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American Journal of Biomedical Science and Engineering

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Quantitative Determination of Some Secondary Metabolites and the Anti - Bacterial Effects of the Leave Extracts of Duranta eracta

American Journal of Biomedical Science and Engineering
Vol.1 , No. 5, Publication Date: Oct. 13, 2015, Page: 82-87

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Authors

[1]	A. S. Ogbuagu, Pure and Industrial Chemistry Department, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka. Anambra State, Nigeria.
[2]	K. Okoro, Pure and Industrial Chemistry Department, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka. Anambra State, Nigeria.
[3]	M. U. Akpuaka, Pure and Industrial Chemistry Department, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka. Anambra State, Nigeria.
[4]	J. O. Ogbuagu, Pure and Industrial Chemistry Department, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka. Anambra State, Nigeria.
[5]	U. E. Ekpunobi, Pure and Industrial Chemistry Department, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka. Anambra State, Nigeria.
[6]	E. C. Ohaekenyem, Pure and Industrial Chemistry Department, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka. Anambra State, Nigeria.

Abstract

Analyses were carried out on methanol and n - hexane extract of the leaves of Duranta erecta to qualitatively and quantitatively determine the presence of some secondary metabolites. The phytochemical screening showed the presence of tannins (0.49mg/100g), flavonoids (0.85mg/100g), alkaloids (0.14mg/100g) and saponins (0.78mg/100g) in the methanolic extract while the n - hexane extract showed the presence of only alkaloid (0.18mg/100g), flavonoids (1.70mg/100g and saponins (0.64mg/100g). The antibacterial activity of the extracts were evaluated against some human pathogenic bacteria; Proteus mirabilis, Proteus mirabilis, Bacillus substillis, Salmonella typhii and Bacillus aereus at 600mg/ml, 300mg/ml, 150mg/ml, and 75mg/ml by agar diffussion method. The result of the antibacterial activity showed activities against the growth of Proteus mirabilis (mic 129mg/ml), Bacillus substillis (mic 141mg/ml), Salmonella typhii (mic 81mg/ml)and Bacillus aereus (mic 100mg/ml) while the nhexane extract did not show any activity against the growth of any bacteria.

Keywords

Secondary Metabolites, MIC - minimum Inhibitory Concentration, Extract, Bacteria

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