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Vol.2 , No. 2, Publication Date: Apr. 18, 2015, Page: 8-13
 bearing different degrees of gingival inflammation were selected to perform the present study. The periodontal inflammatory processes, as including criterion, were clinically diagnosed as mild, moderate and severe in 56%, 37% and 7% of the selected individuals, respectively. Gum biopsies were taken from the included patients and processed combining immunohistopathological and molecular (PCR) methods in order to assess the persistence of Trypanosoma cruzi-tissue forms in long-term CCP. In 24 of the 60 CCP (40%) T. cruzi persistence was confirmed. This included the presence of the parasite itself detected in 18.3% of the samples processed by Giemsa stain, and the observation of T. cruzi antigenic deposits in 28.3% and 23.3% samples when peroxidase-anti-peroxidase technique and immunofluorescence test, were used respectively. In addition, the PCR-based method revealed persistence of a portion of the T. cruzi genome in 20% of the study samples. Interestingly, 87.5% of the CCP had been treated with Benznidazole when they suffered the acute phase of the disease, which suggests an unsatisfactory effect of the drug to eliminate the T. cruzi-tissue forms. The biological significance of T. cruzi persistence in the human host is discussed and, its role on the host-parasite relationships is considered.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Néstor Añez, Center for Parasitological Research “J.F.Torrealba”, Faculty of Sciences, University of Los Andes, Merida, 5101, Venezuela. |
| [2] | Gladys Crisante, Center for Parasitological Research “J.F.Torrealba”, Faculty of Sciences, University of Los Andes, Merida, 5101, Venezuela. |
| [3] | Sonia Araujo, Laboratory of Experimental Parasitology, Department of Biology, Faculty of Sciences, University of Los Andes, Merida, 5101, Venezuela. |
| [4] | Marinest Añez, Faculty of Dentistry, University of Los Andes, Merida, Venezuela. |
| [5] | Agustina Rojas, Center for Parasitological Research “J.F.Torrealba”, Faculty of Sciences, University of Los Andes, Merida, 5101, Venezuela. |
| [6] | Henry Parada, General Hospital “Luis Razetti” Cardiological Unit, Barinas, Venezuela. |
Sixty unquestionable chronic chagasic patients (CCP) bearing different degrees of gingival inflammation were selected to perform the present study. The periodontal inflammatory processes, as including criterion, were clinically diagnosed as mild, moderate and severe in 56%, 37% and 7% of the selected individuals, respectively. Gum biopsies were taken from the included patients and processed combining immunohistopathological and molecular (PCR) methods in order to assess the persistence of Trypanosoma cruzi-tissue forms in long-term CCP. In 24 of the 60 CCP (40%) T. cruzi persistence was confirmed. This included the presence of the parasite itself detected in 18.3% of the samples processed by Giemsa stain, and the observation of T. cruzi antigenic deposits in 28.3% and 23.3% samples when peroxidase-anti-peroxidase technique and immunofluorescence test, were used respectively. In addition, the PCR-based method revealed persistence of a portion of the T. cruzi genome in 20% of the study samples. Interestingly, 87.5% of the CCP had been treated with Benznidazole when they suffered the acute phase of the disease, which suggests an unsatisfactory effect of the drug to eliminate the T. cruzi-tissue forms. The biological significance of T. cruzi persistence in the human host is discussed and, its role on the host-parasite relationships is considered.
Keywords
Trypanosoma cruzi, Chagas Disease, Parasite Persistence, Host Tissue
Reference
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