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Vol.3 , No. 3, Publication Date: May 6, 2016, Page: 27-34
 <i>cv</i> Frida using encapsulation by sodium alginate. Different factors affecting the encapsulation efficiency such as explant type, presence of BA and presence of sucrose were studied. The best combinations for synthetic seed production found to be using of 4% sodium alginate for encapsulation of shoot tip explants in presence of 1.3 mg/l BA or 4% sucrose. In case of adventitious shoots, the best combinations found to be using of 4% sodium alginate with 1.3 mg/l BA, 4% sucrose and 1.2% agar.&picture=http://www.aascit.org/aascit/decorators/img/journal/892.jpg)
| [1] | Roba M. Ismail, Gene Transfer Lab, Plant Genetic Transformation Department, Agricultural Genetic Engineering Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. |
| [2] | Wesam M. Raslan, Gene Transfer Lab, Plant Genetic Transformation Department, Agricultural Genetic Engineering Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. |
| [3] | Gihan M. H. Hussein, Gene Transfer Lab, Plant Genetic Transformation Department, Agricultural Genetic Engineering Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. |
Synthetic or artificial seed protocol is a powerful method for production of seed analogues. It relies on in vitro encapsulation of any meristematic tissue by its coating with suitable gelling agents. Here we describe the synthetic seeds production for sugar beet (Beta vulgaris) cv Frida using encapsulation by sodium alginate. Different factors affecting the encapsulation efficiency such as explant type, presence of BA and presence of sucrose were studied. The best combinations for synthetic seed production found to be using of 4% sodium alginate for encapsulation of shoot tip explants in presence of 1.3 mg/l BA or 4% sucrose. In case of adventitious shoots, the best combinations found to be using of 4% sodium alginate with 1.3 mg/l BA, 4% sucrose and 1.2% agar.
Keywords
Artificial Seed, Sugar Beet, Adventitious Shoots Sodium Alginate
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