ISSN: 2375-3838
International Journal of Clinical Medicine Research  
Manuscript Information
 
 
Influence of α-Asarone on Human Esophageal Carcinoma Cells and Its Molecular Mechanisms
International Journal of Clinical Medicine Research
Vol.5 , No. 4, Publication Date: Jun. 28, 2018, Page: 97-102
404 Views Since June 28, 2018, 123 Downloads Since Jun. 28, 2018
 
 
Authors
 
[1]    

Baiyan Wang, Key Discipline Laboratory of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China.

[2]    

Qianqian Han, Key Discipline Laboratory of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China.

[3]    

Huiru Zhou, The Second School of Clinical Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China.

[4]    

Ruiqin Li, Scientific Research Pathological Experiment Center, Henan University of Traditional Chinese Medicine, Zhengzhou, China.

[5]    

Yanqin Zhu, Key Discipline Laboratory of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China.

 
Abstract
 

α-Asarone - the main component of the rhizomes of Acori graminei that has been traditionally used in the treatment of nervous system disorders. The present work envisages an investigation of the influence of α-asarone on human esophageal carcinoma cells (Eca-109), explore its molecular mechanism, and provide an experimental basis for the clinical application. The methyl thiazolyl tetrazolium (MTT) assay was performed to detect the cell proliferation after cells were processed with α-asarone for various time points. The morphological changes of the apoptotic cells stained with AO/EB were observed with an inverted microscope. After 48 hours of in vitro culturing, the expression levels of GRP78, CHOP, caspase-3 and caspase-9 genes and proteins were measured by RT-PCR and Western blot technique. MTT data showed that α-asarone inhibited the cell proliferation in a time and dose-dependent manner. In addition, α-asarone induced apoptosis in the Eca-109 cells and increased the expressions of GRP78, CHOP, caspase-3 and caspase-9 significantly. The present study demonstrated that the apoptosis was mediated by an up-regulation of expressions of the endoplasmic reticulum-associated GRP78, CHOP, and cysteine proteinase caspase-3, caspase-9. This provides an experimental basis for clinical application of α-asarone in anti-tumor therapy, however further investigations of the in vivo experimentation need to be done.


Keywords
 

α-Asarone, Apoptosis, GRP78, CHOP, Caspase-3, Caspase-9


Reference
 
[01]    

Yan Zhang, Shan Li, Hang He, Qianqian Han, Baiyan Wang & Yanqin Zhu. Influence of Tanshinone IIA on apoptosis of human esophageal carcinoma Eca-109 cells and its molecular mechanism. Thoracic Cancer. 2017; (8): 296–303.

[02]    

Jing Wang, Zi-Rong Yang, Wei-Guo Dong, etc. Cooperative inhibitory effect of sinomenine combined with 5-fluorouracil on esophageal carcinoma. 2013; 19 (45): 8292-8300.

[03]    

Cheng Ch, Hai Zh, Zhen W, Shao-Ping L. Matrine induces apoptosis of human colon cancer HT29 cells through mitochondrial pathway. Chinese Journal of Integrated and Western Medicine on Digestion. 2013; 12, 628-631.

[04]    

Jung-Won Shin, Young-Jin Cheong, Yong-Mo Koo, Sooyong Kim, Chung-Ku Noh, Young-Ha Son, Chulhun Kang and Nak-Won Sohn. a-Asarone Ameliorates Memory Deficit in Lipopolysaccharide-Treated Mice via Suppression of Pro-Inflammatory Cytokines and Microglial Activation. Biomolecules Therapeutics. 2014; 22 (1), 17-26.

[05]    

Hye-Jung Park, Soo-Jin Lee, Moon-Moo Kim. Effect of alpha-asarone on angiogenesis and matrix metalloproteinase. Environmental toxicology and pharmacology. 2015; 39: 1107-1114.

[06]    

Tian-Tian W, Fang W, Yan-Qin Zh, Qiang F, Ai-She G. Experimental Study on α-asarone Induce Apoptosis in Human Esophagral Carcinoma Eca-109 Cell Line. China journal of Chinese medicine. 2014; 29 (4): 473-475.

[07]    

Yi-Zi Zh, Zhi-Guang C, Xin H, Zhi-Ming Sh. The endoplasmic reticulum stress markers GRP78 and CHOP predict disease-free survival and responsiveness to chemotherapy in breast cancer. Breast Cancer Research Treat. 2014; 145: 349–358.

[08]    

Qiu-Di L, Xi-Xia L, Yuan W, Ming-Hui T, Jie-Mei Y, Xing W, Jin-Shan H, Si-Jun L. An effect study of α-asarone on the expression of Bax and Bcl-2 in hippocampal neurons of epilepsy rats. Journal of Apoplexy and Nervous Diseases. 2013; 30 (4): 312-315.

[09]    

Xi Z, Shen-Lin L, Jin-Yong Zh, Jian W, Bo-Fan L, Rui-Ping W. Beta-asarone Induces LoVo Colon Cancer Cell Apoptosis by Up-regulation of Caspases through a Mitochondrial Pathway in vitro and in vivo. Asian Pacific Journal of Cancer Prevention. 2012; 13: 5291-5298.

[10]    

Guang-hua Lu, Yu-jie Li, Yan-qin Zhu. Experimental study of the inhibitory effect of α-As-arone on the Ec-109 cell in vitro. Chinese Pharmacological Bulletin. 2012; 28 (1): 148.

[11]    

Lei Y, Jian-Shuang L, Zhi-Yun Ch, Mao-Xiang Yan, Bei-Hui H. The role of ginkgo biloba extract on the hepatic GRP78 and CHOP expression in rats with liver fibrosis. Chinese clinical pharmacology and therapeutics. 2014; 19 (4): 377-380.

[12]    

Wei C, Jie M, Xing-Qin W, Wen-Juan Y, Jing Zh, Qiu-He J. Free Fatty Acid Induces Endoplasmic Reticulum Stress and Apoptosis of b-cells by Ca2+/Calpain-2 Pathways. 2013; PLoS ONE 8 (3): e59921. doi: 10.1371/journal.pone.0059921.

[13]    

Bi-Liu W, Guo-Ping L, Ze-Jin P, Guan-You H, Jia-Lin Feng, Yan-Qing Y, Ling-Fei W. Effect of adenosine on EC109 cell apoptosis and its endoplasmic reticulum molecular mechanism. Chinese Journal of Pharmacology and Toxicology. 2011; 25 (6): 532-537.

[14]    

Jian M, Yu Q, Lan Y, Liang P, Zheng X, Li-Na H, Chao F, Hong Q, Hong-Zhuan Ch. Desipramine induces apoptosis in rat glioma cells viaendoplasmic reticulum stress-dependent CHOP pathway. Journal of Neurooncol. 2011; 101 (1): 41-48.

[15]    

Mina Thon, Toru Hosoi, Michiko Yoshii, Koichiro Ozawa. Leptin induced GRP78 expression through the PI3K-mTOR pathway in neuronal cells. Scientific Reports. 2014; 4: 1-8.

[16]    

Miao W, Tao H, Fu-Qing Z, Guo-Song J, Liang W, Li-Duan Zh, Qiang-Song T. Effect of smac on TRAIL-induced apoptosis of prostate cancer cell line PC-3 and the molecular mechanism. Journal of hua zhong science technology university. 2012; 32 (2): 233-236.

[17]    

Zheng-Qi F, Hong-Yan Zh, Li-Jiang L. Central role of GRP78 in growth of gastric carcinoma cells. Chinese Journal of Pathophysiology. 2014; 30 (4): 625-628.

[18]    

Yi-Guo W, Chang-Hong L, Sen H, Peng-Ju Zh, Qian L. Effect of recombinant Hepatitis B virus on human glomerular mesangial cell apoptosis. Biotechnology & Biotechnological Equipment. 2014; 28: 4, 689-696, DOI: 10.1080/13102818.2014.948278.

[19]    

He-Ming Y, Lu-Qing L, Wen-Juan W, Xiao-Ning W, Lian-Qiu M. Expression and significance of GRP78, CHOP, caspase-3 and caspase-9 proteins in preconditioning focal cerebral ischemia in rats. Journal of Apoplexy and Nervous Diseases. 2012; 29 (8): 703-706.

[20]    

Jian W, Xing-Xing Zh, Qing-Ming S, Min Ch, Sheng-Lin L, Xiang Zh, Jin-Yong Zh, Xi Z β-asarone inhibits gastric cancer cell proliferation. Oncology Reports. 2015; 34 (6): 3043-3050.





 
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