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Vol.5 , No. 3, Publication Date: Sep. 3, 2018, Page: 34-44
 was acquired from the Experimental Salt Farm of CSIR-CSMCRI of Bhavnagar district. Main prospective of cellulase derived from <i>B. licheniformis</i> is in the laundry industry. It is a first report pertaining to the wash performance ability of the extracted cellulase both in the presence and absence of commercial detergent. The reflectance data showed that cellulase concentration of 1690 U leads to better strain removal efficiency. The purified CMCase was found to be of 38 kDa. The enzyme activity was enhanced by Mn<sup>2+</sup> (metal ion), EDTA (chelator) and 1% Triton-X (surfactant) whereas β-Me (chelator) resulted in significant reduction of enzyme activity. Activity of cellulase in the presence of disparate agronomical wastes such as wheat bran and sugarcane bagasse are quite higher when compared to chemical substrate such as CMC. Maximum cellulase production was obtained in the presence of wheat bran i.e., 447.79 U/mg which indicated that the chemical substrate could successfully be replaced with agronomical substrate to make the process more economic as well as ecofriendly. Thermostability, alkalinity and stability in the presence of salt (NaCl), metal ions, inhibitors, surfactants, commercial detergents and organic solvent delineates the application of cellulase as the potential laundry additive.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | Freny Shah, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India. |
| [2] | Deepti Jain, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. |
| [3] | Madhusree Mitra, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. |
| [4] | Shristi Ram, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. |
| [5] | Gopal Bhojani, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India; Reverse Osmosis Division, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India. |
| [6] | Satyavolu Venkat Vamsi Bharadwaj, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. |
| [7] | Sushma Rani Tirkey, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. |
| [8] | Sandhya Mishra, Division of Biotechnology and Phycology, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), Bhavnagar, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. |
Wild marine strain Bacillus licheniformis KY962963, producing thermostable alkaline carboxymethyl cellulase (exoglucanase, E.C.3.2.1.91) was acquired from the Experimental Salt Farm of CSIR-CSMCRI of Bhavnagar district. Main prospective of cellulase derived from B. licheniformis is in the laundry industry. It is a first report pertaining to the wash performance ability of the extracted cellulase both in the presence and absence of commercial detergent. The reflectance data showed that cellulase concentration of 1690 U leads to better strain removal efficiency. The purified CMCase was found to be of 38 kDa. The enzyme activity was enhanced by Mn2+ (metal ion), EDTA (chelator) and 1% Triton-X (surfactant) whereas β-Me (chelator) resulted in significant reduction of enzyme activity. Activity of cellulase in the presence of disparate agronomical wastes such as wheat bran and sugarcane bagasse are quite higher when compared to chemical substrate such as CMC. Maximum cellulase production was obtained in the presence of wheat bran i.e., 447.79 U/mg which indicated that the chemical substrate could successfully be replaced with agronomical substrate to make the process more economic as well as ecofriendly. Thermostability, alkalinity and stability in the presence of salt (NaCl), metal ions, inhibitors, surfactants, commercial detergents and organic solvent delineates the application of cellulase as the potential laundry additive.
Keywords
Alkali-halotolerant Cellulase, Solvent Stability, Wash Performance Analysis
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