Rapid Diagnosis by Multiplex PCR Technology at Patients with Acute Respiratory Infections
Introduction: PCR offer a number of potential advantages, results are available in a matter of hours rather than days, the extreme sensibility facilitates detection of even minutes the amounts of pathogen DNA in clinical samples and the test is not significantly affected by prior administration of antibiotics. Aim: The aim of this work was to rapidly identify the antibiotic resistance the monitoring of pathogen growth at the patients admitted in Hospitalization Intensive Care Unit of Emergency County Hospital Targu Jiu starting in December/2013. Method: The Analyzer Unyvero™ Pneumonia Application was used in detection of pneumonia associated pathogens and their antibiotic resistance genes using the Unyvero™ System following PCR, pathogen species with sequencing of the amplified microbial DNA. Pneumonia Cases Report December/2013: –1. Gender male, age 72 years, Sample ID 1311_1, the results was positive for Streptococcus pneumonia(873), Moraxela cataralis (308), Resistance markers of the Unyvero™ Pneumonia Panel: -tem β-Lactams gram-negative bacteria, -ermB Makrolides / Lincosamides, mecA Oxacillin Staphylococcus. Therapeutic failure must be considered when is administering Makrolides / Lincosamides(ermB), Macrolides (mefA), Penicilins (tem), [Figure 1, 2, 3]. -2. Gender male, age 65 years, Sample ID 1310_1, the results was positive for Klebsiella pneumonia(62), resistance marker result ermB ermB Makrolides / Lincosamides. Therapeutic failure must be considered when is administering Penicilins. -3. Gender Female age 55 years, Sample ID 1310_2, the results was positive Staphylococcus Aureus, tem β-Lactams gram-negative bacteria, -ermB Makrolides. Therapeutic failure must be considered when is administering Penicilins. Conclusions: The Unyvero™ results were available 2 days before the primary microbiology report and 3 days before the final confirmation results, obtained by microbiology culture. The Unyvero Analyzer only provides rapid data to support the therapeutic decision of currant medic.
Antibiotic Resistance, PCR, Gene, AND, Resistance Markers, Microbiology Report
Caliendo AM. Multiplex PCR and emerging technologies for the detection of respiratory pathogen.Clin Infect 2011; (4):5326-5330.
Rulls© Curetis AG, 2013 Unyvero ™ P50 Pneumonia Application Guide 00118 V2.0, Hamburg, Germany, 2013, http://www.curetis.com/ .
Kugelberg et al. Antimicrobials, Resistance J Antimicrob Chemother 2005; 55(1): 1-5
Yu et al. Antimicrobials, Resistance J ClinMicrobiol 2004; 42(9): 1-4
VanHoek A, Mevius D, Guerra B, Mullany P and al. Acquired antibiotic resistance genes a new review article published 2011; 1: 1-28.
Depardieu A et al. Frontiers in Microbiology. Antimicrobials, Resistance and Chemotherapy. 2011;203: 1-13.
Wright G.D. Bacterialresis- tancetoantibiotics:enzymatic degradationandmodification. Adv. DrugDeliv.Rev 2005; 57: 1451–1470.
Roberts M.C. Update on macrolide-lincosamide, treptogramin, ketolide,and oxazolidinone resistance genes, -ermA, ermB, ermC Makrolides / Lincosamides, gyrA83 of E. coli . FEMS Microbiol.Lett 2008; 282: 147–159.
Bonnet et al. ctx-M 3rdGen, Cephalosporines, Penicillines, Antimicrob Agents. Chemother 2001; 45(8): 1-8.
Pérez-Pérez et al. dha 3rdGen. Cephalosporines, J Clin Microbiol 2002; 40(6): 1-6.
Pérez-Pérez et al. ebc 3rd Gen. Cephalosporines Pérez-Pérez et al, J Clin Microbiol 2002; 40(6): 1-6.
Oliviera JE, Silva CA, Oliviera GM, Zaneta DM et al. Antimicrob Agents. Chemoter 2009; 53(7): 2887-289.