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Vol.1 , No. 2, Publication Date: Jul. 7, 2014, Page: 57-64
 were positive for malaria while 60 (47.6%) were negative. Haematological parameters were analyzed using Mythic 22 CT 5- part differential Haematology analyzer (Orphée, Switzerland). Testing for malaria was carried out using the Onset Malaria Plasmodium falciparum (Pf) antibody (Ab) rapid test (CTK Biotech, Inc. USA) and speciation and number of parasites per high field was carried out on the Giemsa stained thing blood film. Results: The mean PCV, haemoglobin and platelet count of plasmodium- parasitized children was significantly lower compared to un-infected controls (29.48, 10.36 and 188.68) versus (32.76, 11.34 and 327.50) respectively (p=0.01). The prevalence of anaemia and thrombocytopenia was significantly higher among Plasmodium parasitized subjects compared to non-parasitized controls. Plasmodium falciparum was the predominant specie among the parasitized subjects. A negative and significant correlation was observed between the high number of parasite per high field and platelet count as index of thrombocytopenia and haemoglobin as index of anaemia (r=0.62 and p=0.75) respectively (p= 0.01) among parasitized subjects. Plasmodium parasitaemia was more prevalent among children in the 2-5 years age group (52.4%) compared to children in the 6-11 years age group (47.6%). Male children were more predisposed to malaria (53.0%) compared to female children (47.0%). Conclusion: Plasmodium parasitaemia has a significant impact on the haemoglobin, packed cell volume and platelet count of malaria parasitized children in Sokoto, Nigeria. Preventative strategies including regular chemoprophylaxis, intermittent preventative treatment with antimalarials, provision of iron supplementation and insecticide-treated bed nets should be implemented urgently to prevent the negative impact of malaria parasitaemia on the haematological parameters of children in the area. There is need for community and peer-based awareness and education initiatives to strengthen the malaria prevention programme by educating parents on the benefits of effective environmental sanitation to destroy the breeding sites of Anopheles mosquito –the vector of malaria.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Erhabor Osaro, Department of Haematology and Transfusion Science, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| [2] | Mohammad Horo Jamilu, Department of Haematology and Transfusion Science, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| [3] | Ahmed HM, Department of Haematology and Transfusion Science, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| [4] | Ezimah ACU, Department of Haematology and Transfusion Science, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria. |
Objectives: Malaria infection is a major public health problem and cause of morbidity and mortality particularly among children in tropical and subtropical regions of the world. The aim of this present study was to determine the effect of plasmodium parasitaemia on some haematological parameters of children visiting the children emergency unit of Sokoto State Specialist Hospital in Sokoto, North Western, Nigeria. Method: This study was conducted among 126 children aged 2-11 years with mean age 5.36 ± 2.50 years presenting to the children emergency unit of Sokoto Specialist Hospital with history of febrile illness. Out of the children studied, 66 (52.4%) were positive for malaria while 60 (47.6%) were negative. Haematological parameters were analyzed using Mythic 22 CT 5- part differential Haematology analyzer (Orphée, Switzerland). Testing for malaria was carried out using the Onset Malaria Plasmodium falciparum (Pf) antibody (Ab) rapid test (CTK Biotech, Inc. USA) and speciation and number of parasites per high field was carried out on the Giemsa stained thing blood film. Results: The mean PCV, haemoglobin and platelet count of plasmodium- parasitized children was significantly lower compared to un-infected controls (29.48, 10.36 and 188.68) versus (32.76, 11.34 and 327.50) respectively (p=0.01). The prevalence of anaemia and thrombocytopenia was significantly higher among Plasmodium parasitized subjects compared to non-parasitized controls. Plasmodium falciparum was the predominant specie among the parasitized subjects. A negative and significant correlation was observed between the high number of parasite per high field and platelet count as index of thrombocytopenia and haemoglobin as index of anaemia (r=0.62 and p=0.75) respectively (p= 0.01) among parasitized subjects. Plasmodium parasitaemia was more prevalent among children in the 2-5 years age group (52.4%) compared to children in the 6-11 years age group (47.6%). Male children were more predisposed to malaria (53.0%) compared to female children (47.0%). Conclusion: Plasmodium parasitaemia has a significant impact on the haemoglobin, packed cell volume and platelet count of malaria parasitized children in Sokoto, Nigeria. Preventative strategies including regular chemoprophylaxis, intermittent preventative treatment with antimalarials, provision of iron supplementation and insecticide-treated bed nets should be implemented urgently to prevent the negative impact of malaria parasitaemia on the haematological parameters of children in the area. There is need for community and peer-based awareness and education initiatives to strengthen the malaria prevention programme by educating parents on the benefits of effective environmental sanitation to destroy the breeding sites of Anopheles mosquito –the vector of malaria.
Keywords
Plasmodium Parasitaemia, Haematological Parameters, Children, Sokoto, Nigeria
Reference
| [01] | World Health Organization. World Malaria Report. Geneva: WHO; 2011. |
| [02] | Mia MS, Begum RA, Er AC, Abidin RD, Pereira JJ. Burden of malaria at household level: a baseline review in the advent of climate change. J Environ Sci Technol 2011; 5:1-15. |
| [03] | Ogbodo SO, Okeke AC, Obu HA, Shu EN, Chukwura EF. Nutritional status of parasitaemic children from malaria endemic rural communities in Eastern Nigeria. Curr Pediatr Res 2010; 14: 131-135. |
| [04] | Tchinda GG, Atashili J, Achidi EA, Kamga HL, Njunda AL, et al. Impact of Malaria on Hematological Parameters in People Living with HIV/AIDS Attending the Laquintinie Hospital in Douala, Cameroon. PLoS ONE 2012; 7(7): e40553. |
| [05] | Greenwood B, Mutabingwa T. Introduction Malaria in 2002. Nature 2002; 415: 670-672. |
| [06] | Aduragbenro D, Adedapo A, Catherine O, Falade AB, Rachel T, Kotila C, George O, Ademowo AB. Age as a risk factor thrombocytopenia and anaemia in children treated for acute uncomplicated falciparum malaria. J Vector Borne Dis 2007: 266271. |
| [07] | World Health Organization. World Health Director General’s Report. Fighting Disease, Fostering Development. Geneva: WHO; 1996. |
| [08] | UNAIDS. UNAIDS Report on the Global AIDS Epidemic 2010. UNAIDS, Geneva. |
| [09] | Ejezie GC, Ezednanchi EN, Usanga EA, Gemade EI, Ikpati NW, Alaribe AA. Malaria and its treatment in rural villages of Aboh Mbaise, Imo State, Nigeria. Acta Tropica 1991; 48: 17–24. |
| [10] | Federal Ministry of Health. National Strategic Plan for Roll Back Malaria in Nigeria 2001. Abuja: Federal Ministry of Health, Nigeria; 2001. |
| [11] | UNICEF. Partnering to Roll Back Malaria in Nigeria’s Bauchi State at a Glance. Abuja, Nigeria: Nigeria United Nations Children Fund; 2009. |
| [12] | Maina RN, Douglas Walsh, Charla Gaddy, Gordon Hongo, John Waituumbi, Lucas Otieno, Danel Jones and Bernhard R Ogutu. Impact of plasmodium falciparum infection in haematological parameters in children living in western Kenya. Malaria Journal 2010; 9 (3): 54. |
| [13] | Ovuakporaye SI Effect of malaria parasite on some haematological parameters: red blood cell count, packed cell volume and haemoglobin concentration. Journal of Medical and Applied Biosciences 2011; 3: 45-51. |
| [14] | Maina R, Walsh D, Gaddy C, Hongo G, Waitumbi J, Otieno L et al. Impact of Plasmodium falciparum infection on haematological parameters in children living in Western Kenya. Malaria J 2010; 9: S3–S4. |
| [15] | Imoru M, Shehu MA, Ihesiulor UG, Kwaru AH. Haematological changes in malaria-infected children in North-West Nigeria. Turk J Med Sci 2013; 43:838-842. |
| [16] | Blanc B, Finch CA, Hallberg L. Nutritional anaemias. Report of a WHO Scientific Group. WHO Tech Rep Ser 1968; 405: 1-40. |
| [17] | Jasbir KS, Kiersten BJ. Reduction in the burden of malaria anaemia in Benin Conformation of an anti-vector approach at National level. International journal of tropical Medicine 2009; 4 (3):104-111. |
| [18] | National Population Commission (NPC). National Census Figures, Abuja, Nigeria. 2007. |
| [19] | World Health Organisation. The global malaria situation: Current tools for prevention and control. Global Fund to fight AIDS, Tuberculosis, and Malaria. 55 World Assembly, WHO, Document 2002; No. A551. INF. Doc. 16. |
| [20] | Ejezie GC, Ezednanchi EN, Usanga EA, Gemade EI, Ikpati NW, Alaribe AA. Malaria and its treatment in rural villages of Aboh Mbaise, Imo State, Nigeria. Acta Tropica 1991; 48: 17–24. |
| [21] | Mbanugo JI, Ejim DO. Plasmodium Infections in children aged 0-5 yrs in Akwa metropolis, Anambra State, Nigeria. Nigerian Journal of Parasitology 2000; 21: 55-59. |
| [22] | Imam TS. Anaemia and Malaria in children attending two selected Paediatric clinics in Kano metropolis, Northern Nigeria. International Journal of Biomedical and Health Sciences 2009; 5(3): 133-138. |
| [23] | Olasehinde GI, Ajayi AA, Taiwo SO, Adekeye BT, Adeyeba OA. Prevalence and Management of falciparum Malaria among infant and children in Ota, Ogun State, Southwestern Nigeria. African journal of clinical and experimental Microbiology 2010; 11(3): 159-163. |
| [24] | Nwaorgu OC, Orajaka BN. Prevalence of Malaria among children 1-10 years old in communities in Akwa North Local Government Area, Anambra State South east Nigeria. International Multidisciplinary journal, Ethopia 2010; 5(5): 264-281. |
| [25] | Fowowe AA. Malaria a major cause of anaemia among under children on Hospital Bed in State Specialist Ondo, Ondo State, Nigeria. Nigerian Journal of General Practice 2011; 9(2): 26 – 50. |
| [26] | Imam T.S. and Idabawa II. Prevalence of anaemia associated with Malaria among patients in the extreme age groups attending three selected Hospital in Kano. Biol. Environ. Sci. J Tropics 2007; 4(2):137-142. |
| [27] | Abdullahi K, Abubakar U, Adamu T, Daneji AI, Aliyu RU, Jiya N, Ibrahim MTO, Nata`ala SU. Malaria in Sokoto, North western Nigeria. African Journal of Biotechnology 2009; 8 (24): 7101-7105. |
| [28] | Igbeneghu C. Anaemia and Malaria in children attending diagnostic Laboratory in Ibadan, Nigeria J Res. Bioscience 2005; 1(1):24-28. |
| [29] | Bouyou-Akotet MK, Arnaud D, Eric K, Diane E, Edgard BN, Timothy P, Jean K, Marryvonne K. Impact of Plasmodium falciparum Infections on the frequency of moderate to severe anaemia in children below 10 years of age in Gabon. Malar J 2009; 8:166. |
| [30] | Valerian LK, Wendy PO, Richard M, Fred KN, Godfrey K, Enos B, Tom L. Marion KA, Fred M, David S, Ronald HG, Kara KW. High prevalence of Malaria parasitaemia and anaemia among Hospitalized children in Rakai, Uganda 2013; DOI:10.1371/journal.pone.0082455. |
| [31] | Bakhubaira S. Haematological Parameters in Severe Complicated Plasmodium falciparum Malaria among Adults in Aden. Turk J Haematol. 2013; 30(4):394-399. |
| [32] | Olanrewaju WI, Johnson AW. Malaria in Children in Ilorin, Nigeria. East Afr Med J 2001; 78(3): 131-134. |
| [33] | Ogbodo SO, Okeke AC, Obu HA, Shu EN, Chukwura EF. Nutritional status of parasitaemic children from malaria endemic rural communities in Eastern Nigeria. Curr Pediatr Res 2010; 14: 131-135. |
| [34] | George OI. Ewelike-Ezeani CS. Haematological changes in children with malaria infection in Nigeria. L Med Sci 2011; 2: 768-771. |
| [35] | Wickramasinghe SN, Abdalla SH. Blood and bone marrow changes in malaria. Bailliere’s Clin Hematol 2000; 13: 277–299 |
| [36] | Weatherall DJ, Miller LH, Baruch DI, Marsh K, Doumbo OK, Casals-Pascual C, Roberts DJ. Malaria and the red cell. Hematology Am Soc Hematol Educ Program 2002:35–57. |
| [37] | Bashawri LA, Mandil AA, Bahnassy AA, Ahmed MA. Malaria: hematological aspects. Ann Saudi Med. 2002; 22:372–376 |
| [38] | Menendez C, Fleming AF, Alonso PL. Malaria-related anaemia. Parasitol Today 2000; 16:469–476. |
| [39] | Waitumbi JN, Opollo MO, Muga RO, Misore AO, Stoute JA. Red cell surface changes and erythrophagocytosis in children with severe Plasmodium falciparum anemia. Blood 2000; 95:1481–1486. |
| [40] | Davenport GC, Ouma C, Hittner JB, Were T, Ouma Y, Ong'echa JM, Perkins DJ. Hema-tological predictors of increased severe anemia in Kenyan children coinfected with Plasmodium falciparum and HIV-1. Am J Hematol 2010; 85(4):227-233. |
| [41] | Kumar AS. Thrombocytopenia-an indicator of acute vivax malaria. Indian J Pathol Microbiol. 2006; 49(4):505–508. |
| [42] | Adedapo AD, Falade CO, Kotila RT, Ademowo GO, George OA. Age as a risk factor for thrombocytopenia and anaemia in children treated for acute uncomplicated falciparum malaria. J Vector Borne Dis 2007; 44: 266–271 |
| [43] | Gajanand Singh Tanwar, Punam Chand Khatri, Chandra Kumar Chahar, Ghanshyam Singh Sengar, Abhishek Kochar, Gayatri Tanwar, Shaifali Chahar, Nimish Khatri, Sheetal Middha, Jyoti Acharya, Sanjay Kumar Kochar, Deepak Pakalapati, Shilpi Garg, Ashis Das, Dhanpat Kumar Kochar. Thrombocytopenia in childhood malaria with special reference to P. vivax mono-infection: A study from Bikaner (Northwestern India). Platelets 2012; 23:211-216. |
| [44] | Rogerson SJ, Hvid L, Duffy PE, Leke RFG, Taylor DW. Malaria in pregnancy: pathogenesis and immunity. Lancet Infect Dis 2007; 7: 105–117. |
| [45] | Lacerda MV, Mourão MP, Coelho HC, Santos JB. Thrombocytopenia in malaria: who cares? Mem Inst Oswaldo Cruz 2011; 106 1:52-63. |
| [46] | Abdalla S, Pasvol G. Platelets and blood coagulation in human malaria. In: Newton PN, Essien E, White NJ, editors. The Haematology of Malaria. London, UK: Imperial College Press; 2004: 249–276. |
| [47] | Tan SO, McGready R, Zwang J, et al. Thrombocytopenia in pregnant women with malaria on the Thai-Burmese border. Malar J. 2008; 7: 209. |
| [48] | Rogerson SJ, Hvid L, Duffy PE, Leke RFG, Taylor DW. Malaria in pregnancy: pathogenesis and immunity. Lancet Infect Dis. 2007; 7: 105–117 |
| [49] | Tanwar GS, Khatri PC, Chahar CK, Sengar GS, Kochar A, Tanwar G, Chahar S, Khatri N, Middha S, Acharya J, Kochar SK, Pakalapati D, Garg S, Das A, Kochar DK. Thrombocytopenia in childhood malaria with special reference to P. vivax monoinfection: A study from Bikaner (Northwestern India. Platelets. 2012; 23(3):211-216. |
| [50] | Erhabor O, Adias TC, Hart ML. Effects of falciparum malaria on the indices of anaemia among pregnant women in the Niger Delta of Nigeria. Clinical Medicine and Research 2010; 2(3): 035-041. |
| [51] | Chikwem JO, Mohammed I, Okara GC, Ukwandu NC, Ola TO. Prevalence of transmissible blood infections among blood donors at the University of Maiduguri Teaching Hospital Maiduguri, Nigeria. East Afr Med J 1997; 74: 213–216. |
| [52] | Enato EF, Mens PF, Okhamafe AO, Okpere EE, Pogoson E, Schallig HD. Plasmodium falciparum malaria in pregnancy: prevalence of peripheral parasitaemia, anaemia and malaria care-seeking behaviour among pregnant women attending two antenatal clinics in Edo State, Nigeria. J. Obstet. Gynaecol 2009; 29(4): 301-316. |
| [53] | Krafts K, Hempelmann E, Oleksyn B (2011). The color purple: from royalty to laboratory, with apologies to Malachowski. Biotech Histochem 86 (1): 7–35. |
