The aim of this study was to evaluate the effects of ethanolic extract of H. sabdariffa and 2, 4-dinitrophenylhydrazine on the kidney function parameters of rats. 20 rats were evenly distributed into 4 groups and treatment lasted for a week. For the first six (6) days, the animals in group one (1) and group four (4) were treated with water alone while groups two (2) and three (3) rats received ethanolic extract of H. sabdariffa alone. At the end of the 6th day, the animals were fasted overnight and on the 7th day, each animal in groups 3 and 4 received 28 mg per kg body weight of 2, 4-dinitrophenylhydrazine (2, 4-DNPH) alone and after 7 hours they were sacrificed under diethyl ether anesthesia and their serum and kidney samples taken and prepared for biochemical assays to determine their urea, sodium, potassium, bicarbonates, chloride, creatinine and malondialdehyde levels. The high levels of MDA and creatinine observed in the serum of the rats treated with 2, 4-dinitrophenylhydrazine alone relative to their respective controls show the ability of 2, 4-dinitrophenylhydrazine to provoke cytotoxicity in the kidney. It can also be established from this study that the ethanolic extract of H. sabdariffa has anti-oxidant property which was shown in the significant reduction in the level of MDA and creatinine in the serum of rats treated with the ethanolic extract H. sabdariffa prior to the treatment with 2, 4-dinitrophenylhydrazine when compared to the rats treated with 2, 4-dinitrophenylhydrazine alone.
Allen, P. J. (2012). “Creatine metabolism and psychiatric disorders: Does creatine supplementation have therapeutic value’’. Neurosci Biobehav Rev 36 (5): 1442-62.
Balami, A. (1998). The effect of processing conditions, packaging and storage on selected quality attributes of Mungza Ntuza (M. Sc. thesis), University of Ibadan, Nigeria.
Clemens, M. R., Reinmer, H and Waller, H. D. (1984). Phenylhydrazine-induced Lipid peroxidation of Red blood cells In vitro and In vivo: Monitoring by the the production of volatile Hydrocarbons. Biochem. Pharmacol 53(110): 1715-1718.
Cruz, J. S., Kushmerick, C., Moreira-Lobo, D. C. and Oliveira, F. A. (2012). Thiamine deficiency in vitro accelerates A-type potassium current inactivation in cerebellar granule neurons. Neuroscience 221: 108-114.
Duke, J. A and Atchley, A. A (1984) proximate analysis. In: Christie, B. R (Ed.), The Handbook of plant science in Agriculture. CRC press, Inc. Boca Raton, Florida.
Duke, J. A. (1985). Proximate analysis. Hand book of medicinal herbs, 7th edition. Livingstone Group, LTD, Edinburgh, 228-229.
Folis, R. H. (1942). “Myocardial necrosis in rats on a potassium low diet prevented by thiamine deficiency”. Bull. John-Hopkins Hospital 71: 235.
Hirunpanich, V., Utaipat, A, molales, N. P. Bunyapraphtsala, N., sato, H., Herunsale, A., et 91 (2006) Hypochoce Sterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L., in hyper cholesterolemic rats. Journal of Ethno- Pharmacology, 103, 252-260.
Janero, D. R. (1990). Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biology Med. 9(6): 515-540.
Jain S. and Hochestein, P. (1980). Free-radical Damage. Arch. Biochem. Biophys. 201:683.
Mohammed, R, Fernandez, J, Pineda, M., & Aguilar, M. (2007). Roselle (Hibiscus sabdariffa) seed oil is a rich source of C-tocopherol. Journal of Food Science, 72 (S): 207-211.
Mohd-Esa, N., Hern, F. S., Ismail, A. And Yee, C. L. (2010). Antioxodant activity in different parts of roselle (Hibiscus sabdariffa L) extracts and potential exploitation of the seeds. Food Chemistry 122: 1055-1060.
Morton, J. F. (1987). Roselle. In fruits of warm climates (pp. 281-286). Miami, USA: Florida Flair Books.
Maduka, H. C. C., Okoye, Z. S. C. and Eje, A. (2003). The influence of Sacoglottis gabonensis stems bark extract and its isolate Bergenin, a Nigerian alcoholic additive, on the metabolic and haematological side effects of 2, 4-dinitrophenylhydrazine-induced tissue damage. Vascular Pharmacology 39: 317-324.
Muhammad, T. B., and Shakib, A. B. (1995). Jus hibiscus: Bukan Sekadarminumanbiasa. Dewan Ekonomi, 12–14.
Ologundudu, A. and Obi, F. O. (2005). Prevention of 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits by orally administered decoction of dried flower of Hibiscus sabdariffa L J. Med. Sci. 5(3): 208-211.
Ologundudu, A., Lawal, A. O., Adesina, O. G. and Obi, F. O. (2006a). Effect of ethanolic extract of Hibiscus sabdariffa L on 2, 4-dinitrophenylhydrazine-induced changes in blood parameters in rabbits. Global J. Pure Appl. Sci. 12(3): 335-338.
Ologundudu, A., Lawal, A. O., Adesina, O. G. and Obi, F. O. (2006b). Effect of ethanolic extract of Hibiscus sabdariffa L on 2, 4-dinitrophenylhydrazine-induced low glucose level and high malondialdehyde levels in rabbit brain and liver. Global J. Pure Appl. Sci. 12(4): 525-529.
Ologundudu, A., Ologundudu, A. O., Oluba, O. M., Omotuyi, I. O. and Obi, F. O. (2010a). Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits. J Toxicol Envir Health Sci 2(1): 1
Ologundudu, A., Ologundudu, A. O., Ololade, I. A. and Obi, F. O. (2009a). Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced hematotoxicity in rabbits. Afr. J. Biochem. Res. 3 (4):140-144.
Ologundudu, A., Ologundudu, A. O., Ololade, I. A. and Obi, F. O. (2009b). The effect of Hibiscus anthocyanins on 2, 4-dinitrophenylhydrazine-induced hepatotoxicity in rabbits. Int. J. Phys. Sci. 4(4): 233-237.
Olusola, A. O. (2014). Effects of Hibiscus sabdariffa calyx anthocyanins and ascorbate on 2, 4-dinitrophenylhydrazine-induced changes in the activities of antioxidant enzymes in rabbits. Research and Reviews Journal of Pharmacology and Toxicological Studies. 2(4):24-30.
Olusola A. O., Olusola, A. O., Bada, S. O. and Obi, F. O. (2012). Comparative study on the effect of Hibiscus sabdariffa calyx anthocyanins and ascorbate on 2, 4 dinitrophenylhydrazine-induced damage in rabbits. American Journal of Biochemistry. 2(2): 1-6.
Olusola A. O., Olusola, A. O., Bada, S. O. and Obi, F. O. (2012). Effects of Hibiscus sabdariffa extracts on 2, 4-dinitrophenylhydrazine-induced cytotoxicity in rabbits. Frontiers in Science. 2(6): 221-225.
Prasongwatana, V., Woottisin, S., Sriboonlue P. and Kukongviriyapan, V. (2008). Uricosuric effect of Roselle (Hibiscus sabdariffa) in normal and renal-stone former subjects. J Ethnopharmacol. 117 (3): 491-495.
Ross I. A. (2003). Hibiscus sabdariffa. In Medicinal Plants of the World, Vol. 1, 2nd Edn. Human Press: New Jersey, pp: 267-275.
SAS Institute Inc. (1999). SAS/STAT User’s Guide. Version 8 for Windows. SAS Institute Inc., SAS Campus Drive, Cary, North Carolina, USA.
Taylor, E. H. (1989). Clinical Chemistry. New York: John Wiley and Sons. Pp. 4, 58-62.
Traynor, J., Mactier, R., Geddes, C. C. and Fox, J. G. (2006). How to measure renal function in clinical practice. BMJ, 333:733.
Walter, F. B. (1998). Medical Physiology: A Cellular And Molecular Approach. Elsevier/Saunder. ISBN 1-4160-2328-3. Page 837.
Vashney, R. and Kale, R. K. (1990). Effect of calmodulin antagonist on radiation induced lipid peroxidation in microsomes. Int. J. Rad. Biol. 58: 733-743.