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Vol.3 , No. 2, Publication Date: Jun. 13, 2017, Page: 20-24
 as Cytotoxicity (Brine Shrimp Lethality Assay)&description=<i>Eruca sativa</i> Miller of the family Brassicaceae is grown in West Asia and North Africa as poor quality oilseed crop at peripheral land under poor rainwater. Numerous human pathogenic bacteria and fungi have been subdued into drug-resistant strains. Countless synthetic antioxidant compounds have posted carcinogenic effects. This phenomenon requests further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biologically active compounds from rocket plant. In Brine Shrimp Lethality Bioassay, all the extracts produced dose-dependent cytotoxicity effect to brine shrimp nauplii with methanol extract of seed exhibiting highest toxicity. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). The final concentrations were 1000, 100, 10 and 1 ppm (μg/mL). Ten nauplii were added into (3) replicates of each concentration of the plant extract. After 24 hours the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the extracts of <i>E. sativa</i> meal (methanolic, Hexane, Heat treatment-methanol), and oil were potent against the brine shrimp with LC50 values of 91, 106, 111 and 98 ppm (μg/mL), respectively. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine. E. Sativa meal and oil have the highest of total polyphenols and flavonoids contents, which were 49.77 mg GAE/g and 11.82 mg GAE/g, respectively. Antioxidant activity of methanolic, hexane and heat-methanolic extracts and oil of <i>E. saliva</i> were determined by using FRAP, ABTS, and DPPH.&picture=http://www.aascit.org/aascit/decorators/img/journal/893.jpg)
| [1] | Husain Abd Allah El-Fadaly, Agriculture Microbiology Department, Faculty of Agriculture, Damietta University, Egypt. |
| [2] | Sherif Mohamed El-Kadi, Agriculture Microbiology Department, Faculty of Agriculture, Damietta University, Egypt. |
| [3] | Mostafa Maher El-Moghazy, Animal Production Department, Faculty of Agriculture, Damietta University, Egypt. |
| [4] | Ahmed Ali Soliman, Department of Animal Nutrition Research - Animal Production Research Institute (APRI) - Agricultural Research Center (ARC), Dokki, Giza, Egypt. |
| [5] | Mahmoud Salama Mahmoud El-Haysha, Department of Animal Nutrition Research - Animal Production Research Institute (APRI) - Agricultural Research Center (ARC), Dokki, Giza, Egypt. |
Eruca sativa Miller of the family Brassicaceae is grown in West Asia and North Africa as poor quality oilseed crop at peripheral land under poor rainwater. Numerous human pathogenic bacteria and fungi have been subdued into drug-resistant strains. Countless synthetic antioxidant compounds have posted carcinogenic effects. This phenomenon requests further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biologically active compounds from rocket plant. In Brine Shrimp Lethality Bioassay, all the extracts produced dose-dependent cytotoxicity effect to brine shrimp nauplii with methanol extract of seed exhibiting highest toxicity. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). The final concentrations were 1000, 100, 10 and 1 ppm (μg/mL). Ten nauplii were added into (3) replicates of each concentration of the plant extract. After 24 hours the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the extracts of E. sativa meal (methanolic, Hexane, Heat treatment-methanol), and oil were potent against the brine shrimp with LC50 values of 91, 106, 111 and 98 ppm (μg/mL), respectively. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine. E. Sativa meal and oil have the highest of total polyphenols and flavonoids contents, which were 49.77 mg GAE/g and 11.82 mg GAE/g, respectively. Antioxidant activity of methanolic, hexane and heat-methanolic extracts and oil of E. saliva were determined by using FRAP, ABTS, and DPPH.
Keywords
Brine Shrimp Lethality Assay (BSLA), Eruca sativa, Meal, Oil, Extracts, LC50Value, Cytotoxicity
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