aug. 3, 2017

Paper in PDF (230K)

Follow on

Authors

[1]	Chinedu Imo, Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Nigeria.

Abstract

This study examined the protective effects of ethanolic leaf extract of Gongronema latifolium on selected antioxidant indices of acetaminophen-induced toxicity in albino rats. Fifty healthy male albino rats were used in this study and were randomly placed into five groups with ten rats in each group. The leaf extract was administered accordingly to groups 3, 4 and 5 through gavage intubation. At the end of the administration of the extracts, blood was collected by cardiac puncture for biochemical analysis. The results show that antioxidant enzymes: Catalase, SOD, GST and GSH reduced, while MDA increased in the group administered acetaminophen only, but the antioxidant enzymes increased, while MDA decreased in all groups administered the leaf extract. Catalase and SOD increased from 2.35 ± 0.12 to 6.86 ± 0.29 µmol/mg protein and 1.46 ± 0.09 to 3.59 ± 0.23 µmol/ml respectively, while GST and GSH increased from 0.40 ± 0.02 to 0.97 ± 0.04 µmol/ml and 0.61 ± 0.02 to 1.64 ± 0.02 µmol/ml respectively. MDA reduced from 4.64 ± 0.33 to 1.57 ± 0.23 µmol/ml. These results indicate that the leaf extract of Gongronema latifolium has protective effect on the indices evaluated and can be used as an antioxidant agent against the toxicity induced.

Keywords

Antioxidant, Enzymes, Free Radicals, Gongronema latifolium, Oxidative Stress

Reference

[01]	Deepa, M. and Renuka, D. (2014). Potential Anti-Inflammatory Medicinal Plants- A Review. Int. J. Pharm. and Pharmaceutical Sci., 6 (4): 43-49.
[02]	Tiwari, A. K. and Roa, M. (2002). Diabetes mellitus and multiple therapeutic approaches of phytochemical. Present status and future prospects. Curr. Sci., 83: 30-38.
[03]	Ugochukwu, N. H., Babady, N. E., Cobourne, M. and Gasset, S. R. (2003). The effect of Gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J. Biosci., 20 (1): 1-5.
[04]	Imo, C., Uhegbu, F. O., Imo, C. K. and Ifeanacho, N. G. (2013). Ameliorating effect and haematological activities of methanolic leaf extract of Gongronema latifolium in acetaminophen-induced hepatic toxicity in wistar albino rats. International Journal of Biosciences, 3 (11): 183-188.
[05]	Nwanjo, H. U. and Alumanah, E. O. (2005). Effect of aqueous extract of Gongronema latifolium leaf on some indices of liver function in rats. Global J. Med. Sci., 4 (1): 29-32.
[06]	Imo, C. and Uhegbu, F. O. (2015). Phytochemical Analysis of Gongronema latifolium Benth Leaf Using Gas Chromatographic Flame Ionization Detector. International Journal of Chemical and Biomolecular Science, 1 (2): 60-68.
[07]	Egbung, G. E., Atangwho, I. J., Iwara, I. A. and Eyong, U. E. (2011). Micronutrient and phytochemical composition of root bark and twig extracts of Gongronema latifolium. J. Med. and Medical Sci., 2 (11): 1185-1188.
[08]	Araujo, A. S. R., Ribeiro, M. F. M., Enzveiler, A., Schenkel, P., Fernandes, T. R. G., Partata, W. A., Iriqoyen, M. C., Llesuy, S. and Belló-klein, A. (2006). Myocardial antioxidant enzyme activities and concentration and glutathione metabolism in experimental hyperthyroidism. Mol. Cell Endocrinol., 249: 133-139.
[09]	Onkawa, H., Ohishi, N. and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351–358.
[10]	Ellman, G. L. (1959). Tissue Sulfhydryl Groups. Arch. Biochem. Biophys., 82: 70–77.
[11]	Habig, W. H., Pabst, M. J., Fleischner, G., Gatmaitan, Z., Arias, I. M. and Jakoby, W. B. (1974). The Identity of Glutathione S-Transferase B with Ligandin, a Major Binding Protein of Liver. Proc. Natl. Acad. Sci. U.S.A., 71 (10): 3879–3882.
[12]	Ogbunugafor, H., Sofidiya, O. and Okpuzor, J. (2010). Effect of Extracts of Hymenocardia acida Tul (Hymenocardiaceae) on rats. J Am. Sci., 6: 143-146.
[13]	Sinha, A. K. (1972). Colourimetric assay of catalase. Anal. Biochem., 47: 389-394.
[14]	Okpala, J. C., Sani, I., Abdullahi, R., Ifedilichukwu, H. N. and Igwe, J. C. (2014). Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in CCl₄^- induced oxidative damage in Wistar albino rats. Afri. J. Biochem. Res., 8 (2): 52-64.
[15]	Mohajeri, D., Amouoghli, T., Bahram, D., Yousef, N. M. (2011). Protective effect of Turnip Root (Brassica Rapa. L) ethanolic extract on early hepatic injury in alloxanized diabetic Rats. Aust. J. Basic Appl. Sci., 5 (7): 748-756.
[16]	Tamilarasi, R., Sivanesan, D. and Kanimozhi, P. (2012). Hepatoprotective and antioxidant efficacy of Anethum graveolens linn in carbon tetrachloride-induced hepatotoxicity in albino rats. J. Chem. Pharm. Res., 4 (4): 1885-1888.
[17]	Kim, H. Y., Kim, J. K., Choi, J. H., Jung, J. Y., Oh, W. Y., Kim, D. C., Lee, H. S., Kim, Y. S., Kang, S. S., Lee, S. H. and Lee, S. M. (2010). Hepatoprotective effect of pinoresinol on carbon tetrachloride-induced hepatic damage in mice. J. Pharmacol. Sci., 112 (1): 105-112.
[18]	Khan, M. R., Marium, A., Shabbir, M., Saeed, N. and Bokhari, J. (2012). Antioxidant and hepatoprotective effects of Oxalis corniculata against carbon tetrachloride (CCl₄) induced injuries in rats. Afr. J. Pharm. Pharmacol., 6 (30): 2255-2267.