AASCIT - Journal - All Issues

ISSN: 2375-3811

International Journal of Biological Sciences and Applications

The Journal

Manuscript Information

Optimization of Culture Conditions for Lipase Production by Micrococcus Sp. Isolated from Farmed Clarias Gariepinus Intestine

International Journal of Biological Sciences and Applications
Vol.1 , No. 3, Publication Date: Aug. 26, 2014, Page: 79-83

1741 Views Since August 26, 2014, 848 Downloads Since Apr. 14, 2015

Paper in PDF (249K)

Follow on

Authors

[1]	Ariole C. N., Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State, Nigeria.
[2]	Bagshaw D. G., Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State, Nigeria.

Abstract

The culture conditions for maximum lipase activity by Micrococcus sp. previously isolated from Clarias gariepinus intestine were optimized. The varied culture parameters assessed were incubation time (12 hourly for 60hours), temperature (25, 30, 34, 37, 40 and 45oC), medium pH (4, 5, 6, 7, 8 and 9), additional carbon source (glucose, sucrose, lactose, maltose and mannitol), nitrogen source (peptone, urea, yeast extract, ammonium nitrate, potassium nitrate, calcium nitrate and ammonium hydrogen phosphate) and metal ions (Mn2+, Cu2+, Fe2+, Zn2+, Mg2+ and Ca2+). The lipase activity was measured using titrimetric method and olive oil as substrate. Maximum lipase activity of 27.6U/ml was found in 48h, 37oC and pH 7.0. Among all the additional carbon sources, maltose gave the maximum activity of 30.6U/ml and among all the nitrogen sources and metal ions peptone and Ca2+ gave maximum activity of 27.6U/ml and 27.7U/ml respectively. The present study has revealed Micrococcus sp. from a freshwater fish intestine as a candidate for potential industrial production of lipase.

Keywords

Lipase Activity, Micrococcus, Fish Intestine, Media Parameters, Optimization

Reference

[01]	Gilham, D. and Lehner, R. (2005). Techniques to measure lipase and esterase activity in vitro. Methods, 36:139-147.
[02]	Angkawidjaja, C. and Kanaya, S. (2006). Family 1.3 lipase: Bacterial lipases secreted by the type I secretion system. Cell. Mol. Life Sci. 63: 2804-2817.
[03]	Ghasemi, Y., Rasoul-Amini, S., Kazemi, A., Zarrini, G., Morowvat, M. H. and Kargar, M. (2011). Isolation and Characterization of Some Moderately Halophilic Bacteria with Lipase Activity. Microbiology, 80(4): 483-487.
[04]	Sharma, R., Chisti, Y. and Banerjee, U. C. (2001). Production, purification, characterization and applications of lipases. Biotechnology Advances 19: 627-662.
[05]	Gupta, R., Gupta, N. and Rathi, P. (2004). Appl. Microbiol. Biotechnol. 64: 763-781.
[06]	Cho, A., Yoo, S. and Kim, E. (2000). Cloning, Sequencing and Expression in Escherichia coli of a Thermophilic Lipase from Bacillus thermoleovorans ID-1, FEMS. Microbiol. Lett., 186(2): 235-238.
[07]	Kumar, S., Kikon, K., Upadhyay, A., Kanwar, S. and Gupta, R. (2005). Production, Purification and Characterization of Lipase from Thermophilic and Alkaliphilic Bacillus coagulans BTS-3, Protein. Expr. Purif., 41(1): 38-44.
[08]	Li, H. and Zhang, X. (2005). Characterization of Thermostable Lipase from Thermophilic Geobacillus sp. TW1, Protein. Expr. Purif. 42(1): 153-159.
[09]	Wang, Y., Srivastava, K.C., Shen, G.J. and Wang, H.Y. (1995). Thermostable alkaline lipase from a newly isolated thermophilic Bacillus strain, A30-1 (ATCC 53841). J.Ferment Bioeng. 79: 433-438.
[10]	Ariole, C.N., Nwogu, H.A. and Chuku, P.W. (2014). Enzymatic Activities of Intestinal Bacteria Isolated from Farmed Clarias gariepinus. International Journal of Aquaculture, 4(18): 108-112.
[11]	Wiseman, A. (1995). Introduction to Principles. In: Handbook of Enzyme Biotechnology, Wiseman, A. (ed.), 3rd ed. Ellis Horwood Ltd., T. J. Press Ltd., Padstow, Cornwall, UK, pp. 3-8.
[12]	Griebeler, N., Polloni, A.E., Remonatto, D., Arbter, F., Vardanega, R., Cechet, J.L. (2011). Isolation and screening of lipase producing fungi with hydrolytic activity. Food and Bioprocess Technology. 4: 578-586.
[13]	Ertuğrul, S., Dӧnmez, G. and Takac, S. (2007). Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity. J. Hazard Mater, 149(3): 720-724.
[14]	Amoozegar, M., Salehghamari, E., Khajeh, K., Kabiri, M. and Naddaf, S. (2008).
[15]	Production of an Extracellular Thermohalophilic Lipase from a Moderately Halophilic Bacterium, Salinivibrio sp. Strain SA-2, J. Basic. Microbiol., 48(3): 160-167.
[16]	Doukyu, N. and Ogino, H. (2010). Organic solvent-tolerant enzymes, Biochem. Eng. J. 48(3): 270-282.
[17]	Sahu, G.K. and Martin, M. (2011). Optimization of growth conditions for the production of extracellular lipase by bacterial strains from dairy industry effluents. Biotechnol. Bioinf. Bioeng. 1(3): 305-311.
[18]	Veerapagu, M., Narayanan, A. S., Ponmurugan, K. and Jeya, K.R. (2013). Screening selection identification production and optimization of bacterial lipase from oil spilled soil. Asian Journal of Pharmaceutical and Clinical Research. 6(3): 62- 67.
[19]	Jensen, R.G. (1983). Detection and determination of lipase (acylglycerol hydrolase) activity from various sources, Lipids, 18: 650-657.
[20]	Kumar, A., Parihar, S.S. and Batra, N. (2012). Enrichment, isolation and optimization of lipase-producing Staphylococcus sp. from oil mill waste (Oil cake). Journal of Experimental Sciences. 3(8): 26-30.
[21]	Abada, E. A.E. (2008). Production and characterization of a mesophilic lipase isolated from Bacillus stearothermophilus AB-1. Pakistan Journal of Biological Sciences. 11: 110-1106.
[22]	Šyvokienė, J., Stankus, S. and Andreikėnaitė, L. (2011). Bacterioflora of digestive tract of fishes in vitro, Veterinarija ir zootechnika, 56(78), 93-101.
[23]	Sirisha, E., Narasu, M.L. and Rajasekar, N. (2010). Isolation and optimization of lipase producing bacteria from oil contaminated soils. Advances in Biological Research. 4(5): 249-252.
[24]	Lomthaisong, K., Buranarom, A. and Niamsup, H. (2012). Investigation of isolated lipase producing bacteria from oil-contaminated soil with proteomic analysis of its proteins responsive to lipase inducer. Journal of Biological Sciences. 12: 161-167.
[25]	Kiran, G.S., Shanmughapriya, S., Jayalakshmi, J., Selvin, J., Gandhimathi, R., Sivaramakrishnan, S., Arunkumar, M., Thangavelu, T., Natarajaseenivasan, K. (2008). Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057). Bioprocess Biosyst. Eng. 31: 483-492.
[26]	Yu, H., Han, J., Li, N., Qie, X. and Jia, Y. (2009). Fermentation performance and characterization of cold-adapted lipase produced with Pseudomonas Lip35. Agricultural Sciences in China. 8(8): 956-962.
[27]	Sugihara, A., Tani, T. and Tominaga, Y. (1991). Purification and characterization of a novel thermostable lipase from Bacillus sp. J. Biochem. 109: 211-215.
[28]	Sharma, R., Soni, S.K., Vohra, R.M., Jolly, R.S. Gupta, L.K. and Gupta, J.K. (2002). Production of extracellular alkaline lipase from a Bacillus sp. RSJ1 and its application in ester hydrolysis. Ind. J. Microbiol. 42: 49-54.
[29]	Kasra-Kermanshahi, R., Mobarak-Qamsari, E. and Moosavi-nejad, Z. (2011).
[30]	Isolation and identification of a novel lipase producing bacterium, Pseudomonas aeruginosa KM110 Iran. J. Microbiol. 3(2): 92-98.
[31]	Sayari, A., Agrebi, N., Jaoua, S. and Gargouri, Y. (2001). Biochemical and molecular characterization of Staphylococcus simulans lipase, Biochimie, 83(9): 863-871.
[32]	Sagar, K., Bashir,Y., Phukan, M.M. and Konwar, B.K. (2013). Isolation of lipolytic bacteria from waste contaminated soil: A study with regard to process optimization for lipase. International Journal of Scientific & Technology Research, 2(10): 214-218.
[33]	Patkar, S. A. and Bjorkling, F. (1994). Lipase inhibitors In: Woolley, P. and Peterson, S. B. (eds.) Lipases – their structure, biochemistry and application. Cambridge University Press, Cambridge, pp. 207-224.