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Vol.4 , No. 1, Publication Date: Jun. 9, 2017, Page: 1-14
 was obtained from 500gms of dry plant material while the phytochemical analysis result revealed the presence of 10 different bioactive constituents viz; alkaloids, flavonoids, saponins, cardiac glycosides, tannins, steroids, terpenes, resins, phenols and volatile oils with the absence of anthraquinones. The crude extract exhibited antibacterial activity against all the test organisms at 20mg/mL with mean inhibition zone (MZI) ranging from 15.00±0.57<sup>a</sup> mm to 20.66±0.33<sup>a</sup> mm, which were effective than the fractions. A total of 8 fractions namely ECO1, ECO2, ECO3, ECO4, ECO5, ECO6, ECO7 and ECO8 were obtained from the crude extract by column chromatography, 5 (ECO2, ECO4, ECO6, ECO7 and ECO8) were active at 40mg/mL against all the test organisms with MZI range of 8.00±0.00mm to 21.66±0.88mm while ECO2 and ECO4 exhibited intermediate activity with MZI range of 15.00±0.00mm 18.66±0.33mm and 9.33±0.33mm to 21.66±0.88mm respectively. The minimum inhibitory concentration (MIC) and corresponding minimum bactericidal concentration (MBC) of the crude extract and fractions were 7.5mg/mL & 120mg/mL and 3.25mg/mL & 120mg/mL respectively. Thirty nine (39) and fifteen (15) compounds were identified in ECO2 and ECO4 respectively by column chromatography with gamma.-Tocopherol (10.25%), Hemimellitene (8.64%), n-Decane (6.82%), 11-Octadecenoic acid, methyl ester (6.56%) & Decane, 2-methyl- (5.13%) and delta. (Sup9)-cis-Oleic acid (21.02%), Stigmasterol, 22,23-dihydro- (14.35%), 6.beta.Bicyclo[4.3.0]nonane, 5.beta.-iodomethyl-1.beta.-isopropenyl-4.alpha.,5.alpha.-dimethyl- (14.13%), n-Hexadecanoic acid (8.62%), Hydrofol Acid 150 (7.64%) and Ricinoleic acid methyl ester (7.61%) as the most abundant respectively. All the identified compounds have been reported to possessing antibacterial activity, therefore, based on the results obtained in this research study and considering the toxicity level of the plant extract with a safe dose (LD50) of 1369mg/kgbw, fractions from <i>Eucalyptus citriodora</i> can be used to develop drug for the treatment of infections caused by the test organisms.&picture=http://www.aascit.org/aascit/decorators/img/journal/906.jpg)
| [1] | Ewansiha Joel Uyi, Department of Microbiology, Federal University of Technology, Yola, Nigeria； Department of Microbiology, Federal University of Technology, Minna, Nigeria. |
| [2] | Garba Samuel Alimi, Department of Microbiology, Federal University of Technology, Minna, Nigeria. |
| [3] | Galadima Musa, Department of Microbiology, Federal University of Technology, Minna, Nigeria. |
| [4] | Daniyan Safiya Yahaya, Department of Microbiology, Federal University of Technology, Minna, Nigeria. |
| [5] | Busari Musa Bola, Centre for Genetic Engineering and Biotechnology (Drug and Vaccine Discovery Unit), Federal University of Technology, Minna, Nigeria. |
| [6] | Doughari James Hamuel, Department of Microbiology, Federal University of Technology, Minna, Nigeria. |
Considering the application of Eucalyptus citriodora in Folklore medicine in the treatment of Typhoid fever and respiratory tract infections, fractions were obtained from n-hexane extract by reflux extraction and chromatography techniques to determine its antibacterial activity and phytochemical properties. A total of 25.85g (5.17%) was obtained from 500gms of dry plant material while the phytochemical analysis result revealed the presence of 10 different bioactive constituents viz; alkaloids, flavonoids, saponins, cardiac glycosides, tannins, steroids, terpenes, resins, phenols and volatile oils with the absence of anthraquinones. The crude extract exhibited antibacterial activity against all the test organisms at 20mg/mL with mean inhibition zone (MZI) ranging from 15.00±0.57a mm to 20.66±0.33a mm, which were effective than the fractions. A total of 8 fractions namely ECO1, ECO2, ECO3, ECO4, ECO5, ECO6, ECO7 and ECO8 were obtained from the crude extract by column chromatography, 5 (ECO2, ECO4, ECO6, ECO7 and ECO8) were active at 40mg/mL against all the test organisms with MZI range of 8.00±0.00mm to 21.66±0.88mm while ECO2 and ECO4 exhibited intermediate activity with MZI range of 15.00±0.00mm 18.66±0.33mm and 9.33±0.33mm to 21.66±0.88mm respectively. The minimum inhibitory concentration (MIC) and corresponding minimum bactericidal concentration (MBC) of the crude extract and fractions were 7.5mg/mL & 120mg/mL and 3.25mg/mL & 120mg/mL respectively. Thirty nine (39) and fifteen (15) compounds were identified in ECO2 and ECO4 respectively by column chromatography with gamma.-Tocopherol (10.25%), Hemimellitene (8.64%), n-Decane (6.82%), 11-Octadecenoic acid, methyl ester (6.56%) & Decane, 2-methyl- (5.13%) and delta. (Sup9)-cis-Oleic acid (21.02%), Stigmasterol, 22,23-dihydro- (14.35%), 6.beta.Bicyclo[4.3.0]nonane, 5.beta.-iodomethyl-1.beta.-isopropenyl-4.alpha.,5.alpha.-dimethyl- (14.13%), n-Hexadecanoic acid (8.62%), Hydrofol Acid 150 (7.64%) and Ricinoleic acid methyl ester (7.61%) as the most abundant respectively. All the identified compounds have been reported to possessing antibacterial activity, therefore, based on the results obtained in this research study and considering the toxicity level of the plant extract with a safe dose (LD50) of 1369mg/kgbw, fractions from Eucalyptus citriodora can be used to develop drug for the treatment of infections caused by the test organisms.
Keywords
Eucalyptus citriodora, Typhoid Fever, Fractions, Chromatography, Bioactivity, N-hexane
Reference
| [01] | Abayomi, T. O., Nneka, N. I., Akinbo, A. A and Joseph, I. O. (2012). Chemical composition and antimicrobial activity of the seed oil of Entandrophragma angolense (Welw). African Journal of Pure and Applied Chemistry; 6(13), pp. 184-187. |
| [02] | Adebanjo, A. O., Aadewumi, C. O & Essien, E.E. (1985) Anti-infective agents in higher plants. 5th International symposium of medicinal plants. University of Ife, Nigeria. 152-158. |
| [03] | Akinyemi, K. O., Oluwa, O. K and Omomigbehin, E. O. (2006). Antimicrobial activity of crude extracts of three medicinal plants used in south-west Nigerian folk medicine on some food borne bacterial pathogens. African Journal of Traditional Complementary and Alternative Medicines; 3 (4): 13-22. |
| [04] | Akpuaka, A., Ekwenchi, M. M., Dashak, D. A and Dildar, A. (2013). Biological Activities of Characterized Isolates of n-Hexane Extract of Azadirachta Indica A.Juss (Neem) Leaves. Nature and Science; 11(5): 1-7. |
| [05] | Aniagu, S.O Nwinyi, F.C & Akumka, D.D (2005). “Toxicity studies in rats fed nature cure bitters,” African Journal of Biotechnology, 4(1) pp. 72–78. |
| [06] | Andrew, P., Desbois & Keelan, C. (2013). Antibacterial Activity of Long-Chain Polyunsaturated Fatty Acids against Propionibacterium acnes and Staphylococcus aureus. Marine drugs, 11: 4544-4557. |
| [07] | Bergsson, G., Arnfinnsson, J., Steingrimsson, O & Thormar, H. (2001). Killing of Gram-positive cocci by fatty acids and monoglycerides. APMIS Journal of pathology, 109:670–678. |
| [08] | Bonjar, G. H. S & Farrokhi, P. R. (2004). Antibacillus activity of some plant used in traditional medicine of Iran. Nigerian Journal on Natural Products and Medicine. (8): 34-39. |
| [09] | Brophy, J. J., Lassak, E. V & Toia, R. F (1985). The steam volatile leaf oil of Eucalyptus pulverulenta. Planta Medica: Journal of Medicinal Plant and natural Product reserch51 (2): 170-171. |
| [10] | Cheesbrough, M. (2002). District Laboratoy Practice in tropical Countries (part 2). University press. Cambridge pp: 180—300. |
| [11] | Clinical and laboratory standard institute-CLSI. (2012). Performance standards for antimicrobial susceptibility testing; seventeenth information supplement (ed.), 27(1); M100-S17. |
| [12] | Dagne, E., Bisrat, D., Alemayehu, M & Worku T. (2000). Essential oils of twelve Eucaluptus species from Ethiopia. Journal of Essential Oil Research; 12: 467-470. |
| [13] | Desbois, A. P & Smith, V. J. (2010). Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85: 1629–1642. |
| [14] | Desbois, A. P. (2012). Potential applications of antimicrobial fatty acids in medicine, agriculture and other industries. Recent Pat. Antiinfective Drug Discovery, 7: 111–122. |
| [15] | Doughari, J. H. (2012). Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents. In: Phytochemicals - A Global Perspective of Their Role in Nutrition and Health. Rao, V (ed), ISBN 978-953-51-0296-0, 548. |
| [16] | Fradin, M. S & Day, J. F. (2002). Comparitive eficiacy of insect repellants against mosquito bites. New England Journal of Medicine. 347(1), 13-18. |
| [17] | Galeotti, F., Barile, E., Curir, P., Dolci, M & Lanzotti, V. (2008). "Flavonoids from carnation (Dianthus caryophyllus) and their antifungal activity". Phytochemistry Letters 1: 44–48. |
| [18] | Galhiane, M. S., Rissato, S. R., Chierice, G. O., Almeida, M. V., & Silva, L.C. (2006). Influence of different extraction methods on the yield and linalool content of the extracts of Eugenia uniflora L. Talanta. 70: 286-292. |
| [19] | Gholamreza, A., Mohamad, J. & Ehsan, S. (2012). Antimicrobial activity and chemical composition of essential oil from the seeds of Artemisia aucheri Boiss. J. Nat Pharm. Prod., 7(1): 11-15. |
| [20] | Gundidza, M., Deans, S. G., Kennedy, A., Mavin, S., Watennam, P. G & Gray A (1993). The essential oil from Hetropyxis natalensis Harv: Its antimicrobial activities and phytoconstituents. Journal of Science and Food Agriculture. 63: 361-364. |
| [21] | Hajir, B. A., Alaa, I. M., Khansa, A. A., Naga, I. A., Wdeea. A & Monier, N. H. (2016). Evolution of Antimicrobial, Antioxidant Potentials and Phytochemical Studies of Three Solvent Extracts of Five Species from Acacia Used in Sudanese Ethnomedicine. Advances in Microbiology, 6: 691-698. |
| [22] | Hatice, Z & Ayse, H. B. (2014). Antibacterial and Antioxidant Activity of Essential Oil Terpenes against Pathogenic and Spoilage-Forming Bacteria and Cell Structure-Activity Relationships Evaluated by SEM Microscopy. Molecules. PP: 1-26. |
| [23] | He, Q., Yao, K., Sun, D & Shi, B. (2007). Biodegradability of tannin-containing waste water from leather industry, Biodegradation, 18: 465-472. |
| [24] | Isaac, C. E. (2005). Human milk inactivate pathogens individually, additively and synergistically. Journal of Nutrition, 135: 1286- 1288. |
| [25] | Johnson, M & Janakiraman, N. (2013). Phytochemical and TLC study on stem and leaves of the orchid Dendrobrum panduratum subsp. Villosum. Indian Journal of Natural Product and Resources, 4(3): 250-254. |
| [26] | Kabir, O. A., Olukayode, O. Chidi. E. O., Christopher, C. I. & Kehinde, A. F. (2005). Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity, BMC Complementary and Alternative Medicine; 5(6): 1-7. |
| [27] | Kawaii, S., Yasuhiko, K T., Eriko, O., Kazunori, Y., Masamichi, K., Meisaku, C & Hiroshi, F. (2000), “Quantitative study of flavonoids in leaves of Citrus plants”, Journal of Agriculture and Food Chemistry; 48: 3865-3871. |
| [28] | Lorke, D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology; 54, 275 – 287. |
| [29] | Luqman, S., Dwivedi, G. R., Darokar, M. P., Kalra, A and Khanuja, S. P. S. (2008). Antimicrobial activity of Eucalyptus citriodora essential oil. International Journal of Essential Oil Therapeutics; 2: 69-75. |
| [30] | Luque de Castro, M. D. & Garcia-Ayuso, L. E. (1998). Soxhlet extraction of solid materials: An outdated technique with a promising innovative future. Analytica Chimica Acta. 369, 1-10. |
| [31] | Mateus, N., Carvalho, E., Luis, C & De Freitas, V. (2004). Influence of the tannin structure on the disruption effect of carbohydrates on protein tannin aggregates, Analytica Chimica Acta 513, 135-140. |
| [32] | Mouton, J. W & Vinks, A. A. (2007). Continuous infusion of beta-lactams. Current Opinion on Critical Care; 13(5): 598-606. |
| [33] | OECD. (2001), Acute Oral Toxicity - Acute toxic class method, Test Guideline No. 423, Organization of Economic Co-Operation and Development (OECD) Guidelines for the Testing of Chemicals, Paris, pp. 1–14. |
| [34] | Oyewole, O. I., Oyedara, O. O., Olabiyi, B. F & Fasanya, T. S. (2013) “Phytochemical, antimicrobial and toxicity studies of Phyllanthus amarus whole plant extract,” International Journal of Bioassays, 2(3): 519–523. |
| [35] | Pavlović, M., Petrović, S., Milenković, M., Couladis, M., Tzakou, O., & Niketić, M. (2011). Chemical composition and antimicrobial activity of Athriscus nemorosa root essential oil. Journal of National Production Communication; 6(2):271-273. |
| [36] | Petshow, B., Batema, R & Ford, L. (1996). Susceptibility of Helicobacter pylori to bactericidal properties of medium chain monoglycerides and free fatty acids. Antinicrobial Agents of Chemotherapy, 40: 302-306. |
| [37] | Rani, P. S. and Agrawal, R. (2006). Volatile compounds of therapeutic importance produced by leuconostoc paramesenteroidses, a native laboratory isolate. Turkish Journal of Biology; 31: 35-40. |
| [38] | Rios, J. L., & Recio, M. C. (2005). Medicinal plants and antimicrobial activity. Journal 0f Ethnopharmacology 100: 80-84. |
| [39] | Robinson, N. (2006). Integrated traditional Chinese medicine. Complement their clinical practice; 12(2): 132-140. |
| [40] | Ruzin, A & Novick, R. (2000). Equivalence of lauric acids and glycerol monolaurate as inhibitors of signal transduction in Staphylococcus aureus. Journal of Bacteriology, 182: 2668-2671. |
| [41] | Sarswati, N. O., Kuldeep, S. N., Lalit, M. T., Puran, S. M., Jai, C. R., Veena, P., Sandeep, S & Amit, P. (2013). Variation in essential oil composition and anti-microbial activity of Indian Oregano (Origanum vulgare L.) population from Indian Himalayan Region (IHR). Journal of Medicinal Plants Research, Vol. 7(46), pp. 3375-3384. |
| [42] | Simon, M. (1993). "The Systematic Distribution of Tannins in the Leaves of Angiosperms: A Tool for Ecological Studies". Journal of Biochemical Systematics and Ecology 21 (8): 833–846. |
| [43] | Sukhdev S. H., Suman, P. S. K., Gennaro, L and Dev, D. R. (2008). Extraction Technologies for Medicinal and Aromatic Plants International. Centre for Science and High Technology, Trieste ed, pp 11. |
| [44] | Trivedi, N. A & Hotchandani, S. C. (2004). A study of the antimicrobial activity of oil of Eucalyptus. Indian Journal of Pharmacy; 36(2): 93-95. |
| [45] | World Health Organization. (2012). Traditional medicine. Fact sheet No. 134. www.who.int/ mediacentre/factsheets/fs134/cn/. |
| [46] | Zarnowski, R., & Suzuki, Y. (2004). Expedient Soxhlet extraction of resorcinolic lipids from wheat grains. Journal of Food Composition and Analysis, 17: 649-664. |
