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Vol.1 , No. 3, Publication Date: Aug. 7, 2014, Page: 72-80
 stimulated with synthetic Pam2. This present study indicates that the binding of Pam2 and TLR2/6 directly triggers hepcidin expression in macrophages. This shows that hepcidin can be produced in the local environment of bacterial infection, therefore playing an essential role in the innate immune system. This is important because the pathogen producing the inflammation can be killed quickly via iron withdrawal, without inflammatory cytokines having to reach the liver first. These result indicates that the iron regulatory hormone and antimicrobial peptide, hepcidin, can potentially represent a host defence mechanism against intracellular pathogens and provides hope for new chemotherapies against intracellular diseases. There is need for further studies to investigate the potential development of new chemotherapies that takes into consideration the potential antimicrobial effect of the peptide, hepcidin in the management of intracellular pathogens.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Abdulrahaman Y., Department of Haematology, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| [2] | Yeldu M. H., Department of Clinical Chemistry Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| [3] | Dallatu M. K., Department of Clinical Chemistry Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| [4] | Erhabor O., Department of Haematology, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
Hepcidin plays a central role in orchestration on iron metabolism also providing a link between iron metabolism, inflammation and innate immunity. In this present study, we investigated the intrinsic ability of THP-1cell which have been differentiated to macrophages to expressed hepcidin in-vitro and involvement of pattern recognition by toll-like receptor (TLR6/2) stimulated with synthetic Pam2. This present study indicates that the binding of Pam2 and TLR2/6 directly triggers hepcidin expression in macrophages. This shows that hepcidin can be produced in the local environment of bacterial infection, therefore playing an essential role in the innate immune system. This is important because the pathogen producing the inflammation can be killed quickly via iron withdrawal, without inflammatory cytokines having to reach the liver first. These result indicates that the iron regulatory hormone and antimicrobial peptide, hepcidin, can potentially represent a host defence mechanism against intracellular pathogens and provides hope for new chemotherapies against intracellular diseases. There is need for further studies to investigate the potential development of new chemotherapies that takes into consideration the potential antimicrobial effect of the peptide, hepcidin in the management of intracellular pathogens.
Keywords
Hepcidin, THP-1cells, Pam2, TLR2/6
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