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Physiochemical Properties Associated with Transfection of Cationic Liposome-Based Gene Delivery Vectors in the Absence of DOPE

American Journal of Science and Technology
Vol.2 , No. 4, Publication Date: Jun. 11, 2015, Page: 165-175

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Authors

[1]	Addai-Mensah Donkor, Division of Pharmaceutical Sciences, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, New York, USA; University for Development Studies, Department of Applied Chemistry & Biochemistry, Faculty of Applied Sciences, Navrongo Campus, UER, Ghana.
[2]	Machalachis Savva, Division of Pharmaceutical Sciences, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, New York, USA.

Abstract

In this article we report on novel cationic lipids and their physiochemical characteristics. Intrinsic pKa of the cationic lipidswere studied utilizing the surface charge active fluorescent probe TNS.TNS constitutes the aminonaphthaline-6-sulfonate based fluorescent dyes identified to benefit from a polarity-dependent fluorescence and have been used for long to probe structural modification in membranes. Langmuir monolayer of the novel lipids were analyzed on air/buffer interface by the film balance technique and the differential scanning calorimeter (DSC) was also explored to study the level of purity and the phase transition behavior of the lipid derivatives. The thermodynamic figures determined from ᴨ―A isotherm gave information regarding the critical temperature (Tc), which was in approximate conformity with the lipid phase transition temperature from gel-liquid state (Tm) found by both fluorescence polarization studies and differential scanning calorimetry.

Keywords

Acyl Chain Fluidity, Elasticity, TNS, Langmuir, Differential Scanning Calorimeter, Isotherm, Transition Temperature, Monolayer, Gel-Liquid State

Reference

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