ISSN: 2375-3846
American Journal of Science and Technology  
Manuscript Information
 
 
Sensory Evaluation During In-Process Optimization of ‘Itugha’ Production
American Journal of Science and Technology
Vol.3 , No. 3, Publication Date: May 25, 2016, Page: 73-81
3032 Views Since May 25, 2016, 836 Downloads Since May 25, 2016
 
 
Authors
 
[1]    

Ekpe Onot O., Department of Biochemistry, College of Medical Sciences, University of Calabar, Calabar, Nigeria.

[2]    

Igile Godwin O., Department of Biochemistry, College of Medical Sciences, University of Calabar, Calabar, Nigeria.

[3]    

Eteng Mbeh U., Department of Biochemistry, College of Medical Sciences, University of Calabar, Calabar, Nigeria.

 
Abstract
 

The Fermentation of irvingia gabonensis seeds into itugha, considered more nutritious than the raw seeds from which it is produced, is a traditional technology. The quality of Itugha is usually measured by four key parameters (drawiness, taste, aroma and flavor). This study aimed at using sensory evaluation for product quality assessment during optimization, standardization and design of the fermentation flow process, with the view to ease production process and validate process reproducibility. The in-process monitoring was carried out under controlled conditions; pH, temperature, % titratable acidity and total organic acids throughout the production process. Results indicate that early stage of fermentation was initiated by Bacillus spp (pH 6-7, 30°C and 1.8% acidity of extract); intermediate stage fermentation was caused by Micrococcus and Streptococcus spp, at pH 5.6, 35-38°C and 4.4% acidity of extract. Late stage fermentation was effected by Candida tropicallis DMB 321, giving final product quality at pH 4.5-5.1, tempt 70°C as 5.4% acidity of extract, citric acid 2.4% DM, glycolic acid 1.22% DM and oxalic acid 2.98%. Sensory analysis on 9-point Hedonic Scale was used. Overall acceptability for Like Extremely was 7.5; Like very much 8.8; Like moderately 9.00 and overall acceptability ranking 72. Population t-test analysis-value was 21.18 and F-value 12.25. Progress of flavor development showed no flavour in early stage fermentation, alcoholic aroma by 3rd day (intermediate stage) and stringent spicy aroma by 6th day which became prominent after application of heat (final stage). Optimization gave desired sensory attributes that impacted positively on product quality and consumer acceptability.


Keywords
 

Fermentation, Microbes, Optimization, Irvingia Gabonensis, Itugha, Sensory Evaluation


Reference
 
[01]    

Ekott, E, J. 1994. Nutritive value of bush mango pulp (Irvingia gabonensis) An unpublished B.Sc. Thesis, University of Uyo, Uyo. Nigeria.

[02]    

Okafor, J. C. and Ujor, G. 1994. Varietal difference in Irvingia gabonensis. Paper presented at the ICRAF pre-collection meeting, IITA, Ibadan. p.5

[03]    

Ekpe, O. O., Umoh, I. B., and Eka, O. U. 2007. Effect of a Typical Rural Processing Method on Proximate Composition and Amino Acid Profile of bush mango seeds (Irvingia gabonensis). African Journal of Food Agriculture Nutrition and Development 7(1):189-192.

[04]    

FIRRO Tech. Memo 1960.

[05]    

Irvine, F. R. 1981. Woody Plants of Ghana. In: Keein, W. Y. (ed), Trees of Nigeria, Oxford: Oxford Science Publication, p.506-507.

[06]    

Schmidt, F. R. 2005. Optimization and scale up of industrial fermentation process. Applied microbial. Biotechnol., 68:425-435.

[07]    

Bibhu Prasad Panda, Mohd. Ali and Saleem Jared, 2007. Fermentation Process Optimization. Research J. of Microb. 2: 201-208.

[08]    

Stanbury, P. F., A. Whitakar and S.J. Hall. 1997. Principles of Fermentation Technology. Elsevier, London, UK.

[09]    

Ekpe, O. O. 2009. Microbiology of itugha a traditional spread obtained from fermented Irvingia gabonensis seeds (dika nuts). Nigeria Journ. Of Nutritional sciences 30 (1): 130-134.

[10]    

Ngoddy, P. O. and Onuoha, C. C. (1985). Selected problems in Yam Processing. In: Godson Osuji (ed)., Advances in Yam Research, (ed). Enugu: Biochemical Society of Nigeria, pp. 293-318.

[11]    

Holt, Y. G. (ed) 1982. The shorter bergey’s Manual of determinative bacteriology. 8th ed., Baltimore: The Williams and Wilkins Company, 120pp.

[12]    

Hunter, B.B. and Bennett, H. L. 1973. Deuteromycetes(Fungi imperfect). In: Laskin, A. I. and Lechevaliec, N. N. (eds), CRC Handbook of Microbiology, Vol. 1, Cleveland Ohio: Organismic Microbiology CRS Press, pp 405-433.

[13]    

Gerhardt, P., Murray, R. G. E., Krieg, N. R. and Philip, C. B. (1981). Manual of Methods of General Bacteriology. Washington, DC. American Society of Microbiology, 161pp.

[14]    

Biometrieux, API 1989. Apparatus and Products of Identification (API) in Microbiology.Paris: Marcyl Etoile Press, pp 69 & 80.

[15]    

Bengtsson, I. M.and Lehotay, D. C. 1996. Sample preparation with an automated robotic station for organic acid analysis by gas chromatography-mass spectrometry. Journal of chromatography B: Biomedical Applications, 685:1-7.

[16]    

A. O. A. C (2006). Official Method of Ananlysi A.O.A.C (2006). Official Method of Ananlysis. The Association of Official Analytical Chemists. 15th Edition. Washington DC, U.S.A.S

[17]    

Lennett, E.H., Spaulding, E. H., Truant, J.P. (1974). Manual of Clinical Microbiology (2nd ed). Washington DC. American Society of Microbiological Press, 970pp.

[18]    

Iso, (1985). Methods for Sensory Analysis of Foods. Part 1. General Guide to Methodology. Iso Standard 6658.

[19]    

Zapsalis, C. and Beck, R.A. (1985). Food Chemistry and Nutritional Biochemistry. New York: John Wiley Press, pp 415-504, 549-579.

[20]    

Dziezak, J. D. 1987. Food Technology., 41: 104-122.

[21]    

Lieske, B. and Konrad, G. (1994). Protein Hydrolysis- the key to meat flavouring systems. Food Reviews International, 10:287-312.

[22]    

Salami, L. I. 1990. The effect of sprouting on the nutritive qualities of millet (Pennisetum americanum) and cowpea (Vigna unquiculata) flours and their blends. Un published Ph.D Thesis, University of Nigeria, Nsukka. Nigeria.

[23]    

Yamanishi, J., Kawakami, M., Kobayashi, A., Hamanda, J. and Musalam, Y. (1989). In T.H.Parliment, R.J.McGorrin and Chi-Tang, H.O. (eds). Thermal Generation of Aroma. Washington, DC: American Chemical Society, p 310.

[24]    

Klein, Donald W., Lansing M., Harley, J. 2006. Microbiology (6th ed). New York: MaGraw-Hill. p.61.

[25]    

Anonymous, 1986. Alpine process technology Ltd. Runcorn, Cheshire.

[26]    

Cheesbrough, M. 1984. Medical Laboratory Mannual for Tropical Countries. Volume 11: Microbiology. England:ELBS Publishers, pp.66-420.

[27]    

Okafor, N. 1987. Nature of industrial microbiology, Ile-Ife, Nigeria: University of Ife Press Ltd., 413pp.

[28]    

Fellows, P. J. 1990. Food processing technology- principles and practice. (2nd edn) New York: Ellis Horwood, 493pp.

[29]    

Ekpe, O. O. and Igile, G. O. 2013. Anti-nutrient, Nutrient Composition and Nutritional Potential of itugha: A Fermented Product of Irvingia gabonensis seeds. International Journal of Biochemistry (Photon) 108:266-272.

[30]    

Lowrison, G. C. 1974. Crushing and Grinding. In: Butterworths Monographs in Chemistry and Engineering, London: Butterworths Press, 286pp.

[31]    

Prahba, T. N., Narayanan, M. S. and Partwardhan, M. V. (1990). Flavour formation in Callus cultures of Guava (Pridium guajava) Fruit. Journal of Science Food Agriculture. 50:105-110.

[32]    

Maga, J. A. 1994. Potato flavor. Food Reviews International, 10:1-48.

[33]    

Susana, Rodriquez Couto and Sanroman, M. Angeles. 2006. Application of solid-state-fermentation to food industry. A Review Journal of Food Engineering 76: 291-302.

[34]    

Osinowo, F. A. O. (1985). The flavor of Edible Yam species. In: Advances in Yam Research, Enugu: Biochemical Society of Nigeria in collaboration with Anambra State University of Technology, pp 233-248.

[35]    

Solms, T. and Wyler, R. (1979). Food Taste Chemistry, J. C. Boudreaus, (ed). American Chemical Society, Washington D. C., pp 175.

[36]    

Tung, M. A. and Stanley, D. W. (1976). Microstructure of food and its relation to Texture. Rheology and Texture in Food Quality, Connecticut, West Port: AVI., PP 28-78.

[37]    

Kennedy, M. and Krouse, D. 1999. Strategies for improving fermentation medium performance: A Review J. Ind. Microbiol. Biotechnol., 23:456-475.

[38]    

Kumar, D., Jan, V. K., Shanker, G and Srivastava, A. 2003. Citric acid production by solid state fermentation using sugarcane bagasse. Process Biochem., 38: 1731-1738.

[39]    

Ahama, M. Z., Panda, B. P., Javed, S. and Ali, M. 2006. Production of mevastatin by solid-state fermentation using wheat bran as substrate. Res. J. Microbiol., 1: 443-447.





 
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