ISSN: 2375-3811
International Journal of Biological Sciences and Applications  
Manuscript Information
 
 
Sustainability Studies of Bacteria Derived Cellulase and Its Evaluation for Wash Performance Analysis
International Journal of Biological Sciences and Applications
Vol.5 , No. 3, Publication Date: Sep. 3, 2018, Page: 34-44
166 Views Since September 3, 2018, 52 Downloads Since Sep. 3, 2018
 
 
Authors
 
[1]    

Freny Shah, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India.

[2]    

Deepti Jain, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.

[3]    

Madhusree Mitra, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.

[4]    

Shristi Ram, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.

[5]    

Gopal Bhojani, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India; Reverse Osmosis Division, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India.

[6]    

Satyavolu Venkat Vamsi Bharadwaj, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.

[7]    

Sushma Rani Tirkey, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.

[8]    

Sandhya Mishra, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.

 
Abstract
 

Wild marine strain Bacillus licheniformis KY962963, producing thermostable alkaline carboxymethyl cellulase (exoglucanase, E.C.3.2.1.91) was acquired from the Experimental Salt Farm of CSIR-CSMCRI of Bhavnagar district. Main prospective of cellulase derived from B. licheniformis is in the laundry industry. It is a first report pertaining to the wash performance ability of the extracted cellulase both in the presence and absence of commercial detergent. The reflectance data showed that cellulase concentration of 1690 U leads to better strain removal efficiency. The purified CMCase was found to be of 38 kDa. The enzyme activity was enhanced by Mn2+ (metal ion), EDTA (chelator) and 1% Triton-X (surfactant) whereas β-Me (chelator) resulted in significant reduction of enzyme activity. Activity of cellulase in the presence of disparate agronomical wastes such as wheat bran and sugarcane bagasse are quite higher when compared to chemical substrate such as CMC. Maximum cellulase production was obtained in the presence of wheat bran i.e., 447.79 U/mg which indicated that the chemical substrate could successfully be replaced with agronomical substrate to make the process more economic as well as ecofriendly. Thermostability, alkalinity and stability in the presence of salt (NaCl), metal ions, inhibitors, surfactants, commercial detergents and organic solvent delineates the application of cellulase as the potential laundry additive.


Keywords
 

Alkali-halotolerant Cellulase, Solvent Stability, Wash Performance Analysis


Reference
 
[01]    

Pancha I, Jain D, Shrivastav A, Mishra SK, Shethia B, Mishra S, Mohandas VP, Jha B (2010) A thermoactive α-amylase from a Bacillus sp. isolated from CSMCRI salt farm. Int J Biol Macromolec 47: 288-291. https://doi.org/10.1016/j.ijbiomac.2010.04.006.

[02]    

Kumar H, Mishra A, Jha B (2013) Purification and characterization of cellulase from a marine Bacillus sp. H1666 A potential agent for single step saccharification of seaweed biomass. J Mol Catal B Enzym 93: 51-56. https://doi.org/10.1016/j.molcatb.2013.04.009.

[03]    

Kaur A, Singh A, Dua A, Mahajan R (2017) Cost-effective and concurrent production of industrially valuable xylano-pectinolytic enzymes by a bacterial isolate Bacillus pumilus AJK. Prep Biochem Biotechnol 47: 8-18. https://doi.org/10.1080/10826068.2016.1155059.

[04]    

Bhat MK, Bhat S (1997) Cellulose degrading enzymes and their potential industrial applications. Biotechnol Adv 15: 583-620. https://doi.org/10.1016/S0734-9750(97)00006-2.

[05]    

Abdel-Fattah YR, El-Helow ER, Ghanem KM, Lotfy WA (2007) Application of factorial designs for optimization of avicelase production by a thermophilic Geobacillus isolate. Res J Microbiol 2: 13-23. https://doi.org/10.3923/jm.2007.13.23.

[06]    

Mayende L, Wilhelmi BS, Pletschke BI (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost. Soil Biol Biochem 38: 2963-2966. https://doi.org/10.1016/j.soilbio.2006.03.019.

[07]    

Demharter W, Hensel R (1989) Bacillus thermocloaceae sp. nov. a new thermophilic species from sewage sludge. Syst Appl Microbiol 11: 272-276. https://doi.org/10.1016/S0723-2020(89)80025-6.

[08]    

Tai SK, Lin HPP, Kuo J, Liu JK (2004) Isolation and characterization of a cellulolytic Geobacillus thermoleovorans T4 strain from sugar refinery wastewater. Extremophiles. 8: 345-349. https://doi.org/10.1007/s00792-004-0395-2.

[09]    

Jain D, Pancha I, Mishra SK, Shrivastav A, Mishra S, (2012) Purification and characterization of haloalkalinethermoactive, solvent stable and SDS-induced protease from Bacillus sp.: a potential additive for laundry detergents. Bioresour Technol. 115: 228-236. https://doi.org/10.1016/j.biortech.2011.10.081.

[10]    

Potprommanee L, Wang XQ, Han YJ, Nyobe D (2017) Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass. PloS one. 12: e0175004. https://doi.org/10.1371/journal.pone.0175004.

[11]    

Gesheva V, Vasileva-Tonkova E (2012) Production of enzymes and antimicrobial compounds by halophilic Antarctic Nocardioides sp. grown on different carbon sources. W J Microbiol Biotechnol. 28: 2069-2076. https://doi.org/10.1007/s11274-012-1009-2.

[12]    

Shanmughapriya S, Kiran GS, Selvin J, Thomas TA (2010) Optimization, purification, and characterization of extracellular mesophilic alkaline cellulase from sponge-associated Marinobacter sp. MSI032. Appl Biochem Biotechnol. 162: 625-640. https://doi.org/10.1007/s12010-009-8747-0.

[13]    

Kasana RC, Salwan R, Dhar H, Dutt S, Gulati A (2008) A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine. Curr Microbiol 57: 503-507. https://doi.org/10.1007/s00284-008-9276-8.

[14]    

Buchanan RE, Gibbons NE (1984) Bergey’s manual of systematic bacteriology, 8th ed. Science Press, Beijing, China.

[15]    

Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 173: 697-703. https://doi.org/10.1128/jb.173.2.697-703.1991.

[16]    

Wilson K (2001) Preparation of Genomic DNA from Bacteria. Curr Protoc Mol Biol. 56: 2-4. https://doi.org/10.1002/0471142727.mb0204s56

[17]    

Miller GL (1959) Modified DNS method for reducing sugars. Anal Chem. 31: 426-428.

[18]    

Bradford MT (1976) Protein measurement with the follin phenol reagent. Biochemistry. 72: 248-254.

[19]    

Laemmli UK (1976) SDS-PAGE to evaluate extent of hydrolysis, of proteins. Nature. 227: 680-685.

[20]    

Li X, Yu HY (2012) Purification and characterization of an organic-solvent-tolerant cellulase from a halotolerant isolate, Bacillus sp. L1. J Ind Microbiol Biotechnol. 39: 1117-1124. https://doi.org/10.1007/s10295-012-1120-2.

[21]    

Ko CH, Tsai CH, Lin PH, Chang KC (2010) Characterization and pulp refining activity of a Paenibacillus campinasensis cellulase expressed in Escherichia coli. Bioresour Technol. 101: 7882-7888. https://doi.org/10.1016/j.biortech.2010.05.043

[22]    

Kim CH (1995) Characterization and substrate specificity of an endo-beta-1, 4-D-glucanase I (Avicelase I) from an extracellular multienzyme complex of Bacillus circulans. Appl Environ Microbiol. 61: 959-965.

[23]    

Endo K, Hakamada Y, Takizawa S, Kubota H (2001) A novel alkaline endoglucanase from an alkaliphilic Bacillus isolate: enzymatic properties and nucleotide and deduced amino acid sequences. Appl Microbiol Biotechnol 57: 109-116. https://doi.org/10.1007/s002530100744.

[24]    

Politz O, Krah M, Thomsen KK, Borriss R (2000) A highly thermostable endo-(1, 4)-β-mannanase from the marine bacterium Rhodothermus marinus. Appl Microbiol Biotechnol. 53: 715-721. https://doi.org/10.1007/s002530000351.

[25]    

Ng IS, Li CW, Yeh YF, Chen PT (2009) A novel endo-glucanase from the thermophilic bacterium Geobacillus sp. 70PC53 with high activity and stability over a broad range of temperatures. Extremophiles. 13: 425-435. https://doi.org/10.1007/s00792-009-0228-4.

[26]    

Karnchanatat A, Petsom A, Sangvanich A, Piapukiew J (2008) A novel thermostable endoglucanase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.: Fr.) Rehm. Enzyme Microb Technol. 42: 404-413. https://doi.org/10.1016/j.enzmictec.2007.11.009.

[27]    

Ito S, Shdcata S, Ozaki K, Kawai S (1989) Alkaline cellulase for laundry detergents: production by Bacillus sp. KSM-635 and enzymatic properties. Agric Biol Chem 53: 1275-81. https://doi.org/10.1271/bbb1961.53.1275.

[28]    

Deka D, Jawed M, Goyal A (2013) Purification and characterization of an alkaline cellulase produced by Bacillus subtilis (AS3). Prep Biochem Biotechnol. 43: 256-270. https://doi.org/10.1080/10826068.2012.719849.

[29]    

Idris ASO, Pandey A, Rao SS, Sukumaran RK (2017) Cellulase production through solid-state tray fermentation, and its use for bioethanol from sorghum stover. Bioresour Technol. 242: 265-271. https://doi.org/10.1016/j.biortech.2017.03.092.

[30]    

Sanchez-Torres J, Perez P, Santamaría RI (1996) A cellulase gene from a new alkalophilic Bacillus sp. (strain N186-1). Its cloning, nucleotide sequence and expression in Escherichia coli. Appl Microbiol Biotechnol. 46: 149-155. https://doi.org/10.1007/s002530050797.

[31]    

Pottkämper J, Barthen P, Ilmberger N, Schwaneberg U (2009) Applying metagenomics for the identification of bacterial cellulases that are stable in ionic liquids. Green Chem. 11: 957-965. https://doi.org/10.1039/B820157A.

[32]    

Azadian F, Badoei-dalfard A, Namaki-Shoushtari A, Karami Z (2017) Production and characterization of an acido-thermophilic, organic solvent stable cellulase from Bacillus sonorensis HSC7 by conversion of lignocellulosic wastes. J Genet Eng Biotechnol. 15: 187-196. https://doi.org/10.1016/j.jgeb.2016.12.005.

[33]    

Sadhu S, Saha P, Sen SK, Mayilraj S (2013) Production, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung. Springer Plus. 2: 10. https://doi.org/10.1186/2193-1801-2-10.





 
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