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Vol.1 , No. 1, Publication Date: Jul. 7, 2014, Page: 19-23
; diluted control (C2); and treatments T1, T2, T3 and T4 using diluted semen with inclusion of 2, 4, 6 and 8 ml of tomato juice per 100 ml of extender, respectively. Mass activity, individual motility, dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities percentages were determined twice a week for 8 wks period after in vitro storage time of 0, 12, 24 and 36 hr of chickens’ semen in refrigerator. Results showed that adding tomato juice up to 8 ml per 100 ml of semen extender significantly (P<0.05) increased mass activity and individual motility percentages and decreased the percentages of dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities at all storage times when compared with control. No significant changes were observed in all parameters and storage times when tomato juice was added to the extender up to 6 ml per 100 ml of extender (T1, T2 and T3). Increasing the addition level of tomato juice from 6 to 8 ml per 100 ml to the extender (T4) significantly led to additional improvement in all parameters studied in vitro storage times. Improvement sperm characteristics during storage of chickens’ semen due to tomato juice addition to the extender support published concepts about the positive effects of tomato juice as an antioxidant-rich product.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | H. J. Al-Daraji , Department of Animal Production, College of Agriculture, University of Baghdad, Baghdad, Iraq. |
The aim of this experiment was to determine the effects of tomato juice inclusion in semen extender on sperm characteristics during in vitro storage of chickens’ semen. Sixty white Leghorn roosters were used in this study. They were divided into six treatments; undiluted control, fresh semen (C1); diluted control (C2); and treatments T1, T2, T3 and T4 using diluted semen with inclusion of 2, 4, 6 and 8 ml of tomato juice per 100 ml of extender, respectively. Mass activity, individual motility, dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities percentages were determined twice a week for 8 wks period after in vitro storage time of 0, 12, 24 and 36 hr of chickens’ semen in refrigerator. Results showed that adding tomato juice up to 8 ml per 100 ml of semen extender significantly (P<0.05) increased mass activity and individual motility percentages and decreased the percentages of dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities at all storage times when compared with control. No significant changes were observed in all parameters and storage times when tomato juice was added to the extender up to 6 ml per 100 ml of extender (T1, T2 and T3). Increasing the addition level of tomato juice from 6 to 8 ml per 100 ml to the extender (T4) significantly led to additional improvement in all parameters studied in vitro storage times. Improvement sperm characteristics during storage of chickens’ semen due to tomato juice addition to the extender support published concepts about the positive effects of tomato juice as an antioxidant-rich product.
Keywords
Tomato Juice, Extender, Semen Quality, Liquid Storage, Chicken Semen
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