AASCIT - Journal - All Issues

ISSN Print: 2472-9574 ISSN Online: 2472-9590

International Journal of Chemical and Biomedical Science

The Journal

Manuscript Information

A Fast and Simple Quantitative Profiling Method of Endogenous Saccharides in Rat Urine Using HILIC-MS/MS

International Journal of Chemical and Biomedical Science
Vol.4 , No. 4, Publication Date: May 30, 2018, Page: 54-59

2649 Views Since May 9, 2018, 901 Downloads Since May 9, 2018

Paper in PDF (964K)

Follow on

Authors

[1]	Gang Cheng, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[2]	Bangjie Zhu, Department of Chemistry, East China Normal University, Shanghai, China.
[3]	Feng Liu, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[4]	Yan Wang, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[5]	Yu Cheng, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[6]	Chao Yan, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

Abstract

To explore the metabolism of endogenous saccharides in rat, we developed a simple, fast, simultaneously quantitative method for the endogenous saccharides in rat urine, using the hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry. In order to quantify 15 endogenous saccharides in rat urine, various conditions, including columns, chromatographic conditions, MS conditions, and urine preparation methods, were investigated and optimized. The reproducibility, precision, recovery, and stability of the method were verified. The results indicated that 5 times volume of cold organic solvents (methanol/acetonitrile, v/v, 50/50) coupled with vortex for 1 min and incubated at -20C for 20 min were the most optimal conditions for extraction. The results, according to the linearity, precision, recovery, matrix effect and stability, showed that the method was satisfactory in the quantification of endogenous saccharides in rat urine. Meeting the requirement of quantification in specific expanded metabolomic studies, the quantified analysis of endogenous saccharides in rat urine performed excellently in the sensitivity, high throughput and simple sample preparation.

Keywords

Endogenous Saccharides, Hydrophilic Interaction Chromatography, HILIC-UPLC-MS/MS, Rat Urine

Reference

[01]	Dai, Z. W., et al., Metabolic profiling reveals coordinated switches in primary carbohydrate metabolism in grape berry (Vitis vinifera L.), a non-climacteric fleshy fruit. J Exp Bot, 2013. 64 (5): p. 1345-55.
[02]	Liao, W., et al., Metabonomic variations associated with AOM-induced precancerous colorectal lesions and resveratrol treatment. J Proteome Res, 2012. 11 (6): p. 3436-48.
[03]	Cheng, Y., et al., Distinct urinary metabolic profile of human colorectal cancer. J Proteome Res, 2012. 11 (2): p. 1354-63.
[04]	Huang, X. X., H. Q. Zhou, and H. Zhang, The effect of Sargassum fusiforme polysaccharide extracts on vibriosis resistance and immune activity of the shrimp, Fenneropenaeus chinensis. Fish & Shellfish Immunology, 2006. 20 (5): p. 750-757.
[05]	Neuschwander-Tetri, B. A., Carbohydrate intake and nonalcoholic fatty liver disease. Current Opinion in Clinical Nutrition and Metabolic Care, 2013. 16 (4): p. 446-452.
[06]	Ackerman, Z., et al., Fructose-induced fatty liver disease: hepatic effects of blood pressure and plasma triglyceride reduction. Hypertension, 2005. 45 (5): p. 1012-8.
[07]	Chen, W. L., et al., Enhanced Fructose Utilization Mediated by SLC2A5 Is a Unique Metabolic Feature of Acute Myeloid Leukemia with Therapeutic Potential. Cancer Cell, 2016. 30 (5): p. 779-791.
[08]	Gervasoni, J., et al., Validation of an LC-MS/MS Method for Urinary Lactulose and Mannitol Quantification: Results in Patients with Irritable Bowel Syndrome. Dis Markers, 2016. 2016: p. 5340386.
[09]	Dong, Y., et al., A modified LC-MS/MS method to simultaneously quantify glycerol and mannitol concentrations in human urine for doping control purposes. J Chromatogr B Analyt Technol Biomed Life Sci, 2016. 1022: p. 153-158.
[10]	Harvey, D. J., Derivatization of carbohydrates for analysis by chromatography; electrophoresis and mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 2011. 879 (17-18): p. 1196-225.
[11]	Ruiz-Aceituno, L., et al., Development of a carbohydrate silylation method in ionic liquids for their gas chromatographic analysis. Analytica Chimica Acta, 2013. 787 (0): p. 87-92.
[12]	Becker, M., et al., Evaluation of different derivatisation approaches for gas chromatographic-mass spectrometric analysis of carbohydrates in complex matrices of biological and synthetic origin. J Chromatogr A, 2013. 1281: p. 115-26.
[13]	Ruiz-Matute, A. I., M. L. Sanz, and I. Martinez-Castro, Use of gas chromatography-mass spectrometry for identification of a new disaccharide in honey. J Chromatogr A, 2007. 1157 (1-2): p. 480-3.
[14]	Albalasmeh, A. A., A. A. Berhe, and T. A. Ghezzehei, A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydrate Polymers, 2013. 97 (2): p. 253-261.
[15]	Dvořáčková, E., M. Šnóblová, and P. Hrdlička, Carbohydrate analysis: From sample preparation to HPLC on different stationary phases coupled with evaporative light-scattering detection. Journal of Separation Science, 2014. 37 (4): p. 323-337.
[16]	Terol, A., et al., Rapid and sensitive determination of carbohydrates in foods using high temperature liquid chromatography with evaporative light scattering detection. Journal of Separation Science, 2012. 35 (8): p. 929-936.
[17]	Karlsson, G., S. Winge, and H. Sandberg, Separation of monosaccharides by hydrophilic interaction chromatography with evaporative light scattering detection. J Chromatogr A, 2005. 1092 (2): p. 246-9.
[18]	1Zhu, B., Wei, H., Wang, Q., Li, F., Dai, J., Yan, C., Cheng, Y., A simultaneously quantitative method to profiling twenty endogenous nucleosides and nucleotides in cancer cells using UHPLC-MS/MS. Talanta 2018, 179, 615-623.
[19]	Jahouh, F., Wang, R., Simultaneous quantification of labeled (2) H5-glycerol, (13) C6-glucose, and endogenous D-glucose in mouse plasma using liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 2015, 407 (28), 8617-22.
[20]	Perry, R. J., Borders, C. B., Cline, G. W., Zhang, X. M., Alves, T. C., Petersen, K. F., Rothman, D. L., Kibbey, R. G., Shulman, G. I., Propionate Increases Hepatic Pyruvate Cycling and Anaplerosis and Alters Mitochondrial Metabolism. J Biol Chem 2016, 291 (23), 12161-70.
[21]	Vilhena, R. D., et al., A new HILIC-MS/MS method for the simultaneous analysis of carbidopa, levodopa, and its metabolites in human plasma. J Chromatogr B Analyt Technol Biomed Life Sci, 2014. 967C: p. 41-49.
[22]	Trivedi, D. K. and R. K. Iles, HILIC-MS-based shotgun metabolomic profiling of maternal urine at 9-23 weeks of gestation - establishing the baseline changes in the maternal metabolome. Biomed Chromatogr, 2014.
[23]	Yao, X., et al., HILIC-UPLC-MS/MS combined with hierarchical clustering analysis to rapidly analyze and evaluate nucleobases and nucleosides in Ginkgo biloba leaves. Drug Test Anal, 2014.
[24]	Matuszewski, B. K., M. L. Constanzer, and C. M. Chavez-Eng, Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Analytical Chemistry, 2003. 75 (13): p. 3019-3030.
[25]	Zhou, W., et al., A rapid and simple method for the simultaneous determination of four endogenous monoamine neurotransmitters in rat brain using hydrophilic interaction liquid chromatography coupled with atmospheric-pressure chemical ionization tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 2015. 1002: p. 379-86.
[26]	Kinoshita, K., S. Jingu, and J. Yamaguchi, A surrogate analyte method to determine D-serine in mouse brain using liquid chromatography-tandem mass spectrometry. Analytical Biochemistry, 2013. 432 (2): p. 124-130.