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Vol.1 , No. 3, Publication Date: Aug. 15, 2014, Page: 55-61
 Pluripotency Enhanced by Transforming Growth Factor (TGF-β1) in Vitro may be Inhibited by Differentiation-Inducing Factors Laminin-5 and Dexamethasone&description=Mesenchymal stem cells are a derived from a variety of human tissues and are being bioengineered and studied for possible uses in the advancement of medicine. Recent efforts are being focused on Dental Pulp Stem Cells (DPSC’s) due to the accessibility of this tissue. The objective of this study was to evaluate the potential to induce differentiation of DPSC isolates in vitro by the adding of exogenous growth factors (GF), and by the coating of specific extracellular matrix molecules (ECM) onto the surface of tissue-culture dishes. Photomicroscopy and mRNA analysis demonstrated the addition of TGF-β1 noteably increased pluripotency biomarkers in DPSC lines. The addition of Dexamethasone (Dex) or plating on Laminin-5 (LN5) was correlated with changes to cellular morphology and cell size in different subsets of cells. RNA isolated from these DPSCs for relative endpoint (RE) reverse transcription polymerase chain reaction (RT-PCR) revealed mRNA DPSC specific intracellular biomarkers (Klf4, Sox-2, Bin-1, Rnf12, Oct-4 and NANOG) and the cell surface marker (CD133) were enhanced following the administration of TGF-β1 and were differentially down-regulated following Dexamethasone and Laminin-5 administration. This study provides some initial evidence that randomly selected DPSC isolates may be induced by established protocols to change phenotype and expression of pluripotent biomarkers with variable susceptibility between differing types of DPSCs. More studies will be needed to determine the range of cell types that can be successfully re-engineered in laboratory settings.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | Kelcey Loveland, Department of Clinical Sciences, University of Nevada, Las Vegas – School of Dental Medicine. |
| [2] | Aubrey Young, Department of Orthodontic and Dentofacial Orthopedics, University of Nevada, Las Vegas – School of Dental Medicine. |
| [3] | Mehrnaz Khadiv, Department of Clinical Sciences, University of Nevada, Las Vegas – School of Dental Medicine. |
| [4] | Marah Culpepper, Department of Clinical Sciences, University of Nevada, Las Vegas – School of Dental Medicine. |
| [5] | Karl Kingsley, Department of Biomedical Sciences, University of Nevada, Las Vegas – School of Dental Medicine. |
Mesenchymal stem cells are a derived from a variety of human tissues and are being bioengineered and studied for possible uses in the advancement of medicine. Recent efforts are being focused on Dental Pulp Stem Cells (DPSC’s) due to the accessibility of this tissue. The objective of this study was to evaluate the potential to induce differentiation of DPSC isolates in vitro by the adding of exogenous growth factors (GF), and by the coating of specific extracellular matrix molecules (ECM) onto the surface of tissue-culture dishes. Photomicroscopy and mRNA analysis demonstrated the addition of TGF-β1 noteably increased pluripotency biomarkers in DPSC lines. The addition of Dexamethasone (Dex) or plating on Laminin-5 (LN5) was correlated with changes to cellular morphology and cell size in different subsets of cells. RNA isolated from these DPSCs for relative endpoint (RE) reverse transcription polymerase chain reaction (RT-PCR) revealed mRNA DPSC specific intracellular biomarkers (Klf4, Sox-2, Bin-1, Rnf12, Oct-4 and NANOG) and the cell surface marker (CD133) were enhanced following the administration of TGF-β1 and were differentially down-regulated following Dexamethasone and Laminin-5 administration. This study provides some initial evidence that randomly selected DPSC isolates may be induced by established protocols to change phenotype and expression of pluripotent biomarkers with variable susceptibility between differing types of DPSCs. More studies will be needed to determine the range of cell types that can be successfully re-engineered in laboratory settings.
Keywords
Dental Pulp, Stem Cells
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