World Journal of Biochemistry and Molecular Biology  
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Thiacalix[4]arene-tetraphosphonate Eliminates Inhibitory Effects of Heavy Metals on Smooth Muscle Myosin S1 ATPase Activity
World Journal of Biochemistry and Molecular Biology
Vol.3 , No. 2, Publication Date: May 16, 2018, Page: 46-54
2246 Views Since May 16, 2018, 485 Downloads Since May 16, 2018
 
 
Authors
 
[1]    

Raisa Labyntseva, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

[2]    

Viktoriia Yavorovska, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

[3]    

Alexander Bevza, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

[4]    

Andriy Drapailo, Department of Phosphorane Chemistry, Institute of Organic Chemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

[5]    

Vitaly Kalchenko, Department of Phosphorane Chemistry, Institute of Organic Chemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

[6]    

Sergiy Kosterin, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

 
Abstract
 

Numerous female reproductive abnormalities are caused by uterine smooth muscle (myometrium) dysfunctions. Heavy metals have an adverse effect on the contractility of uterine smooth muscle. Thus, methods recovering normal contractile activity of myometrium are needed to be developed to overcome this negative impact. It has been found an inhibitory effect of Ni2+, Pb2+, and Cd2+ on enzymatic hydrolysis of ATP catalyzed by myosin subfragment-1 (S1) obtained from smooth muscle of swine uterus. It was demonstrated that tetrahydroxythiacalix[4]arene-tetraphosphonate (C-800) restored the normal myosin S1 ATPase activity in the presence of heavy metal cations. One of the most probable mechanisms of tetrahydroxythiacalix[4]arene-tetraphosphonate protective effect is based on its ability to chelate heavy metal cations from the incubation medium. Also, we speculated that protective activity of C-800 might be the result of weakening the interaction between heavy metal ions and amino acid residues near the active site of myosin ATPase.


Keywords
 

Myosin S1, Heavy Metals, Thiacalix[4]arene, ATPase Activity, Docking, Smooth Muscle, Uterus


Reference
 
[01]    

Rzymski P, Tomczyk K (2015) Impact of heavy metals on the female reproductive system. Annals of Agricultural and Environmental Medicine. 22: 259–264.

[02]    

Sengupta P; Banerjee R; Nath S; Das S; Banerjee S. (2015) Metals and female reproductive toxicity. Hum Exp Toxicol. 34, 7, 679-97.

[03]    

Thompson J, Bannigan J (2008) Cadmium: Toxic effects on the reproductive system and the embryo. Reprod Toxicol. 25: 304–315.

[04]    

Rahman A, Kumarathasan P, Gomes J (2016) Infant and mother related outcomes from exposure to metals endocrine disrupting properties during pregnancy. Science of the Total Environment. 1: 569-570.

[05]    

Ajayi OO (2012) Progesterone, selected heavy metals and micronutrients in pregnant Nigerian women with a history of recurrent spontaneous abortion. African Health Sciences. 12: 153-159.

[06]    

Lei LL, Wei HJ (2015) Relationship between risk factors for infertility in women and lead, cadmium, and arsenic blood levels: a cross-sectional study from Taiwan. BMC Public Health. 15: 12-20.

[07]    

Nagata C, Nagao Y (2005) Urinary Cadmium and serum levels of estrogens and androgens in postmenopausal Japanese women. Cancer Epidemiology, Biomarkers & Prevention. 14: 705-708.

[08]    

Rzymski P, Niedzielski P (2016) Metal accumulation in the human uterus varies by pathology and smoking status. Environment and Epidemiology. 105: 1511-1518.

[09]    

Marx SK, Rashid S, Stromsoe N (2016) Global-scale patterns in anthropogenic Pb contamination reconstructed from natural archives. Environmental Pollution. 213: 283-298.

[10]    

Tchounwou PB, Yedjou CG (2012) Heavy Metals Toxicity and the Environment, Molecular. Clinical and Environmental Toxicology. 101: 133-164.

[11]    

Baldwin DR, Marshall WJ (1999) Heavy metal poisoning and its laboratory investigation. Ann Clin Biochem. 36: 267-300.

[12]    

Jackson LW, Zullo MD (2008) The association between heavy metals, endometriosis and uterine myomas among premenopausal women. Human Reproduction. 23: 679–687.

[13]    

Kim HS, Kim YJ, Seo YR (2015) An overview of carcinogenic heavy metal: molecular toxicity mechanism and prevention. Journal of Cancer Prevention. 20: 232-240.

[14]    

Vaktskjold, A.; Talykova, LV; Chashchin, VP; Odland, JO; Nieboer, E. (2008) Spontaneous abortions among nickel-exposed female refinery workers. Int. J. Environmental Health Research. 18 (2): 99–115.

[15]    

Preller, M; Manstein, DJ. (2012) Myosin motors: structural aspects and functionality, in reference module in life sciences. Comprehensive Biophysics. 4: 118-150.

[16]    

Labyntsevа RD, Bevza OV (2014) Protective effect of thiacalix[4]arene-tetrasulphonate on heavy metal inhibition of myometrium myosin subfragment-1 ATP-hydrolase activity. Ukr Biochem J. 86: 154-166.

[17]    

Labyntseva RD, Bobrowska OM (2011) Effect of heavy metal cations on ATPase activity of actomyosin complex and myosin subfragment-1 of the smooth muscle of the uterus. Ukr Biochem J. 83: 79-88.

[18]    

Gutsche CD Calixarenes: an introduction, monographs in supramolecular chemistry. Cambridge: Royal Society of Chemistry. 2008: 282.

[19]    

Morohashi N, Narumi F (2006) Thiacalixarenes. Chem Rev 106: 5291-5316.

[20]    

Lumetta GJ, Rogers RD, Gopalan AS. Calixarenes for separations. Washington: American Chemical Society. 2000: 366.

[21]    

Iki N, Morohashi N, Narumi F, Miyano S. High (1998) Complexation Ability of Thiacalixarene with Transition Metal Ions. The Effect of Replacing Methylene Bridges of Tetra(p-t-butyl)calyx[4]arenetetrol by Epithio Groups. Bull Chem So Jpn. 71: 1597-1603.

[22]    

Veklich TO, Shkrabak OA (2014) Kinetics of the inhibitory effect of calix[4]arene C-90 on the activity of transporting plasma membrane Ca2+, Mg2+-ATPase of smooth muscle cells. Ukr Biochem J. 86: 37-46.

[23]    

Cherenok S, Vovk A, Muravyova I (2006) Calix[4]arene α-aminophosphonic acids: asymmetric synthesis and enantioselective inhibition of alkaline phosphatases. Org. Letters. 8: 549-552.

[24]    

Trush VV, Kharchenko SG, Tanchuk VYu, Kalchenko VI, Vovk AI (2015) Phosphonate monoesters on a thiacalix[4]arene framework as potential inhibitors of protein tyrosine phosphatase 1B. Organic & Biomolecular Chemistry. 13: 8803-8806.

[25]    

Nimse SB, Kim T (2013) Biological applications of functionalized calixarenes. Chem Soc Rev. 42: 366-386.

[26]    

Konczyk J (2016) Calixarene-based extractants for heavy metal ions removal from aqueous solutions. Sep Sci Technol. 51: 2394–2410.

[27]    

Zaghbani A (2008) Thiacalix[4]arene derivatives as extractants for metal ions in aqueous solutions: Application to the selective facilitated transport of Ag(I). Materials Science and Engineering: C 28: 985-989.

[28]    

Phan G, Semili N (2013) Calixarene cleansing formulation for uranium skin contamination. Health Phys. 105: 382-389.

[29]    

Suzuki H, Kondo Y (1988) Effects of Phosphorylation, MgATP, and ionic strength on the rates of papain degradation of heavy and light chains of smooth muscle heavy meromyosin at the S1-S2 junction. J Biol Chem. 263: 10974–10979.

[30]    

Schagger H., von Jagow G. I. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368–379.

[31]    

Chen PS, Toribara J, Warner H (1956) Microdetermination of phosphorus. Anal Chem. 28: 1756–1758.

[32]    

Cassidy CE, Setzer WN (2010) Cancer­relevant biochemical targets of cytotoxic Lonchocarpus flavonoids: a molecular docking analysis. J Mol Model. 16: 311­326.

[33]    

Houdusse A, Kalabokis VN, Himmel D (1999) Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head. Cell. 97: 459­470.

[34]    

Li L, Jose J, Xiang Y (2010) Structural changes of envelope proteins during alphavirus fusion. Nature. 468: 705­708.

[35]    

Burghardt TP, Neff KL (2010) Myosin individualized: single nucleotide polymorphisms in energy transduction. BMC Genomics. 11: 172.

[36]    

Risal D, Gourinath S, Himmel DM (2004) Myosin subfragment 1 structures reveal a partially bound nucleotide and a complex salt bridge that helps couple nucleotide and actin binding. PNAS. 10: 8930­8935.

[37]    

Bugaenko LT, Ryabykh SM, Bugaenko AL. (2008) A Nearly Complete System of Average Crystallographic Ionic Radii and Its Use for Determining Ionization Potentials. Mosc. Univer. Chem. Bull. 63 (6): 303–317.

[38]    

Sigel H (2004) Adenosine 5′-triphosphate (ATP4-): Aspects of the coordination chemistry of a multitalented biological substrate. Pure Appl Chem. 76: 375–388.





 
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