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Vol.5 , No. 2, Publication Date: Apr. 10, 2018, Page: 20-26
 is a non-selective phosphodiesterase inhibitor which have role in prevention of oxidative stress, inflammation and fibrosis which ultimately affect the liver. The major goal of the study was to evaluate the role of PTX on carbon tetrachloride (CCl<sub>4</sub>) induced acute liver injury model and its possible mechanisms in mice. Male C57BL/6 mice were divided into four groups: control, PTX, CCl<sub>4</sub> and PTX+ CCl<sub>4</sub> treated groups. Mice were administrated CCl<sub>4</sub> together with or without PTX for a week and sacrificed 72 hour after the last injection of CCl<sub>4</sub> and PTX. Histopathological evaluation was performed. The liver function test, indices of oxidative stress including NADPH oxidase (NOX) and cytochrome P450 2E1 (CYP2E1) enzyme activity, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) levels in liver tissues were measured. mRNA expression of different pro inflammatory cytokines and protein expression of nuclear factor-κB (NF-Kb) were checked. PTX significantly attenuated CCl<sub>4</sub>-induced liver injury histopathologically and improves the liver function. PTX also remarkably suppressed the secretions of pro-inflammatory cytokines and translocation of the p65 subunits of NF-κB to nucleus induced by CCl<sub>4</sub>. In addition PTX can also reduce the generation of oxidative stress by decreasing the enzyme activity of NOX, CYP2E1 and the levels of MDA and also by increasing the cellular anti-oxidant GSH and SOD. In conclusion, PTX ameliorated the effects of CCl<sub>4</sub> induced acute liver injury in mice by inhib¬iting oxidative stress, expression of pro-inflammatory cytokines and NF-κB activation.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Bidhan Chandra Chakraborty, Centre for Liver Research, School of Digestive & Liver Diseases, Institute of Post Graduate Medical Education & Research, Kolkata, India. |
| [2] | Debasree Bishnu, Centre for Liver Research, School of Digestive & Liver Diseases, Institute of Post Graduate Medical Education & Research, Kolkata, India. |
| [3] | Suman Santra, Centre for Liver Research, School of Digestive & Liver Diseases, Institute of Post Graduate Medical Education & Research, Kolkata, India. |
| [4] | Gopal Krishna Dhali, Centre for Liver Research, School of Digestive & Liver Diseases, Institute of Post Graduate Medical Education & Research, Kolkata, India. |
| [5] | Abhijit Chowdhury, Centre for Liver Research, School of Digestive & Liver Diseases, Institute of Post Graduate Medical Education & Research, Kolkata, India; Indian Institute of Liver and Digestive Sciences, Kolkata, India. |
| [6] | Amal Santra, Centre for Liver Research, School of Digestive & Liver Diseases, Institute of Post Graduate Medical Education & Research, Kolkata, India; Indian Institute of Liver and Digestive Sciences, Kolkata, India. |
Pentoxifylline (PTX) is a non-selective phosphodiesterase inhibitor which have role in prevention of oxidative stress, inflammation and fibrosis which ultimately affect the liver. The major goal of the study was to evaluate the role of PTX on carbon tetrachloride (CCl4) induced acute liver injury model and its possible mechanisms in mice. Male C57BL/6 mice were divided into four groups: control, PTX, CCl4 and PTX+ CCl4 treated groups. Mice were administrated CCl4 together with or without PTX for a week and sacrificed 72 hour after the last injection of CCl4 and PTX. Histopathological evaluation was performed. The liver function test, indices of oxidative stress including NADPH oxidase (NOX) and cytochrome P450 2E1 (CYP2E1) enzyme activity, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) levels in liver tissues were measured. mRNA expression of different pro inflammatory cytokines and protein expression of nuclear factor-κB (NF-Kb) were checked. PTX significantly attenuated CCl4-induced liver injury histopathologically and improves the liver function. PTX also remarkably suppressed the secretions of pro-inflammatory cytokines and translocation of the p65 subunits of NF-κB to nucleus induced by CCl4. In addition PTX can also reduce the generation of oxidative stress by decreasing the enzyme activity of NOX, CYP2E1 and the levels of MDA and also by increasing the cellular anti-oxidant GSH and SOD. In conclusion, PTX ameliorated the effects of CCl4 induced acute liver injury in mice by inhib¬iting oxidative stress, expression of pro-inflammatory cytokines and NF-κB activation.
Keywords
Acute Liver Injury, Oxidative Stress, Pentoxifilline
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