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Vol.1 , No. 4, Publication Date: Oct. 27, 2014, Page: 176-181
| [1] | Ivo Nikolaev Sirakov, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [2] | Raiko Dimitrov Peshev, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [3] | Tsviatko Marinov Alexandro, Bulgarian Food Safety Agency, Sofia, Bulgaria. |
| [4] | Svetla Asparuhova Dimitrova, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [5] | Yulia Nedkova Gorova, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [6] | Olga Anatolieva Deliiska, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [7] | Veneta Jankova Boyanova, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [8] | Stefan Marinov Aleksiev, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [9] | Ralitsa Atanasova Popova, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
| [10] | Zana Georgieva Madarova, National Diagnostic and Research Veterinary Medical Institute, Sofia, Bulgaria. |
Scrapie is a genetically associated neurodegenerative disease caused by the accumulation of an abnormal protein. The aim of this study was to perform genotyping of some scrapie-positive cases in sheep and the flocks they come from and to compare the effect of specific polymorphisms. It is important to examine positive cases in order to determine the possible polymorphism in the PRNP gene and its possible phenotypical appearance. Seventy-nine blood samples and 7 brain samples from scrapie affected flocks were tested. Five flocks of sheep, of different size and breed, were genotyped and the results showed that 3 of the animals in flock 1 had additional polymorphism in codon 138, nucleotide 413. In two of the animals from flock 5 there was homozygous and heterozygous polymorphism in codon 138, position 414. The VRQ allele, which is associated with high susceptibility to scrapie, was found in all the flocks, except flock number 2. Our data showed that the atypical scrapie cases are not associated with polymorphism in codon 141. Additional polymorphism was also observed at codons 137, 138, 151 and 152. Strategies for eradication of scrapie have been employed.
Keywords
Scrapie, PRNP Gene, Genotyping, Eradication Strategy
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