American Journal of Civil and Environmental Engineering  
Manuscript Information
Modeling the Bioremediation of Diesel Contaminated Soil by Bacteria from Clogged Drainage System
American Journal of Civil and Environmental Engineering
Vol.4 , No. 2, Publication Date: Jul. 5, 2020, Page: 11-21
451 Views Since July 5, 2020, 114 Downloads Since Jul. 5, 2020

Ejikeme Ugwoha, Department of Civil & Environmental Engineering, University of Port Harcourt, Port Harcourt, Nigeria.


Okechukwu Loius Iwuchukwu, Department of Civil & Environmental Engineering, University of Port Harcourt, Port Harcourt, Nigeria.


The contamination of soil with petroleum products is a major environmental issue. The widespread application of diesel in human activities makes it one of the most hazardous petroleum products. Among the available remediation methods, bioremediation has become the main choice for petroleum products contaminated site recovery due to its cost-effectiveness and environmental-friendliness. In this study, the bioremediation of diesel contaminated soil by bacteria from clogged drainage was examined and modeled. Soil samples were contaminated with diesel and inoculated with cultured bacteria isolated from clogged drainage systems for 56 days. Experimental results indicated that Pseudomonas, Micrococcus, Acinetobacter, Bacillus cereus and Providencia species actively participated in the bioremediation process. The percentage reduction of diesel was statistically highly significant (p<0.05) for all five bacterial species and found to be in the following order: Pseudomonas (92.39%) > Acinetobacter (88.29%) > Bacillus cereus (88.11%) > Micrococcus (86.91%) > Providencia (29.64%). The biodegradation data complied with first-order kinetic model. Thus, first-order kinetic models of the biodegradation of diesel in soil for the five active bacteria were developed. The models were used to fit the biodegradation of diesel in soil with correlation coefficient (R2) range of 0.8142 – 0.9599 and p-value range of 0.2879 – 0.8211 (p>0.05), indicating good agreement between the measured and predicted biodegradation of diesel in soil. Therefore, it is concluded that the developed models can adequately predict the biodegradation of diesel in soil with time by the respective bacteria.


Modeling, Bacteria, Biodegradation, Clogged Drainage Systems, Bioremediation, Diesel, Contaminated Soil


U.S. Energy Information Administration: Diesel fuel explained. Use of diesel. (2019). Accessed 29 March 2019.


Kaur, J., Adamchuk, V. I., Whalen, J. K., Ismail, A. A.: Development of an NDIR CO2 sensor-based system for assessing soil toxicity using substrate-induced respiration. Sensors, 15 (3), 4734–4748 (2015).


Muratova, A. Y., Golubev, S. N., Dubrovskaya, E. V., Pozdnyakova, N. N., Panchenko, L. V., Pleshakova, E. V., Chernyshova, M. P., Turkovskaya, O. V.: Remediating abilities of different plant species grown in diesel-fuel-contaminated leached chernozem. Appl. Soil Ecol., 56, 51–57 (2012).


Hewelke, E., Szatyłowicz, J., Hewelke, P., Gnatowski, T., Aghalarov, R.: The Impact of Diesel Oil Pollution on the Hydrophobicity and CO2 Efflux of Forest Soils. Water Air Soil Pollut., 229 (51), 1-11 (2018).


Klamerus-Iwan, A., Błońska, E., Lasota, J., Kalandyk, A., Waligórski, P.: Influence of oil contamination on physical and biological properties of forest soil after chainsaw use. Water Air Soil Pollut., 226 (389), 1-9 (2015).


Rodríguez-Rodríguez, N., Rivera-Cruz, M. C., Trujillo-Narcía, A., Almaráz-Suárez, J. J., Salgado-García, S.: Spatial distribution of oil and biostimulation through the rhizosphere of Leersia hexandra in degraded soil. Water Air Soil Pollut., 227 (319), 1-14 (2016).


Doerr, S. H., Shakesby, R. A., Walsh, R.: Soil water repellency: its causes, characteristics and hydro-geomorphological significance. Earth-Sci. Rev., 51 (1), 33–65 (2000).


Juwarkar, A. A., Singh, S. K., Mudhoo, A. A.: Comprehensive overview of elements in bioremediation. Rev. Environ. Sci. Biotechnol., 9 (3), 215–288 (2010).


Caliman, F. A., Robu, B. M., Smaranda, C., Pavel, V. L., Gavrilescu, M. Soil and groundwater cleanup: benefits and limits of emerging technologies. Clean Technol. Envir., 13 (2), 241–268 (2011).


Science Monk: Bioremediation- Types, Factors, Advantages, & Limitations. (2019). Accessed 13 April 2019.


ESD Waste2Water: What Is Bioremediation and What Are Its Benefits? (2018). Accessed 13 April 2019.


Blom, K.: Drainage systems, an occluded source of sanitation related outbreaks. Blom Archives of Public Health, 73: 8 (2015).


Ugwoha, E., Momoh, Y. O. L. and Iwuchukwu, O. L. Assessment of Bacterial Loads in Clogged Drainage Systems in Urban City for the Remediation of Petroleum Polluted Soil. IOSR-JESTFT, 13 (10), 58-66 (2019).


Chen-Charpentier, B. M., Kojouharov, H. V.: Mathematical modeling of bioremediation of trichloroethylene in aquifers. Comput. Math Appl., 56, 645–656 (2008).


Smith, H.: Modeling Microbial Populations in the Chemostat. School of Mathematical and Statistical Sciences, Arizona State University (2014).


Sonali, B.: Sewage Bacteria - Strictly Anaerobic, Aerobic and Facultative bacteria. (2011). Accessed 13 April 2019.


Ogbonna, D. N., NiaBari, Q. W.: The characteristics of bacteria from clogged drainage system around Rivers State University premises. An unpublished undergraduate research study, Department of Applied and Environmental Biology, Rivers State University, Port Harcourt (2017).


Mentzer, E., Ebere, D.: Remediation of hydrocarbon contaminated sites. Proceedings of the 8th Biennial International Seminar on the Petroleum Industry and Nigerian Environment, November 17-21, 1996, Port Harcourt, Nigeria (1996).


Zhongyun, M. A.: Bioremediation of petroleum hydrocarbon Contaminated soil using indigenous cultures. Master Degree Thesis, Faculty of Engineering & Applied Science, Memorial University of Newfoundland (1998).


Darsa, K. V., Thatheyus, A. J., Ramya, D.: Biodegradation of Petroleum Compound Using the Bacterium Bacillus subtilis. Curr. Sci. Int., 2 (1), 20-25 (2014).


Yudono, B., Said, M., Sabaruddin, Napoleon, A., Fanani, Z.: Kinetics Approach of Biodegradation of Petroleum Contaminated Soil by using Indigenous Isolated Bacteria. Journal of Tropical Soils, 16, 33-38 (2011).


Balba, M. T., Al-Awadhi, N., Al-Daher, R.: Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluation. J. Microbiol. Methods, 32, 155–164 (1998).


Kensa, V. M.: Bioremediation - an overview. © EM International. J. Ind. Poll. Control, 27 (2), 161-168 (2011).


Nakhla, G., Liu, V., Bassi, A.: Kinetic modeling of aerobic biodegradation of high oil and grease rendering wastewater. Bioresour. Technol., 97, 131–139 (2006).


Balseiro-Romero, M., Monterroso, C., Kidd, P. S., Gkorezis, P., Vangronsveld, J., Casares J. J.: Modeling the bioremediation of a diesel contaminated soil using an enriched hydrocarbon-degrading inoculant. Conference Paper. (2017). Accessed 13 April 2019.


Banks, H. T., Davidian, M.: Introduction to the Chemostat. MA-ST 810 North Carolina State University (2009).


Greene, E. A., Kay, J. G, Jaber, K., Stehmeier, G., Voordouw, G.: Composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons. Appl. Environ. Microbiol., 66 (12), 5282-5289 (2000).

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