ISSN: 2375-3838
International Journal of Clinical Medicine Research  
Manuscript Information
Tunisian Pistacia lentiscus Fruit Oil: Biochemical Composition and Wound Healing Activity in a Rat Model
International Journal of Clinical Medicine Research
Vol.5 , No. 4, Publication Date: Jun. 1, 2018, Page: 72-85
905 Views Since June 1, 2018, 433 Downloads Since Jun. 1, 2018

Sameh Ben Khedir, Laboratory of Histology, Embryology, and Reproductive Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.


Sana Bardaa, Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.


Dorsaf Moalla, Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.


Zohra Ghlissi, Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.


Zouheir Sahnoun, Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.


Tarek Rebai, Laboratory of Histology, Embryology, and Reproductive Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.


The oil extracted from Pistacia lentiscus fruit has long been used for wound healing in traditional medicine. The present study aimed to characterize an oil extract from Tunisian Pistacia L. fruit and to elucidate on the relationship between its chemical compositions and wound healing properties. The quality indices and composition and content of certain bioactive constituents of the cold pressed oil obtained from Pistacia lentiscus fruits were analyzed and studied for their wound healing properties. Uniform full thickness excision wounds were induced on the dorsum of 72 rats, randomly divided into three groups. The wounds were photographed, and topically treated with saline solution (control group), 0.13 mg/mm2 of a reference drug (“Cicaflora cream®”), and 0.52 μl/mm2 of Pistacia lentiscus fruit oil (PLFO). The results showed an excellent quality of PLFO with High content of monoterpens and sesquiterpens. The fatty acids were dominated by Oleic acid, mono-unsaturated fatty acid, with an amount of 45.66%. The sterol content analysis revealed the prevalence of β-Sitosterol, accounting for 77.94%. The results from morphometric assessment and histological findings revealed that best wound healing activity, with reappearance of skin appendages and well organized collagen fibers without inflammatory cells, was exhibited by PLFO, followed by «CICAFLORA»®. Overall, the findings indicated that P. lentiscus fruit oil has a number of promising wound-healing properties that make it a strong candidate for application in human therapy.


Pistacia lentiscus L., Fruit Oil, Acidity, Peroxide Value, Fatty Acids, Sterols, Wound Healing, Histological Analysis


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