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Vol.1 , No. 4, Publication Date: Oct. 16, 2014, Page: 170-175
 Enterobacteria Isolated from Different Ecosystems in Abidjan, Ivory Coast&description=Aim: To show the prevalence of qnr genes in Enterobacteria producing a beta-lactamase resistant to nalidixic acid extended spectrum and ciprofloxacin isolated from various ecosystems such as human, animals and the environment. Materials and methods: The multiresistant Enterobacteria strains (n = 155) were isolated from a selective homemade medium (Drigalski + 2mg /l of ceftazidime) of diverse ecosystems (humans, animals and environments). Resistance to antibiotics (beta-lactams and quinolones) has been evaluated by the diffusion method and detection of beta-lactamases extended spectrum (ESBL) was carried out as recommended by NCCLS. Characterization of quinolone resistance genes (qnr A, qnr B and S qnr) was performed by multiplex PCR. Results: high levels of percent resistance to quinolones and fluoroquinolones (35% to 95%) and 35% to 93.2% respectively to nalidixic acid and ciprofloxacin were observed. The qnr gene S was detected in isolates from different ecosystems with very high prevalence strains (95%) in animals. However qnrA and qnr B genes were not detected. Conclusion: The emergence of new resistance mechanisms adopted by some strains of Enterobacteria especially in human and in the environment represents a threat to public health.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | Ouattara Mohamed Baguy, Pasteur Institute, Abidjan, Ivory Coast; Laboratory of Microbiology and Biotechnology, University of Nangui Abrogoua, Ivory Coast. |
| [2] | Guessennd Kouadio Nathalie, Pasteur Institute, Abidjan, Ivory Coast. |
| [3] | Coulibaly N’Golo David, Pasteur Institute, Abidjan, Ivory Coast. |
| [4] | Saraka N’Guessan Daniel, Pasteur Institute, Abidjan, Ivory Coast. |
| [5] | Coulibaly Kalpy Julien, Pasteur Institute, Abidjan, Ivory Coast. |
| [6] | Koffi-Nevry Rose, Laboratory of Microbiology and Biotechnology, University of Nangui Abrogoua, Ivory Coast. |
| [7] | Ouattara Gnoh Djénéba, Pasteur Institute, Abidjan, Ivory Coast. |
| [8] | Gbonon Valérie, Pasteur Institute, Abidjan, Ivory Coast. |
| [9] | Tiekoura Konan Bertin, Pasteur Institute, Abidjan, Ivory Coast. |
| [10] | Dosso Mireille, Pasteur Institute, Abidjan, Ivory Coast. |
Aim: To show the prevalence of qnr genes in Enterobacteria producing a beta-lactamase resistant to nalidixic acid extended spectrum and ciprofloxacin isolated from various ecosystems such as human, animals and the environment. Materials and methods: The multiresistant Enterobacteria strains (n = 155) were isolated from a selective homemade medium (Drigalski + 2mg /l of ceftazidime) of diverse ecosystems (humans, animals and environments). Resistance to antibiotics (beta-lactams and quinolones) has been evaluated by the diffusion method and detection of beta-lactamases extended spectrum (ESBL) was carried out as recommended by NCCLS. Characterization of quinolone resistance genes (qnr A, qnr B and S qnr) was performed by multiplex PCR. Results: high levels of percent resistance to quinolones and fluoroquinolones (35% to 95%) and 35% to 93.2% respectively to nalidixic acid and ciprofloxacin were observed. The qnr gene S was detected in isolates from different ecosystems with very high prevalence strains (95%) in animals. However qnrA and qnr B genes were not detected. Conclusion: The emergence of new resistance mechanisms adopted by some strains of Enterobacteria especially in human and in the environment represents a threat to public health.
Keywords
Ecosystems, Enterobacteria, ESBL, Quinolones Resistance Genes, Qnr
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