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Vol.5 , No. 4, Publication Date: Jun. 28, 2018, Page: 97-102
, explore its molecular mechanism, and provide an experimental basis for the clinical application. The methyl thiazolyl tetrazolium (MTT) assay was performed to detect the cell proliferation after cells were processed with α-asarone for various time points. The morphological changes of the apoptotic cells stained with AO/EB were observed with an inverted microscope. After 48 hours of <i>in vitro</i> culturing, the expression levels of GRP78, CHOP, caspase-3 and caspase-9 genes and proteins were measured by RT-PCR and Western blot technique. MTT data showed that α-asarone inhibited the cell proliferation in a time and dose-dependent manner. In addition, α-asarone induced apoptosis in the Eca-109 cells and increased the expressions of GRP78, CHOP, caspase-3 and caspase-9 significantly. The present study demonstrated that the apoptosis was mediated by an up-regulation of expressions of the endoplasmic reticulum-associated GRP78, CHOP, and cysteine proteinase caspase-3, caspase-9. This provides an experimental basis for clinical application of α-asarone in anti-tumor therapy, however further investigations of the <i>in vivo</i> experimentation need to be done.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Baiyan Wang, Key Discipline Laboratory of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China. |
| [2] | Qianqian Han, Key Discipline Laboratory of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China. |
| [3] | Huiru Zhou, The Second School of Clinical Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China. |
| [4] | Ruiqin Li, Scientific Research Pathological Experiment Center, Henan University of Traditional Chinese Medicine, Zhengzhou, China. |
| [5] | Yanqin Zhu, Key Discipline Laboratory of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China. |
α-Asarone - the main component of the rhizomes of Acori graminei that has been traditionally used in the treatment of nervous system disorders. The present work envisages an investigation of the influence of α-asarone on human esophageal carcinoma cells (Eca-109), explore its molecular mechanism, and provide an experimental basis for the clinical application. The methyl thiazolyl tetrazolium (MTT) assay was performed to detect the cell proliferation after cells were processed with α-asarone for various time points. The morphological changes of the apoptotic cells stained with AO/EB were observed with an inverted microscope. After 48 hours of in vitro culturing, the expression levels of GRP78, CHOP, caspase-3 and caspase-9 genes and proteins were measured by RT-PCR and Western blot technique. MTT data showed that α-asarone inhibited the cell proliferation in a time and dose-dependent manner. In addition, α-asarone induced apoptosis in the Eca-109 cells and increased the expressions of GRP78, CHOP, caspase-3 and caspase-9 significantly. The present study demonstrated that the apoptosis was mediated by an up-regulation of expressions of the endoplasmic reticulum-associated GRP78, CHOP, and cysteine proteinase caspase-3, caspase-9. This provides an experimental basis for clinical application of α-asarone in anti-tumor therapy, however further investigations of the in vivo experimentation need to be done.
Keywords
α-Asarone, Apoptosis, GRP78, CHOP, Caspase-3, Caspase-9
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