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International Journal of Biological Sciences and Applications

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Emergence of Klebsiella pneumoniae Carrying OXA-48 Carbapenemase in Bulgaria

International Journal of Biological Sciences and Applications
Vol.3 , No. 3, Publication Date: Aug. 27, 2016, Page: 38-41

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Authors

[1]	Ivo Sirakov, Department of Medical Microbiology, Faculty of Medicine, Medical University of Sofia, Bulgaria.
[2]	Yuliya Marteva-Proevska, Central Laboratory of Microbiology, Aleksandrovska University Hospital, Sofia, Bulgaria.
[3]	Tanya Strateva, Department of Medical Microbiology, Faculty of Medicine, Medical University of Sofia, Bulgaria.
[4]	Borelli Zlatkov, Clinic of Nephrology and Transplantation, Aleksandrovska University Hospital, Sofia, Bulgaria.
[5]	Boyka Markova, Central Laboratory of Microbiology, Aleksandrovska University Hospital, Sofia, Bulgaria.
[6]	Ivan Mitov, Department of Medical Microbiology, Faculty of Medicine, Medical University of Sofia, Bulgaria.

Abstract

In the beginning of June 2013 we identified a carbapenem-nonsusceptible Klebsiella pneumoniae isolate (OXA48BG), according to the Clinical and Laboratory Standards Institute 2013 recommendations. The strain was isolated from the urine sample of female patient in a University hospital in Sofia, Bulgaria. Antimicrobial susceptibilities were determined by the Etest (LIOFILCHEM). Modified Hodge test, Carba NP test and phenotypic tests for carbapenemase detection by using the KPC, MBL and OXA-48 Confirm Kit (Rosco Diagnostica) were performed. PCR amplification and DNA sequencing of carbapenemase genes (bla_OXA-48) were carried out. OXA48BG was resistant to ampicillin, amoxicillin/clavulanic acid, piperacillin, piperacillin/tazobactam, cephalothin, cefuroxime, cefotaxime, trimethoprim/sulfamethoxazole; susceptible to ceftazidime, cefepime, aminoglycosides, fluoroquinolones and colistin; and had borderline susceptibility to imipenem and meropenem. Using phenotypic tests and molecular-genetic techniques OXA48BG was identified as OXA-48 producer. So far there are only single reports for detection of K. pneumoniae strains carrying genes for OXA-48 carbapenemase in Bulgaria.

Keywords

Klebsiella pneumonia, Class D Carbapenemases, OXA-48

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