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Vol.4 , No. 6, Publication Date: Nov. 25, 2017, Page: 76-82
 cell count which is also used as a parameter in health and immune status in the management of HIV-Infected patients on antiretroviral therapy (ART). Higher CD4 counts typically signify healthier immune systems. However, IL-6 has a multiple biological effects on immune and proinflammatory responses including enhancing and proliferation of and maturation of B cells as well as immunoglobulin production, and to activate T lymphocyte function. In this descriptive case-control prospective study, blood samples of selected HIV-infected patients on ART were collected during one of their periodic visit. Those with CD4 count of 100 to 200 cells/ul and those with ≥ 400 cells/ul blood were tested for Interleukin-6 level along with healthy controls by cytoflow and ELISA technique respectively. Results revealed IL-6 levels of 272.93±250.84 in those with CD4 count of 100 to 200 cells/ul, while 301.99±261.23 was obtained in patients with ≥ 400 cells/ul, while the apparently healthy controls have 145.98±156.36 within the standard range of 6.5 – 200 pg/ml. There was significant increase in the IL-6 levels when compared to the healthy controls. In HIV-Infected patients on ART, the higher the CD4 count, the higher the IL-6 cytokine level with the females generally having higher IL-6 cytokine values than the males.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Bamidele Soji Oderinde, Department of Medical Laboratory Science, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria; WHO National Polio / ITD Laboratory, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. |
| [2] | Jessy Thomas Medugu, Department of Medical Laboratory Science, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria. |
| [3] | Olajide Olubunmi Agbede, Department of Medical Microbiology and Parasitology, College of Health Sciences, University of Ilorin, Ilorin, Nigeria. |
| [4] | Peter Elisha Ghamba, WHO National Polio / ITD Laboratory, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. |
| [5] | Abosede Florence Oderinde, PEPFAR / APIN Clinic, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. |
| [6] | Babajide Bamidele Ajayi, Department of Immunology, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. |
| [7] | Saka Saheed Baba, Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria. |
| [8] | Ngamarju Apagu Satumari, WHO National Polio / ITD Laboratory, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. |
| [9] | James Mshelia Usman, Department of Immunology, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. |
Immunity status of HIV-Infected patients is physiologically compromised which can be inferred from the Cluster of differentiation four (CD4+) cell count which is also used as a parameter in health and immune status in the management of HIV-Infected patients on antiretroviral therapy (ART). Higher CD4 counts typically signify healthier immune systems. However, IL-6 has a multiple biological effects on immune and proinflammatory responses including enhancing and proliferation of and maturation of B cells as well as immunoglobulin production, and to activate T lymphocyte function. In this descriptive case-control prospective study, blood samples of selected HIV-infected patients on ART were collected during one of their periodic visit. Those with CD4 count of 100 to 200 cells/ul and those with ≥ 400 cells/ul blood were tested for Interleukin-6 level along with healthy controls by cytoflow and ELISA technique respectively. Results revealed IL-6 levels of 272.93±250.84 in those with CD4 count of 100 to 200 cells/ul, while 301.99±261.23 was obtained in patients with ≥ 400 cells/ul, while the apparently healthy controls have 145.98±156.36 within the standard range of 6.5 – 200 pg/ml. There was significant increase in the IL-6 levels when compared to the healthy controls. In HIV-Infected patients on ART, the higher the CD4 count, the higher the IL-6 cytokine level with the females generally having higher IL-6 cytokine values than the males.
Keywords
Evaluation, Cluster Differentiation-4+ Cells, Interleukin-6, HIV-Infected Patients, ART
Reference
| [01] | Cohen MS, Hellmann N, Levy JA, DeCock K, Lange J. The spread, treatment and prevention of HIV-1: evolution of a global pandemic. J. Clin. Invest. 2008; 118 (4): 1244-1254. |
| [02] | Global Fact Sheet: HIV/AIDS. 20th International AIDS Conference, Melbourne, Australia.“Stepping up the Pace”. [Last accessed on 30th August, 2017] available on www.aids2014.org |
| [03] | Joint United Nations Programme on HIV/AIDS (UNAIDS). World Aids Day Report 2011. [Last accessed on 31st August, 2017] available on www.unaids.org |
| [04] | Zaaqoq AM, Faisal A. Khasawneh FA, Roger D. Smalligan RD. Cardiovascular Complications of HIV-Associated Immune Dysfunction. Hindawi Publishing Corporation Cardiology Research and Practice. 2015; 1-8. |
| [05] | Koenig S, Fausi A. Immunology of human Immunodeficiency virus infection. In: Holmes KK, Mardh P, Sparling PA, Lemon SM, editors. Sexually Transmitted Diseases. New York: McGraw-Hill. 1999; 231-249. |
| [06] | Tsegaye A, Wolday D, Oho S, Petros B, Asseta T, Alebachew T, et al. Immunophenotyping of blood lymphocytes at birth, during childhood, and during adulthood in HIV-1-uninfected Ethiopians. Clinical Immunology. 2003; (109): 338-346. |
| [07] | Grund B, Baker JV, Deeks SG, Wolfson J, Wentworth D, Cozzi-Lepri A, Cohen CJ, Philips A, Lundgren JD, Neaton JD. Relevance of Interleukin-6 and D-Dimer for Serious Non-AIDS Morbidity and Death among HIV-Positive Adults on Suppressive Antiretroviral Therapy. 2016; PLoS ONE 11 (5): e0155100. |
| [08] | Bipath P, Levay P, Olorunju S, Viljoen M. A non-specifi biomarker of disease activity in HIV/AIDS patients from resource-limited environments. Afr Health Sci. 2015; 15 (2): 334-343. |
| [09] | Borges AH, O’Connor JL, Phillips AN, Rönsholt FF, Pett S, Vjecha MJ, Martyn A. French MA, Lundgren JD. Factors Associated With Plasma IL-6 Levels During HIV Infection. JID 2015; 212: 585-595. |
| [10] | Heinrich PC, Castell JV, Andus T. Interleukin-6 and the acute phase response. Biochem J. 1990; 265: 621–36. |
| [11] | Kaplanski G, Marin V, Montero-Julian F, Mantovani A, Farnarier C. IL- 6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol 2003; 24: 25–9. |
| [12] | Tanaka T, Kishimoto T. The biology and medical implications of interleukin-6. Cancer Immunol Res 2014; 2: 288–94. |
| [13] | Breen EC, Rezai AR, Nakajima K, et al. Infection with HIV is associated with elevated IL-6 levels and production. J Immunol 1990; 144: 480–484. |
| [14] | Neuhaus J, Jacobs DR Jr, Baker JV, et al. Markers of inflammation, coagulation, and renal function are elevated in adults with HIV infection. J Infect Dis 2010; 201: 1788–1795. |
| [15] | Armah KA, McGinnis K, Baker J, et al. HIV status, burden of comorbid disease, and biomarkers of inflammation, altered coagulation, and monocyte activation. Clin Infect Dis 2012; 55: 126–136. |
| [16] | Kuller LH, Tracy R, Belloso W, et al. Inflammatory and coagulation biomarkers and mortality in patients with HIV infection. PLoS Med 2008; 5: e203. |
| [17] | Duprez DA, Neuhaus J, Kuller LH, et al. Inflammation, coagulation and cardiovascular disease in HIV-infected individuals. PLoS One 2012; 7: e44454. |
| [18] | Borges ÁH, Silverberg MJ, Wentworth D, et al. Predicting risk of cancer during HIV infection: the role of inflammatory and coagulation biomarkers. AIDS 2013; 27: 1433–1441. |
| [19] | Borges AH, Weitz JI, Collins G, et al. Markers of inflammation and activation of coagulation are associated with anaemia in antiretroviral treated HIV disease. AIDS 2014; 28: 1791–1796. |
| [20] | Nordell AD, McKenna M, Borges ÁH, Duprez D, Neuhaus J, Neaton JD; INSIGHT SMART, ESPRIT Study Groups; SILCAAT Scientific Committee. Severity of cardiovascular disease outcomes among patients with HIV is related to markers of inflammation and coagulation. J Am Heart Assoc 2014; 3: e000844. |
| [21] | Okafor GO. Human Immunodeficiency Virus Infection. Ed. Salimonu LS. Basic Immunology for Students of Medicine and Biology. 3rd Edition. College Press. 2016; 175-196. |
| [22] | Srinivasula S, Lempicki RA, Adelsberger JW, Huang C, Roark J, Lee PI, Rupert A, Stevens R, Sereti I, Lane HC, Mascio MD, Kovacs JA. Differential effects of HIV viral load and CD4 count on proliferation of naive and memory CD4 and CD8 T lymphocytes. Blood. 2011; 118 (2): 262-270. |
| [23] | HIV/AIDS Surveille in Europe. European Centre for the Epidemiological Monitoring of AIDS. Med-year report 2002, no. 67. |
| [24] | Maini MK, Gilson RJ, Chavda N, Gill S, Fakoya A, Ross EJ, et al. Refrence ranges and sources of variability of CD4 counts in HIV seronegative women and men. Genitourin Med. 1996; 72: 27-31. |
| [25] | Tuomola RE, Kalish LA, Zorilla C, Fox H, Shaeref W, Landey A, et al. Changes in total CD4+ and CD8+ lymphocytes during pregnancy and 1 year Page 10 |
| [26] | Klein SL, Roberts CW. Sex hormones and immunity to infection. Editors, Berlin: Springer-Verlag. 2010 |
| [27] | Klein SL, Roberts CW. Sex and gender differences in infection and treatments for infectious diseases. Editors, Switzerland: Springer International Publishing. 2015. |
| [28] | Berghofer B, Frommer T, Haley G, Fink L, Bein G, Hackstein H. TLR7 Ligands Induce higher IFN-alpha production in females. Journal of Immunology 2006. 177; 4: 2088-2096. |
| [29] | Gleicher N, Barad DH. Gender as risk factor for autoimmune diseases. Journal of Autoimmunity. 2007; 28; 1: 1-6. |
| [30] | Rubtsov AV, Rubtsova K, Kappler JW, Marrack P. Genetic and hormonal factors in female-biased autoimmunity. Journal of Autoimmunity Review 2010. 9; 7: 494-498. |
| [31] | Reuben DB, Cheh AI, Harris TB, Ferrucci L, Rowe JW, Tracy RP, Seeman TE. Peripheral blood markers of inflammation predict mortality and functional decline in high-functioning community-dwelling older persons. Journal of the American Geriatrics Society. 2002; 50: 638–44. |
