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Vol.1 , No. 3, Publication Date: Jul. 18, 2014, Page: 46-54
. strain CDBT 2 showed good ethanol tolerance with maximum growth even at 8% ethanol. Thebest ethanol tolerant strain CDBT 2 found to grow till 18% ethanol. Total ethanol production by CDBT 2 was found to enhance by about 15±0.12% than control without supply of 4V external energy when glucose was used as carbohydrate source. Drastic increase in ethanol production by 129±0.877% was seen when external voltage was supplied in electrochemical cell with alginate immobilized yeast cells on porous graphite cathode with or withput external voltage supply. However total enhancement is 7.0±0.056% than control w/o immobilization of yeast strain in cathode. 9.0±0.225% increament was observed when cathode was immobilized with neutral red with and without supply of external voltage. 30.64±0.30% and 28.67±0.344% increament in ethanol were observed when cathode were immobilized with neutral red only and neutral red followed by yeast immobilization than with normal graphite felt cathode without external voltage supply. The best ethanol tolerant yeast strain, CDBT 2 was grown in electrolytic cell at best electrode combination with lignocellulosic biomass, Saccharum spontanum, pretreated with ammonium hydroxide followed by hydrolysis with hydrochloric acid gave the ethanol production of 16.67% greater than without external voltage supply.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | Jarina Joshi, Central Department of Biotechnology, Tribhuvan University, Nepal. |
| [2] | Rejeena Shrestha, Central Department of Biotechnology, Tribhuvan University, Nepal. |
| [3] | Rojlina Manandhar, Central Department of Biotechnology, Tribhuvan University, Nepal. |
| [4] | Krishna Manandhar, Central Department of Biotechnology, Tribhuvan University, Nepal. |
| [5] | Lakshmaiah Sreerama, Department of Chemistry and Physics, St. Cloud State University, USA. |
| [6] | Tribikram Bhattarai, Central Department of Biotechnology, Tribhuvan University, Nepal. |
Electrolytic cell are used for enhancement in production of microbial products. Ethanol is produced as one of the renewable source of energy for which one can use even waste products like lignocellulosic biomass. Selection of best ethanol producing yeast strain and best ethanol tolerant yeast cell is first priority for optimized production. Out of eight yeast strains isolated in Central Department of Biotechnology (CDBT). strain CDBT 2 showed good ethanol tolerance with maximum growth even at 8% ethanol. Thebest ethanol tolerant strain CDBT 2 found to grow till 18% ethanol. Total ethanol production by CDBT 2 was found to enhance by about 15±0.12% than control without supply of 4V external energy when glucose was used as carbohydrate source. Drastic increase in ethanol production by 129±0.877% was seen when external voltage was supplied in electrochemical cell with alginate immobilized yeast cells on porous graphite cathode with or withput external voltage supply. However total enhancement is 7.0±0.056% than control w/o immobilization of yeast strain in cathode. 9.0±0.225% increament was observed when cathode was immobilized with neutral red with and without supply of external voltage. 30.64±0.30% and 28.67±0.344% increament in ethanol were observed when cathode were immobilized with neutral red only and neutral red followed by yeast immobilization than with normal graphite felt cathode without external voltage supply. The best ethanol tolerant yeast strain, CDBT 2 was grown in electrolytic cell at best electrode combination with lignocellulosic biomass, Saccharum spontanum, pretreated with ammonium hydroxide followed by hydrolysis with hydrochloric acid gave the ethanol production of 16.67% greater than without external voltage supply.
Keywords
Electrochemical Cell, Ethanol, Lignocellulosic Biomass, Voltage
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