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Vol.2 , No. 6, Publication Date: Dec. 11, 2015, Page: 217-222
, Salna, Gazipur, to assess their response to viral diseases and horticultural qualities under natural condition during November 2010 to May 2011. Based on the visual observation of the test lines, seven (Pk13-1-1, Pk20-2-1, Pk02-2-1, Pk19-4-1, Pk54-4-12, Pk01-10-9-4 and Pk106) were showed highly resistant, four (Pk31-2-4, Pk07-4-7, Pk04-7-12-3 and BARI mistikumra 2) resistant, four (Pk55-2-2, BARI mistikumra1,Pk101 and Pk107) moderately resistant, 10 moderately susceptible and one (Pk05-1-2) susceptible against pumpkin viruses. Virus incidence and severity (Percent disease index) of test lines ranged from 0.0 to 79.9% and 0.0 to 83.3%, respectively. Four viruses were detected in those of symptomatic pumpkin leaves by Double antibody Sandwich ELISA (DAS-ELISA) techniques. Out of 26 test lines, five (Pk55-2-2, Pk05-1-2, BARI mistikumra1, BARI mistikumra 2 and Pk101) were positive to <i>Papaya ringspot virus</i> (PRSV-W); five (Pk05-4-1, Pk05-8-2, Pk75-1, Pk07-4-7 and Pk102) <i>Zucchini yellow mosaic virus</i> (ZYMV), two (Pk34-4-3 and Pk67-1-9) <i>Cucumber mosaic virus</i> (CMV), and only one (Pk105) <i>Watermelon mosaic virus</i> (WMV2). ELISA technique for assessment of virus response showed 13 lines were completely free from all of these four poty viruses. On the other hand, visual assessment technique for virus response demonstrated seven lines were completely free from viruses. It is interesting to note that visual assessment indicated six lines showed virus infection but serologically they were free from viruses suggesting these lines might be infected by other poty viruses. Significant variation was observed in the breeding lines for horticultural qualities relative to yield, yield contributing characters, fruit skin color, fruit shape. Considering virus reaction and horticultural qualities, 15 lines (Pk13-1-1, Pk20-2-1, Pk02-2-1, Pk19-4-1, Pk54-4-12, Pk01-10-9-4, Pk31-2-4, Pk04-7-12-3, Pk07-4-7, Pk55-2-2, BARI mistikumra1, BARI mistikumra 2, Pk101, Pk106 and Pk107) were selected for their improvement for virus disease resistance.&picture=http://www.aascit.org/aascit/decorators/img/journal/892.jpg)
| [1] | Begum F., Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. |
| [2] | Masud M. A. T., PSO, Vegetable Division, HRC, Bangladesh Agriculture Research Institute (BARI), Gazipur, Bangladesh. |
| [3] | Akanda A. M., Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agril University, Gazipur, Bangladesh. |
| [4] | Hossain M. B., Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. |
| [5] | Miah I. H., Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agril University, Gazipur, Bangladesh. |
Virus diseases are important problem for production of pumpkin in the world. Among the diseases, watermelon strain of Papaya ringspot virus, Zucchini yellow mosaic virus, Cucumber mosaic virus and Watermelon mosaic virus have been reported occurring in field growing pumpkin. To elucidate resistance response of pumpkin, twenty six pumpkin breeding lines were evaluated in the experimental field of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur, to assess their response to viral diseases and horticultural qualities under natural condition during November 2010 to May 2011. Based on the visual observation of the test lines, seven (Pk13-1-1, Pk20-2-1, Pk02-2-1, Pk19-4-1, Pk54-4-12, Pk01-10-9-4 and Pk106) were showed highly resistant, four (Pk31-2-4, Pk07-4-7, Pk04-7-12-3 and BARI mistikumra 2) resistant, four (Pk55-2-2, BARI mistikumra1,Pk101 and Pk107) moderately resistant, 10 moderately susceptible and one (Pk05-1-2) susceptible against pumpkin viruses. Virus incidence and severity (Percent disease index) of test lines ranged from 0.0 to 79.9% and 0.0 to 83.3%, respectively. Four viruses were detected in those of symptomatic pumpkin leaves by Double antibody Sandwich ELISA (DAS-ELISA) techniques. Out of 26 test lines, five (Pk55-2-2, Pk05-1-2, BARI mistikumra1, BARI mistikumra 2 and Pk101) were positive to Papaya ringspot virus (PRSV-W); five (Pk05-4-1, Pk05-8-2, Pk75-1, Pk07-4-7 and Pk102) Zucchini yellow mosaic virus (ZYMV), two (Pk34-4-3 and Pk67-1-9) Cucumber mosaic virus (CMV), and only one (Pk105) Watermelon mosaic virus (WMV2). ELISA technique for assessment of virus response showed 13 lines were completely free from all of these four poty viruses. On the other hand, visual assessment technique for virus response demonstrated seven lines were completely free from viruses. It is interesting to note that visual assessment indicated six lines showed virus infection but serologically they were free from viruses suggesting these lines might be infected by other poty viruses. Significant variation was observed in the breeding lines for horticultural qualities relative to yield, yield contributing characters, fruit skin color, fruit shape. Considering virus reaction and horticultural qualities, 15 lines (Pk13-1-1, Pk20-2-1, Pk02-2-1, Pk19-4-1, Pk54-4-12, Pk01-10-9-4, Pk31-2-4, Pk04-7-12-3, Pk07-4-7, Pk55-2-2, BARI mistikumra1, BARI mistikumra 2, Pk101, Pk106 and Pk107) were selected for their improvement for virus disease resistance.
Keywords
Pumpkin, Breeding lines, Viruses, Incidence, DAS-ELISA
Reference
| [01] | Agrios, G.N. 2005. Plant Pathology. 5th Edn., Academic Press, Burlington: 992, ISBN:0120 445654. |
| [02] | Bananej, K., Vahdat, A. 2008. Identification, distribution and incidence of viruses in field- grown cucurbit crops of Iran. Phytopathol. Mediterr 47: 247–257. |
| [03] | BBS (Bangladesh Bureau of Statistics). 2012. Monthly Statistics Bulletin of Bangladesh. Statistics Division. Ministry of Planning. Government of the Peoples Republic of Bangladesh. |
| [04] | Begum, S.N., Khan, M.A. 1996. Tomato Leaf curl virus in Bangladesh. Proceedings of the Phase 1 final workshop of the South Asia Veg. Res. Network, Katmandu, Nepal: 210-215. |
| [05] | Bernardo, R. 2002. Breeding for Quantitative Traits in Plants. Stemma Press, 1938 Bowsens Lane, Woodbury, MN55125, USA. |
| [06] | Bos, L. 1969. Experience with a collection of plant viruses in leaf material dried and stored over calcium chloride, and a discussion of literature on virus preservation. Genetics 34: 875-887. |
| [07] | Bos, L. 1983. Introduction to plant virology. First Edition. Centre for Agricultural Publishing and Documentation, Wageningen, the Netherlands: 160. |
| [08] | Coutts,B. A., Jones, R. A. C. 2005. Incidence and distribution of viruses infecting cucurbit Crops in the Northern Territory and Western Australia. Australian Journal of Agricultural Research 56(8): 847–858. |
| [09] | Davis, R. F., Mizuki, M. K. 1987. Detection of cucurbit viruses in New Jersey. Plant Dis.7: 40-44. |
| [10] | Köklü, G., Yilmaz, Ö. 2006. Occurrence of cucurbit viruses on field-grown melon and Water melon in the Thrace region of Turkey. Phytoprotection 87(3). |
| [11] | Krstiã, B. B., Berenji, J. B., Dukiã, N. D., Vico, I. M., Katis, N. I., Papavassiliou, C. C. 2002. Identification of Viruses Infecting Pumpkins (Cucurbita Pepo L.) in Serbia. Proceedings for Natural Sciences, Matica Srpska. Novi Sad, V: 103, 67—79. |
| [12] | Masud, M. A. T. 1995. Variability association and genetic diversity in pumpkin. M.S. Thesis, Dept. of Hort., BAU, Mymensingh. |
| [13] | Masud, M.A.T., Rashid, M.A., Rashid, M.H., Sultana, N.A., Ahmed, B., Karim, A.N.M.R.. |
| [14] | Akanda, A.M., Miller, S., Muquit, A.2009. Sources of pumpkin viris resistance in pumpkin. Bangladesh J. Plant Pathol. 25(1&2): 11-15. |
| [15] | Mendlinger, S., Chweya, J., Benzioni, A., Seme, A., Ventura, M., Lungaho, C., Okoko, V. 1991. Collection, evaluation and breeding of African edible vegetables. BGUN-ARI-25-92. Annual report 1991 on AID-CDR programme. |
| [16] | Muqit, A. 1995. Studies on virus disease of ash gourd. M.S. Thesis, Dept. of Plant Pathology, BSMRAU, Salna, Gazipur. |
| [17] | Nasiruddin, N. and Karim, M.A. 1992. Evaluation of potential control measure for fruit fly, Bactocera (Daucus) Cucurbitae, in snake gourd. Bangladesh J. Ent. 2(1 &2): 31-34. |
| [18] | Noordam, D. 1973. Identification of plant viruses–methods and experiments. Centre of Agri cultural publishing and Documentation, Wageningen, the Netherlands : 207. |
| [19] | Papayiannis, L. C., Ioannou, N., Boubourakas, I. N. Dovas,C. I., Katis, N. I., Falk, B. W. 2005. Incidence of Viruses Infecting Cucurbits in Cyprus. Journal of Phytopathol. 153(9):530–535. |
| [20] | Paper, J.K., Handley, M.K., Kulakow, P.A. 1996. Incidence and severity of viral disease symptoms on eastern gamagrass within monoculture and polycultures. Elsevier. Agric. Ecosyst. Environ. 59: 139-147. |
| [21] | Provvidenti, R. 1996. Diseases caused by viruses. p. 37-45. In: Zitter, T. A., Hopkins,D. L., Thomas,C. E. (Eds.). Compendium of Cucurbit Diseases. PS Press, St. Paul, Minn. |
| [22] | Rahman, M.M, Dey, S.K., Wazuddin, M. 1990. Yield, yield components and plant characters of several bitter gourds, ribbed gourd, bottle gourd and sweat gourd genotypes. BAU Res. Progress. 4: 117-127. |
| [23] | Rana, T. K., Vashistha, R. N., Pandita, M. L. 1986. Genetic variability studies in pumpkin (Cucurbita moschata Poir.). Haryana J. Hort. Sci. 15(1-2): 71-75. |
| [24] | Riechmann, J. L., Lain,S., Garcia, J. A. 1992. Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 73:1-16. |
| [25] | Roy, D.K. 1993. Studies on the prevalence and some properties of bottle gourd mosaic virus. A thesis submitted to the Department of Plant Pathology, IPSA, and Bangladesh for the partial fulfillment of the MS degree: 51. |
| [26] | Shaifullah, S.M.K. 2003. Virus infecting pumpkin in Southern Bangladesh. MS Thesis, Dept. of Plant Pathology, BSMRAU, Salna, Gazipur: 64. |
| [27] | Somowiyarjo, S., Sako, N. and Tomaru, K. 1993. The use of dot immonobinding assay for detecting cucurbit viruses in Yogyakarta. P. 3-11. In: Tomaru, K. and K.T.N atsuaki (editors). Production of virus free tropical crops. NODAI Centre for International Programs. Tokyo University of Agriculture, Tokyo. |
| [28] | Sreenivasulu, P., Naidu, R.A., Nayudu, M. V. 1989. Physiology of virus-infected plants. South Asian Publishers Pvt. Ltd. New Delhi, India: 164. |
| [29] | Strange, E.B., Guner, N., Esbroeck, Z.P.V., Wehner, T.C. 2002. Screening the watermelon germplasm collection for resistance to Papaya ringspot virus type-W. Crop Sci. 42: 1324 -1330. |
| [30] | Vijoy, O.P. 1987. Genetic variability and path-analysis in muskmelon (Cucumis melo). Indian Journal Horticulture 44(4): 233-238. |
| [31] | Vincelli, P., Seebold, K. 2009. Report of a Watermelon mosaic potyvirus strain in Kentucky undetected by ELISA. Online. Plant Hearth Progress doi: 10.1094/PHP-2009-0313-01-BR. |
| [32] | Xu Y.,Kang, D. Shi, Z. Shen, H., Wehner, T. 2004. Inheritance of Resistance toZucchini Yellow Mosaic Virus and Watermelon Mosaic Virus in Watermelon. Journal of Heredit. 95(6): 498–502. |
| [33] | Zitter, T.A., Hopkins, D.L, Thomas, C.E. 1996. Compendium of cucurbit diseases. APS Press, St. Paul, MN. |
