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Vol.1 , No. 3, Publication Date: Aug. 26, 2014, Page: 62-71
 in Egyptian Governorates&description=The sweet potato whitefly Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) is one of the most damaging pests world-wide. The neonicotinoid insecticides, imidacloprid, acetamiprid, thiamethoxam, dinutefuram and the insect growth-regulating insecticides (IGRs), buprofezin and pyriproxyfen were tested against whitefly adults collected from seven Egyptian governorates. Buprofezin is recommended over pyriproxyfen due to its low LC50 values in all the tested strains. Acetamiprid showed the higher potency to whiteflies in Behira, Gharbeia, Dakahleia, and Menia governorates due to its low LC50 values in comparison with the other neonicotinoids. To detect insect response to insecticide treatment, the mixed function oxidase activity was measured in laboratory and field collected samples. CYP6 gene was detected in all of the collected samples. A fragment of (852 bp) was obtained using CYP6 degenerate primer. PCR product yielded partial P450 sequence with 99% identity to B. tabaci CYP6CX1v1. Nucleotide variations were detected among the collected samples which may be one of the reasons for the insensitivity of Cytochrome P450 to neonicotinoids application in some governorates.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | Sayeda F. Farghaley, Central Agricultural Pesticide Laboratory, Plant Protection Institute, Agricultural Research Centre, Giza, Egypt. |
| [2] | Heba M. Hamama, Entomology Department, Faculty of Science, Cairo University, Giza, Egypt. |
| [3] | Azza E. Dawood, Central Agricultural Pesticide Laboratory, Plant Protection Institute, Agricultural Research Centre, Giza, Egypt. |
The sweet potato whitefly Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) is one of the most damaging pests world-wide. The neonicotinoid insecticides, imidacloprid, acetamiprid, thiamethoxam, dinutefuram and the insect growth-regulating insecticides (IGRs), buprofezin and pyriproxyfen were tested against whitefly adults collected from seven Egyptian governorates. Buprofezin is recommended over pyriproxyfen due to its low LC50 values in all the tested strains. Acetamiprid showed the higher potency to whiteflies in Behira, Gharbeia, Dakahleia, and Menia governorates due to its low LC50 values in comparison with the other neonicotinoids. To detect insect response to insecticide treatment, the mixed function oxidase activity was measured in laboratory and field collected samples. CYP6 gene was detected in all of the collected samples. A fragment of (852 bp) was obtained using CYP6 degenerate primer. PCR product yielded partial P450 sequence with 99% identity to B. tabaci CYP6CX1v1. Nucleotide variations were detected among the collected samples which may be one of the reasons for the insensitivity of Cytochrome P450 to neonicotinoids application in some governorates.
Keywords
Bemisia Tabaci, Neonicotinoids, Resistance, Insect Growth Regulators, Mixed Function Oxidase, Cytochrome P450
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