World Journal of Biochemistry and Molecular Biology  
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Thiacalix[4]arene-tetraphosphonate Eliminates Inhibitory Effects of Heavy Metals on Smooth Muscle Myosin S1 ATPase Activity
World Journal of Biochemistry and Molecular Biology
Vol.3 , No. 2, Publication Date: May 16, 2018, Page: 46-54
3512 Views Since May 16, 2018, 666 Downloads Since May 16, 2018

Raisa Labyntseva, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.


Viktoriia Yavorovska, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.


Alexander Bevza, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.


Andriy Drapailo, Department of Phosphorane Chemistry, Institute of Organic Chemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.


Vitaly Kalchenko, Department of Phosphorane Chemistry, Institute of Organic Chemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.


Sergiy Kosterin, Department of Muscle Biochemistry, Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine.


Numerous female reproductive abnormalities are caused by uterine smooth muscle (myometrium) dysfunctions. Heavy metals have an adverse effect on the contractility of uterine smooth muscle. Thus, methods recovering normal contractile activity of myometrium are needed to be developed to overcome this negative impact. It has been found an inhibitory effect of Ni2+, Pb2+, and Cd2+ on enzymatic hydrolysis of ATP catalyzed by myosin subfragment-1 (S1) obtained from smooth muscle of swine uterus. It was demonstrated that tetrahydroxythiacalix[4]arene-tetraphosphonate (C-800) restored the normal myosin S1 ATPase activity in the presence of heavy metal cations. One of the most probable mechanisms of tetrahydroxythiacalix[4]arene-tetraphosphonate protective effect is based on its ability to chelate heavy metal cations from the incubation medium. Also, we speculated that protective activity of C-800 might be the result of weakening the interaction between heavy metal ions and amino acid residues near the active site of myosin ATPase.


Myosin S1, Heavy Metals, Thiacalix[4]arene, ATPase Activity, Docking, Smooth Muscle, Uterus


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