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Vol.3 , No. 1, Publication Date: Feb. 24, 2016, Page: 15-21
-induced changes in the levels of antioxidant enzymes of rabbits were evaluated in this study. The organs examined were the blood, brain and liver. Exposure of rabbits to DNPH (28 mg/kg body weight) caused significant (P<0.05) increase in catalase and superoxide dismutase activities relative to the DNPH-free group. The activity of glucose-6-phosphate dehydrogenase was also significantly (p<0.05) elevated in the serum while the level of reduced glutathione (GSH) was significantly reduced following DNPH treatment when compared to control. However, pre-treatment with (100 mg/kg body weight) whole aqueous extract of <i>H. sabdariffa</i> (HS) and calyx anthocyanins separately provided varying degrees of protection against DNPH-induced biochemical changes. Relative to the controls, the extracts treatments significantly (P<0.05) decreased the activities of the antioxidant enzymes. Examined separately and compared, both extracts appeared to have offered effective protection against DNPH-induced oxidative damage, though the anthocyanin isolate appeared to be more effective in this capacity. So <i>H. sabdariffa</i> calyx possesses potent antioxidant principles which are likely to be anthocyanins.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | A. O. Olusola, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria. |
The effects of aqueous extract and anthocyanin-rich extract of the calyces of Hibiscus sabdariffa Linn on the 2,4-dinitrophenylhydrazine (DNPH)-induced changes in the levels of antioxidant enzymes of rabbits were evaluated in this study. The organs examined were the blood, brain and liver. Exposure of rabbits to DNPH (28 mg/kg body weight) caused significant (P<0.05) increase in catalase and superoxide dismutase activities relative to the DNPH-free group. The activity of glucose-6-phosphate dehydrogenase was also significantly (p<0.05) elevated in the serum while the level of reduced glutathione (GSH) was significantly reduced following DNPH treatment when compared to control. However, pre-treatment with (100 mg/kg body weight) whole aqueous extract of H. sabdariffa (HS) and calyx anthocyanins separately provided varying degrees of protection against DNPH-induced biochemical changes. Relative to the controls, the extracts treatments significantly (P<0.05) decreased the activities of the antioxidant enzymes. Examined separately and compared, both extracts appeared to have offered effective protection against DNPH-induced oxidative damage, though the anthocyanin isolate appeared to be more effective in this capacity. So H. sabdariffa calyx possesses potent antioxidant principles which are likely to be anthocyanins.
Keywords
Hibiscus sabdariffa, Aqueous Extract, Anthocyanin-Rich Extract, Antioxidant Enzymes, 2,4-Dinitrophenylhydrazine
Reference
| [01] | Akindahunsi, A. A. and Olaleye, M. T. (2003). Toxicological investigation of aqueous methanolic extracts of the calyces of H. sabdariffa L. J Ethnopharmacol, 89: 161-164. |
| [02] | Aoun, M. and Makris, D. P. (2012). Binary mixtures of natural polyphenol antioxidants with ascorbic acid: impact of interactions on the antiradical activity. International Food Research Journal. 19 (2) 603-606 |
| [03] | Bergmeyer, H. U., Bernt, E., Schmidt, F. and Stork, H. (1974) D-Glucose: determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Methods of enzymatic analysis 2nd English ed. Bergmeyer, H. U. (Ed) Harcourt Brace New York, NY. 1974: 1196-1201. |
| [04] | Block G (1992). The data support a role for antioxidants in reducing cancer risk. Nutrition Reviews 50: 207-213. |
| [05] | Calviello, G., Di Nicuolo, F., Serini, S., Piccioni, E., Boninsegna, A., Maggiano, N., Ranelletti, F. O. and Palozza, P. (2005). Docosahexaenoic acid enhances the susceptibility of human colorectal cancer cells to 5-fluorouracil. Cancer Chemother Pharmacol, 55 (1): 12-20. |
| [06] | Chiu, S. Y. C., Dobberstein, R. H., Hong, H. H. S. and Farnsworth, N. R. (1982). Oxoaporphine alkaloids from Siparuna gilgiana. J Nat Prod, 45: 229-230. |
| [07] | Clemens MR, Reinmer H, Waller HD (1984). Phenylhydrazine-induced lipid peroxidation of red blood cells in vitro and in vivo: Monitoring by the production of volatile hydrocarbons. Biochem. Pharmacol. 53 (11): 1715-1718. |
| [08] | Das, S. K. and Nair, R. C. (1980). Superoxide dismutase, glutathione peroxidase, catalase and lipid peroxidation of normal and sickled erythrocytes. Br J Hematol, 44 (1). 87-92 |
| [09] | Duke, J. A. and Atchley, A. A. (1984). Proximate analysis. In: Christie, B.R. (ed.) The handbook of plant science in agriculture. CRC press, Inc. Boca Raton. |
| [10] | Flynn, T. G., de Bold, M. L. and de Bold, A. J. (1983). The amino acid sequence of an atrial peptide with potent diuretic and natriuretic properties. Biochem Biophys Res Com, 117: 859-863. |
| [11] | Hertog MGL, Feskens EJM (1993). Dietary antioxidants flavonoids and risk of coronary heart disease: The Zutphen Elderly Study. Lancet 342: 1007-1011. |
| [12] | Hirunpanich, V., Utaipat, A., Morales, N. P., Bunyapraphatsara, N., Sato, H., Herunsale, A., Suthisisang, C. (2006). Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats. Journal of Ethnopharmacology 103, 252–260. |
| [13] | Hong V, Wrolstad RE (1990). Use of HPLC separation/photodiode detection for characterization of anthocyanins. J. Agric. Food Chem. 38: 708-715. |
| [14] | Jain S, Hochstein P (1979). Generation of superoxide radicals by hydrazine: Its role in phenylhydrazine-induced hemolytic anemia. Biochemica et Biophysica Acta 586: 128-136. |
| [15] | Jollow, D. J., Mitchel, J. R., Zampaghonic, A. and Gillette, J. R. (1974). Bromobenzene-induced live necrosis; protective role of glutathione and evidence for 3, 4-bromobenzeneoxide as the hepatotoxic metabolite. Pharmacology 11: 151-169. |
| [16] | Jollow, D. J., Mitchell, J. R., Zampaghonic and Gillette, J. R. (1974). Bromobenzene – induced live necrosis; protective role of glutathione and evidence for 3, 4-bromobenzeneoxide as the hepatotoxic metabolite. Pharmacology, 11: 151 – 169. |
| [17] | Kanner, J., Frankel, E., Granit, R., German, B. and Kinsella, J. (1994). Natural antioxidants in grapes and wines. J. Agric Food Chem. 42: 64-69. |
| [18] | Lagenhoven, P., Smith, M., Letchame, W. & Simon, J. 2001 Hibiscus agrobusiness in sustainable national Africa plant products (ASNAPP). HIB-FS. |
| [19] | Maduka HCC, Okoye ZSC, Eje A (2003). The influence of Sacoglottis gabonensis stem bark extract and its isolate bergenin, Nigerian alcoholic additive, on the metabolic and hematological side effects of 2,4-dinitrophenylhydrazine-induced tissue damage. Vascular Pharmacology 39: 317-324. |
| [20] | Mazza, G., Kay, C. D., Cottrell, T. and Holub, B. J. (2002). Absorption of anthocyanins from blueberries and serum antioxidant status in human subjects. J Agric Food Chem, 50 (26): 7731-7737. |
| [21] | Misra HP, Fridovich I (1972). The role of superoxide anion in the auto oxidation of epinephrine and a simple assay of superoxide dismutase. J. Biol. Chem. 2417: 3170. |
| [22] | Morton JF (1987). Rosella. In: Fruits of Warm Climates. Darling, C. F. (Ed). Media Inc. Green-shore PP 281-286. |
| [23] | Olatunji, L. A., Adebayo, J. O., Adesokan, A. A., Olatunji, V. A. and Soladoye, A. O. (2006). Chronic administration of aqueous extract of Hibiscus sabdariffa enhances Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of rat heart. Pharmaceutical Biology. 44 (3): 213-216. |
| [24] | Ologundudu A, Obi FO (2005). Prevention of 2,4-dinitrophenylhydrazine-induced tissue damage in rabbits by orally administered decoction of dried flower of Hibiscus sabdariffa L J. Med. Sci. 5 (3): 208-211. |
| [25] | Ologundudu, A., Ologundudu, A. O., Ololade, I. A. and Obi, F. O. (2009a). Effect of Hibiscus sabdariffa anthocyanins on 2,4-dinitrophenylhydrazine-induced hematotoxicity in rabbits. Afr J Biochem Res 3 (4): 140-144. |
| [26] | Ologundudu, A., Ologundudu, A. O., Ololade, I. A. and Obi, F. O. (2009b). The effect of Hibiscus anthocyanins on 2,4 dinitrophenylhydrazine-induced hepatotoxicity in rabbits. Int. J Phys Sci 4 (4): 233-237 |
| [27] | Ologundudu, A., Ologundudu, A. O., Oluba, O. M., Omotuyi, I. O. and Obi, F. O. (2010a). Effect of Hibiscus sabdariffa anthocyanins on 2,4-dinitrophenylhydrazine-induced tissue damage in rabbits. J Toxicol Envir Health Sci 2 (1): 1-6 |
| [28] | Olusola, A. O. (2014). Effects of Hibiscus sabdariffa calyx anthocyanins and ascorbate on 2,4-dinitrophenylhydrazine-induced changes in the activities of antioxidant enzymes in rabbits. Research and Reviews Journal of Pharmacology and Toxicological Studies. 2 (4): 24-30 |
| [29] | Olusola, A. O., Olusola, A. O., Bada, S. O. and Obi, F. O. (2012). Comparative study on the effect of Hibiscus sabdariffa calyx anthocyanins and ascorbate on 2,4-dinitrophenylhydrazine-induced damage in rabbits. American Journal of Biochemistry. 2 (2): 1-6. |
| [30] | Olusola A. O., Olusola, A. O., Bada, S. O. and Obi, F. O. (2012). Effects of Hibiscus sabdariffa extracts on 2,4-dinitrophenylhydrazine-induced cytotoxicity in rabbits. Frontiers in Science. 2 (6): 221-225. |
| [31] | Orringer, E. P. and Parker, J. C. (1973). Ion and water movements in red blood cells. Prog Hematol 8: 1-23 |
| [32] | Pietta, P. G. (2000). Flavonoids as antioxidants. J. Nat. Prod. 63 (7): 1035-1042. |
| [33] | SAS Institute Inc. (1999). SAS/STAT User’s Guide. Version 8 for Windows. SAS Institute Inc., SAS Campus Drive, Cary, North Carolina, USA. |
| [34] | Sinha, K. A. (1971). Colorimetric assay of catalase. Anal Biochem, 47: 389-394 |
| [35] | Snell, T. W., Fields, A. M. and Johnson, R. K. (2012). Antioxidants can extend lifespan of Brachionus manjavacas (Rotifera), but only in a few combinations. Biogerontology 13 (3): 261-275 |
| [36] | Stadtman, T. C. (1980). Selenium dependent enzymes. Ann Rev Biochem, 49, 93–110. |
| [37] | Stanner SA, Hughes J, Kelly CN, Butriss J (2004). A review of the epidemiological evidence for the antioxidant hypothesis. Pub. Health Nutr. 7 (3): 407-422. |
| [38] | Vertuani S, Augusti A, Maufredina S (2004). The antioxidants and prooxidants network: An overview. Cur. Pharm. Des. 10 (14): 1677-1694. |
| [39] | Wang CJ, Wang JM, Lin WL, Chu CY, Chou FP, Tseng TH (2000). Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food and Chemical Toxicology 38: 411-416. |
| [40] | Winterbourn, C. C., McGrath, B. M. and Carrell, R. N. (1976). Superoxide and normal and unstable hemoglobin. Biochem J, 155: 493-502. |
| [41] | Youdim, K. A., McDonald, J., Kalt, W. and Joseph, J. A. (2002). Potential role of dietary flavonoids in reducing microvascular endothelium vulnerability to oxidative and inflammatory insults. J Nutr Biochem, 13: 282-288. |
