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International Journal of Clinical Medicine Research

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Snail Extracts Protect Neuroblasts SH-SY5Y Cells from Damage Induced by Hydrogen Peroxide: A Pilot Study

International Journal of Clinical Medicine Research
Vol.5 , No. 3, Publication Date: May 16, 2018, Page: 55-60

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Authors

[1]	Zhao-hui Zhang, Department of Pathology and Pathophysiology, Henan University of Science and Technology, Luoyang, PR China.
[2]	Ping Wang, The Key Laboratory of Pharmacology and Medical Molecular Biology, Henan University of Science and Technology, Luoyang, PR China.
[3]	Qiao-hong Ji, Department of Pathology and Pathophysiology, Henan University of Science and Technology, Luoyang, PR China.
[4]	Ying-li Duan, The Key Laboratory of Pharmacology and Medical Molecular Biology, Henan University of Science and Technology, Luoyang, PR China.
[5]	Shou-min Xi, The Key Laboratory of Pharmacology and Medical Molecular Biology, Henan University of Science and Technology, Luoyang, PR China.

Abstract

The snail serves as a well-documented traditional Chinese medicine for many diseases. However, there has been no report for the potential benefit, especially the neuroprotective effect of the snail polypeptide mixtures (SPM). We made preliminary attempt to explore the protective effect of SPM against cell damage induced by H₂O₂ in neuroblast-drived cell line SH-SY5Y. In the present study, H₂O₂ at the concentration of 1.54 mmol/L significantly induced nuclear condensation, MMP dissipation and decreased expression of PCNA and BDNF. SPM at the concentartion of 39 mg/L and 156 mg/L improved morphological changes of nucleus and MMP dissipation induced by H₂O₂, which was associated with significantly increased expression of PCNA and BDNF. SPM could protect SH-SY5Y cells from apoptosis induced by H₂O₂, which holds promise as a novel drug for the treatment of neurodegenerative diseases.

Keywords

Snail Polypeptide, Hydrogen Peroxide, SH -SY5Y Cell, Apoptosis

Reference

[01]	Gao M, Yang Y, Lv M, Song W, Song Z. Oxidative stress and DNA damage in zebrafish liver due to hydroxyapatite nanoparticles-loaded cadmium. Chemosphere 2018; 202: 498-505.
[02]	Ruan T, Li L, Lyu Y, Luo Q, Wu B. Effect of methionine deficiency on oxidative stress and apoptosis in the small intestine of broilers. Acta Vet Hung 2018; 66: 52-65.
[03]	Ling Y, Gong Q, Xiong X, Sun L, Zhao W, Zhu W, Lu Y. Protective effect of madecassoside on H₂O₂-induced oxidative stress and autophagy activation in human melanocytes. Oncotarget 2017; 8: 51066-51075.
[04]	Yoon JY, Kim DW, Kim EJ, Park BS, Yoon JU, Kim HJ, Park JH. Protective effects of remifentanil against H₂O₂-induced oxidative stress in human osteoblasts. J Dent Anesth Pain Med 2016; 16: 263-271.
[05]	You HJ, Park JH, Pareja-Galeano H, Lucia A, Shin JI. Targeting MicroRNAs Involved in the BDNF Signaling Impairment in Neurodegenerative Diseases. Neuromolecular Med 2016; 18: 540-550.
[06]	Machaalani R, Chen H. Brain derived neurotrophic factor (BDNF), its tyrosine kinase receptor B (TrkB) and nicotine. Neurotoxicology 2018; 65: 186-195.
[07]	Jodeiri FM, Ghaedi K, Megraw TL, Curtiss J, Shirani FM, Vaziri P, Nasr-Esfahani MH. Does PGC1alpha/FNDC5/BDNF Elicit the Beneficial Effects of Exercise on Neurodegenerative Disorders? Neuromolecular Med 2016; 18: 1-15.
[08]	Daigaku Y, Etheridge TJ, Nakazawa Y, Nakayama M, Watson AT, Miyabe I, Ogi T, et al. PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast. PLoS Genet 2017; 13: e1006789.
[09]	Luo Y, Wang Q, Tian P, Jia Y. [Highly expressed CHAF1A and PCNA are positively associated with malignancy of cervical squamous cell carcinoma]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2017; 33: 1696-1701.
[10]	Kashiwaba S, Kanao R, Masuda Y, Kusumoto-Matsuo R, Hanaoka F, Masutani C. USP7 Is a Suppressor of PCNA Ubiquitination and Oxidative-Stress-Induced Mutagenesis in Human Cells. Cell Rep 2015; 13: 2072-2080.
[11]	Kowalska E, Bartnicki F, Fujisawa R, Bonarek P, Hermanowicz P, Tsurimoto T, Muszynska K, et al. Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. Nucleic Acids Res 2018; 46: 25-41.
[12]	Guan SW, Duan LX, Li YY, Wang BX, Zhou QL. A novel polypeptide from Cervus nippon Temminck proliferation of epidermal cells and NIH3T3 cell line. Acta Biochim Pol 2006; 53: 395-397.
[13]	Saez-Freire M, Blanco-Gomez A, Castillo-Lluva S, Gomez-Vecino A, Galvis-Jimenez JM, Martin-Seisdedos C, Isidoro-Garcia M, et al. The biological age linked to oxidative stress modifies breast cancer aggressiveness. Free Radic Biol Med 2018; 120: 133-146.
[14]	Niwa-Kawakita M, Ferhi O, Soilihi H, Le Bras M, Lallemand-Breitenbach V, de The H. PML is a ROS sensor activating p53 upon oxidative stress. J Exp Med 2017; 214: 3197-3206.
[15]	Piri H, Haghdoost-Yazdi H, Fraidouni N, Dargahi T, Yaghoubidoust M, Azadmehr A. The Anti-Parkinsonism Effects of KATP Channel Blockade in the 6-Hydroxydopamine-Induced Animal Model: The Role of Oxidative Stress. Basic Clin Neurosci 2017; 8: 183-192.
[16]	Buzovetsky O, Kwon Y, Pham NT, Kim C, Ira G, Sung P, Xiong Y. Role of the Pif1-PCNA Complex in Pol delta-Dependent Strand Displacement DNA Synthesis and Break-Induced Replication. Cell Rep 2017; 21: 1707-1714.
[17]	Li F, Ball LG, Fan L, Hanna M, Xiao W. Sgs1 helicase is required for efficient PCNA monoubiquitination and translesion DNA synthesis in Saccharomyces cerevisiae. Curr Genet 2018; 64: 459-468.
[18]	Zheng J, Sun J, Lu X, Zhao P, Li K, Li L. BDNF promotes the axonal regrowth after sciatic nerve crush through intrinsic neuronal capability upregulation and distal portion protection. Neurosci Lett 2016; 621: 1-8.